Application | Comment | Organism |
---|---|---|
industry | cofactor recycling catalytic system reveals both 100% selectivity and aldehyde conversion within 3 h by usage of ADH, which provides an inexpensive overall process | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cinnamaldehyde | substrate inhibition mediated by cinnamaldehyde concentrations far from the Km value of 0.46 mM, which does not allow for excessively increasing the starting cinnamaldehyde concentration, since the process yield may be greatly affected | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
NADH | - |
Saccharomyces cerevisiae | |
0.46 | - |
cinnamaldehyde | - |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-propanol + NADP+ | 2-propanol can be also used in the cofactor recycling catalytic system as co-solvent like ethanol, but at the same time cinnamaldehyde conversion is lower (85%) and cinnamyl alcohol production is accordingly lower | Saccharomyces cerevisiae | ? + NADPH + H+ | - |
? | |
cinnamaldehyde + NADH + H+ | cofactor recycling catalytic system in which ADH reduces cinnamaldehyde to cinnamyl alcohol (oxidizing NADH to NAD+). THe NAD+ produced is then recycled by ADH at expense of ethanol, which acts as co-solvent for both substrate and product, and is present in large excess to force the whole process toward cinnamaldehyde reduction. Also, elimination of the obtained dehydrogenation product acetaldehyde drives cinnamaldehyde reduction toward completion. 1-2 mM is the ideal starting cinnamaldehyde concentration | Saccharomyces cerevisiae | cinnamyl alcohol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Saccharomyces cerevisiae |
alcohol dehydrogenase | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | up to 30°C, retains all initial catalytic activity, only 18% activity is lost at 40°C, at 60°C 100% loss of activity | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
optimum for cinnamaldehyde reduction, whereas oxidation of ethanol and 2-propanol is best accomplished at pH 9, pH 7 is a satisfactory compromise | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
no significant activity loss up to 24 h | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Saccharomyces cerevisiae |