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Literature summary for 1.11.1.6 extracted from

  • Chandlee, J.M.; Tsaftaris, A.S.; Scandalios, J.G.
    Purification and partial characterization of three genetically defined catalases of maize (1983), Plant Sci. Lett., 29, 117-131.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol slight inhibition for all three isoforms at 10 mM Zea mays
3-amino-1H-1,2,4-triazole strong inhibition for isoforms Cat-1, Cat-2, 32% inhibition for isoform Cat-3 at 10 mM Zea mays
dithiothreitol slight inhibition at 10 mM for all three isoforms Zea mays
Endogenous inhibitor purified on catalase-Sepharose from bovine, different inhibition sensitivities for three isoforms Zea mays
KCN strong inhibition for all three isoforms at 1 mM Zea mays
NaN3 strong inhibition for all three isoforms at 0.001 mM Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 60000, SDS-PAGE for all three isoforms Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2O2 Zea mays three isoenzymes, Cat-1, Cat-2 and Cat-3 O2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, three isoforms Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
epicotyl from seeds Zea mays
-
kernel spadix Zea mays
-
scutellum from seeds Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2 three isoenzymes, Cat-1, Cat-2 and Cat-3 Zea mays O2 + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, SDS-PAGE for all three isoforms Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
Cat-1 half-life: 12.5 min, Cat-3 half-life: 18 min, Cat-2 stable Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 three isoforms Zea mays

pH Range

pH Minimum pH Maximum Comment Organism
4 13 isoform Cat-2 Zea mays
5 12 isoforms Cat-1, Cat-3 Zea mays

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 maximum stability within, isoform Cat-3 Zea mays
7 11 maximum stability within, isoform Cat-2 Zea mays
7 10 maximum stability within, isoform Cat-1 Zea mays