Literature summary for 1.11.1.6 extracted from
Chandlee, J.M.; Tsaftaris, A.S.; Scandalios, J.G.
Purification and partial characterization of three genetically defined catalases of maize (1983), Plant Sci. Lett., 29, 117-131.
No PubMed abstract available
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
2-mercaptoethanol |
slight inhibition for all three isoforms at 10 mM |
Zea mays |
|
3-amino-1H-1,2,4-triazole |
strong inhibition for isoforms Cat-1, Cat-2, 32% inhibition for isoform Cat-3 at 10 mM |
Zea mays |
|
dithiothreitol |
slight inhibition at 10 mM for all three isoforms |
Zea mays |
|
Endogenous inhibitor |
purified on catalase-Sepharose from bovine, different inhibition sensitivities for three isoforms |
Zea mays |
|
KCN |
strong inhibition for all three isoforms at 1 mM |
Zea mays |
|
NaN3 |
strong inhibition for all three isoforms at 0.001 mM |
Zea mays |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
60000 |
- |
4 * 60000, SDS-PAGE for all three isoforms |
Zea mays |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
H2O2 |
Zea mays |
three isoenzymes, Cat-1, Cat-2 and Cat-3 |
O2 + H2O |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Zea mays |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
to homogeneity, three isoforms |
Zea mays |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
epicotyl |
from seeds |
Zea mays |
- |
kernel |
spadix |
Zea mays |
- |
scutellum |
from seeds |
Zea mays |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
H2O2 |
three isoenzymes, Cat-1, Cat-2 and Cat-3 |
Zea mays |
O2 + H2O |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
tetramer |
4 * 60000, SDS-PAGE for all three isoforms |
Zea mays |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
45 |
- |
Cat-1 half-life: 12.5 min, Cat-3 half-life: 18 min, Cat-2 stable |
Zea mays |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7 |
9 |
three isoforms |
Zea mays |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
4 |
13 |
isoform Cat-2 |
Zea mays |
5 |
12 |
isoforms Cat-1, Cat-3 |
Zea mays |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
6 |
9 |
maximum stability within, isoform Cat-3 |
Zea mays |
7 |
11 |
maximum stability within, isoform Cat-2 |
Zea mays |
7 |
10 |
maximum stability within, isoform Cat-1 |
Zea mays |