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Literature summary for 1.11.1.6 extracted from

  • Mondal, P.; Ray, M.; Kar, M.; Sabat, S.C.
    Molecular identification and properties of a light-insensitive rice catalase-B expressed in E. coli (2008), Biotechnol. Lett., 30, 563-568.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oryza sativa

Protein Variants

Protein Variants Comment Organism
L189W/H225T almost 3fold decrease in Km-value, 2-2.5fold increase in enzyme velocity, loss of photoinhibition Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
H2O2 substrate inhibition, no detectable activity above 60 mM H2O2 Oryza sativa
additional information exposure to white light of nearly 0.800 mE per square meter and second for 120 min at 25°C inactivates the wild-type enzyme activity by about 50% Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
H2O2 mutant L189W/H225T Oryza sativa
11
-
H2O2 wild-type Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000, SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q0D9C4 isoform catalase-B
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
Oryza sativa O2 + 2 H2O
-
r

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Oryza sativa