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Literature summary for 1.11.1.6 extracted from

  • Lee, D.H.; Oh, D.C.; Oh, Y.S.; Malinverni, J.C.; Kukor, J.J.; Kahng, H.Y.
    Cloning and characterization of monofunctional catalase from photosynthetic bacterium Rhodospirillum rubrum S1 (2007), J. Microbiol. Biotechnol., 17, 1460-1468.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-1,2,4-triazole 10 mM, 26% residual activity Rhodospirillum rubrum
azide
-
Rhodospirillum rubrum
cyanide
-
Rhodospirillum rubrum
hydroxylamine
-
Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
21.8
-
H2O2 pH 7.0, 25°C Rhodospirillum rubrum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron presence of a ferric component Rhodospirillum rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
4 * 61000, SDS-PAGE, 4 * 54000, calculated Rhodospirillum rubrum
61000
-
4 * 61000, SDS-PAGE, 4 * 54000, calculated Rhodospirillum rubrum
189000
-
gel filtration Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
enzyme does not show peroxidase activity
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39960
-
pH 7.0, 25°C Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O
-
Rhodospirillum rubrum O2 + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 61000, SDS-PAGE, 4 * 54000, calculated Rhodospirillum rubrum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Rhodospirillum rubrum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55
-
Rhodospirillum rubrum
60
-
45% of maximum activity Rhodospirillum rubrum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Rhodospirillum rubrum

pH Range

pH Minimum pH Maximum Comment Organism
5 11 more than 50% of maximum activity within Rhodospirillum rubrum

Cofactor

Cofactor Comment Organism Structure
heme
-
Rhodospirillum rubrum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0004
-
pH 7.0, 25°C Rhodospirillum rubrum azide
0.0044
-
pH 7.0, 25°C Rhodospirillum rubrum hydroxylamine
0.0088
-
pH 7.0, 25°C Rhodospirillum rubrum cyanide