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Literature summary for 1.11.1.6 extracted from

  • Tayefi-Nasrabadi, H.
    Some biochemical properties of catalase from kohlrabi (Brassica oleracea gongylodes) (2008), J. Biol. Sci., 8, 649-653.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
azide catalase activity at pH 4.5, in comparison to the other two pH optima at 6.5 and 10.0, has highest sensitivity to azide Brassica oleracea
cyanide catalase activity at pH 4.5, in comparison to the other two pH optima at 6.5 and 10.0, has lowest sensitivity to cyanide Brassica oleracea
H2O2 catalase activity at pH 4.5 is inhibited by 5 mM H2O2 and above Brassica oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.64
-
H2O2 isoenzyme with optimum at pH 6.5 Brassica oleracea
1
-
H2O2 isoenzyme with optimum at pH 4.5 Brassica oleracea
13.4
-
H2O2 isoenzyme with optimum at pH 10.0 Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
258000
-
isoenzyme with optimum at pH 10.0, SDS-PAGE Brassica oleracea
285000
-
isoenzyme with optimum at pH 6.5, SDS-PAGE Brassica oleracea
308000
-
isoenzyme with optimum at pH 4.5, SDS-PAGE Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
Brassica oleracea gongylodes, kohlrabi
-

Source Tissue

Source Tissue Comment Organism Textmining
bulb
-
Brassica oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
Brassica oleracea O2 + H2O
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 70 when the extract is incubated at specified temperature (25-70°C) for various length of time (5-30 min) then tested at room temperature a decrease in catalase activity is found at temperature above 50, 60, and 70°C for pHs of 6.5, 10.0, and 4.5, respectively. After heating at 70°C for 30 min, catalase activity loses about 40, 60, and 75% of the original activity at pH 4.5, 10.0, and 6.5, respectively. Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
there are three isoenzymes with pH-optima at 4.5, 6.5, and 10.0 Brassica oleracea
6.5
-
there are three isoenzymes with pH-optima at 4.5, 6.5, and 10.0 Brassica oleracea
10
-
there are three isoenzymes with pH-optima at 4.5, 6.5, and 10.0 Brassica oleracea

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
isoenzyme with optimum at pH 4.5 Brassica oleracea azide
0.075
-
isoenzyme with optimum at pH 6.5 Brassica oleracea azide
0.2
-
isoenzyme with optimum at pH 6.5 Brassica oleracea cyanide
1
-
isoenzyme with optimum at pH 10.0 Brassica oleracea azide
2
-
isoenzyme with optimum at pH 10.0 Brassica oleracea cyanide
12
-
isoenzyme with optimum at pH 4.5 Brassica oleracea cyanide