Application | Comment | Organism |
---|---|---|
drug development | LDH is a potential drug target and candidate antigen for immunodiagnosis (LDH can be recognized in serum from swine or patient infected with Taenia asiatica) and vaccine for taeniasis and viscero-cysticercosis caused by Taenia asiatica | Taenia asiatica |
Cloned (Comment) | Organism |
---|---|
into plasmid pET-30a and expressed with a His tag in Escherichia coli BL21/DE3 | Taenia asiatica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
NAD+ | - |
Taenia asiatica | |
0.32 | - |
NADH | - |
Taenia asiatica | |
1.88 | - |
pyruvate | - |
Taenia asiatica | |
10.26 | - |
Lactate | - |
Taenia asiatica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | embryonic membrane of oncosphere | Taenia asiatica | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35200 | - |
sequence analysis | Taenia asiatica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taenia asiatica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by His bind purification | Taenia asiatica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | embryonic membrane of oncosphere | Taenia asiatica | - |
additional information | surface of the tegument in adults. Is absent from internal tissues | Taenia asiatica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
456 | - |
purified protein with pyruvate as substrate | Taenia asiatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Taenia asiatica | lactate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lactate dehydrogenase | - |
Taenia asiatica |
LDH | - |
Taenia asiatica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
highest relative activity for both the reduction and oxidation reactions | Taenia asiatica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 65 | optimal temperatures for the reduction and oxidation reactions are 37-65°C and 40-50°C, respectively | Taenia asiatica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | optimal pH range for pyruvate reduction and lactate oxidation is 6.5-7.0 and 9.0-10.0, respectively | Taenia asiatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Taenia asiatica | |
NADH | - |
Taenia asiatica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Taenia asiatica | theoretical isoelectric point | - |
8.03 |