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Literature summary for 1.11.1.6 extracted from

  • Terzenbach, D.P.; Blaut, M.
    Purification and characterization of a catalase from the nonsulfur phototrophic bacterium Rhodobacter sphaeroides ATH 2.4.1 and its role in the oxidative stress response (1998), Arch. Microbiol., 169, 503-508.
    View publication on PubMed

General Stability

General Stability Organism
resistant up to 7 M urea, 10% SDS Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 36% inhibition at 1 mM Cereibacter sphaeroides
hydroxylamine 85.1% inactivation at 0.005 mM Cereibacter sphaeroides
KCN 72.5% inactivation at 0.005 mM Cereibacter sphaeroides
NaN3 76.4% inactivation at 0.005 mM Cereibacter sphaeroides
Sodium dithionite inhibitory Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
40
-
H2O2
-
Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
1 * 75000 + 1 * 68000, SDS-PAGE Cereibacter sphaeroides
75000
-
1 * 75000 + 1 * 68000, SDS-PAGE Cereibacter sphaeroides
150000
-
native PAGE Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2O2 Cereibacter sphaeroides no peroxidase-activity O2 + H2O
-
?
H2O2 Cereibacter sphaeroides monofunctional catalase O2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Cereibacter sphaeroides

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible by H2O2, heat-shock, ethanol and stationary-phase conditions Cereibacter sphaeroides
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
55930
-
-
Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
4°C, 90% of the activity lost in 14 days Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2 no peroxidase-activity Cereibacter sphaeroides O2 + H2O
-
?
H2O2 monofunctional catalase Cereibacter sphaeroides O2 + H2O
-
?

Subunits

Subunits Comment Organism
dimer 1 * 75000 + 1 * 68000, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
54
-
-
Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
50% loss of activity in 30 min Cereibacter sphaeroides
80
-
complete loss of activity Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 10
-
Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
heme protoheme IX Cereibacter sphaeroides