General Stability | Organism |
---|---|
resistant up to 7 M urea, 10% SDS | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 36% inhibition at 1 mM | Cereibacter sphaeroides | |
hydroxylamine | 85.1% inactivation at 0.005 mM | Cereibacter sphaeroides | |
KCN | 72.5% inactivation at 0.005 mM | Cereibacter sphaeroides | |
NaN3 | 76.4% inactivation at 0.005 mM | Cereibacter sphaeroides | |
Sodium dithionite | inhibitory | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
H2O2 | - |
Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
1 * 75000 + 1 * 68000, SDS-PAGE | Cereibacter sphaeroides |
75000 | - |
1 * 75000 + 1 * 68000, SDS-PAGE | Cereibacter sphaeroides |
150000 | - |
native PAGE | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Cereibacter sphaeroides | no peroxidase-activity | O2 + H2O | - |
? | |
H2O2 | Cereibacter sphaeroides | monofunctional catalase | O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Cereibacter sphaeroides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | inducible by H2O2, heat-shock, ethanol and stationary-phase conditions | Cereibacter sphaeroides | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
55930 | - |
- |
Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
4°C, 90% of the activity lost in 14 days | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | no peroxidase-activity | Cereibacter sphaeroides | O2 + H2O | - |
? | |
H2O2 | monofunctional catalase | Cereibacter sphaeroides | O2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 75000 + 1 * 68000, SDS-PAGE | Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | - |
- |
Cereibacter sphaeroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
50% loss of activity in 30 min | Cereibacter sphaeroides |
80 | - |
complete loss of activity | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | protoheme IX | Cereibacter sphaeroides |