Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | catalase activity at pH 4.5, in comparison to the other two pH optima at 6.5 and 10.0, has highest sensitivity to azide | Brassica oleracea | |
cyanide | catalase activity at pH 4.5, in comparison to the other two pH optima at 6.5 and 10.0, has lowest sensitivity to cyanide | Brassica oleracea | |
H2O2 | catalase activity at pH 4.5 is inhibited by 5 mM H2O2 and above | Brassica oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.64 | - |
H2O2 | isoenzyme with optimum at pH 6.5 | Brassica oleracea | |
1 | - |
H2O2 | isoenzyme with optimum at pH 4.5 | Brassica oleracea | |
13.4 | - |
H2O2 | isoenzyme with optimum at pH 10.0 | Brassica oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
258000 | - |
isoenzyme with optimum at pH 10.0, SDS-PAGE | Brassica oleracea |
285000 | - |
isoenzyme with optimum at pH 6.5, SDS-PAGE | Brassica oleracea |
308000 | - |
isoenzyme with optimum at pH 4.5, SDS-PAGE | Brassica oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | - |
Brassica oleracea gongylodes, kohlrabi | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bulb | - |
Brassica oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Brassica oleracea | O2 + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | when the extract is incubated at specified temperature (25-70°C) for various length of time (5-30 min) then tested at room temperature a decrease in catalase activity is found at temperature above 50, 60, and 70°C for pHs of 6.5, 10.0, and 4.5, respectively. After heating at 70°C for 30 min, catalase activity loses about 40, 60, and 75% of the original activity at pH 4.5, 10.0, and 6.5, respectively. | Brassica oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
there are three isoenzymes with pH-optima at 4.5, 6.5, and 10.0 | Brassica oleracea |
6.5 | - |
there are three isoenzymes with pH-optima at 4.5, 6.5, and 10.0 | Brassica oleracea |
10 | - |
there are three isoenzymes with pH-optima at 4.5, 6.5, and 10.0 | Brassica oleracea |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
isoenzyme with optimum at pH 4.5 | Brassica oleracea | azide | |
0.075 | - |
isoenzyme with optimum at pH 6.5 | Brassica oleracea | azide | |
0.2 | - |
isoenzyme with optimum at pH 6.5 | Brassica oleracea | cyanide | |
1 | - |
isoenzyme with optimum at pH 10.0 | Brassica oleracea | azide | |
2 | - |
isoenzyme with optimum at pH 10.0 | Brassica oleracea | cyanide | |
12 | - |
isoenzyme with optimum at pH 4.5 | Brassica oleracea | cyanide |