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Literature summary for 1.11.1.6 extracted from

  • Ebara, S.; Shigemori, Y.
    Alkali-tolerant high-activity catalase from a thermophilic bacterium and its overexpression in Escherichia coli (2008), Protein Expr. Purif., 57, 255-260.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, 100-fold increase in active enzyme in presence of 1 mM Mn2+ in the induction medium Metallosphaera hakonensis

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme is not inhibited by presence of 5 mM sodium azide Metallosphaera hakonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, calculated including the putative signal peptide, x * 33000, SDS-PAGE of recombinant inactive form Metallosphaera hakonensis

Organism

Organism UniProt Comment Textmining
Metallosphaera hakonensis A8CFD3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3667
-
25°C, pH 8.0 Metallosphaera hakonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
Metallosphaera hakonensis O2 + H2O
-
?

Subunits

Subunits Comment Organism
? x * 33000, calculated including the putative signal peptide, x * 33000, SDS-PAGE of recombinant inactive form Metallosphaera hakonensis

Synonyms

Synonyms Comment Organism
manganese catalase
-
Metallosphaera hakonensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
50 min, no loss of activity Metallosphaera hakonensis
70
-
50 min, 60% residual activity Metallosphaera hakonensis
80
-
half-life below 10 min Metallosphaera hakonensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Metallosphaera hakonensis

pH Range

pH Minimum pH Maximum Comment Organism
8 10 60 min, 30°C, no loss of activity Metallosphaera hakonensis