Information on EC 1.1.1.195 - cinnamyl-alcohol dehydrogenase

Word Map on EC 1.1.1.195
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.195
-
RECOMMENDED NAME
GeneOntology No.
cinnamyl-alcohol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
capsiconiate biosynthesis
-
-
Metabolic pathways
-
-
phenylpropanoid biosynthesis
-
-
Phenylpropanoid biosynthesis
-
-
phenylpropanoid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
cinnamyl-alcohol:NADP+ oxidoreductase
Acts on coniferyl alcohol, sinapyl alcohol, 4-coumaryl alcohol and cinnamyl alcohol (cf. EC 1.1.1.194 coniferyl-alcohol dehydrogenase).
CAS REGISTRY NUMBER
COMMENTARY hide
55467-36-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Abies nobilis
-
-
-
Manually annotated by BRENDA team
A3 and A6
-
-
Manually annotated by BRENDA team
M20 and M22
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Corylus sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Eucalyptus sp.
-
-
-
Manually annotated by BRENDA team
gene Fxcad1; cv. Chandler, octaploid cultivar, gene Fxcad1 and Fxcad2
SwissProt
Manually annotated by BRENDA team
var. Mandarin
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene Gold-hull-and-internode2, i.e. gene GH2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Japanese black pine
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Pteris wimsetti
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Sphagnum sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain PCC 6803
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
neoformed calli induced by Agrobacterium rhizogenes on Phaseolus mungo hypocotyl
-
-
Manually annotated by BRENDA team
cv. Canary Bird
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dimethylcinnamyl alcohol + NADP+
2,4-dimethylcinnamaldehyde + NADPH
show the reaction diagram
-
-
-
-
2-propanol + NADP+
? + NADPH + H+
show the reaction diagram
-
2-propanol can be also used in the cofactor recycling catalytic system as co-solvent like ethanol, but at the same time cinnamaldehyde conversion is lower (85%) and cinnamyl alcohol production is accordingly lower
-
-
-
3,4-dimethoxycinnamyl alcohol + NADP+
3,4-dimethoxycinnamaldehyde + NADPH
show the reaction diagram
3,4-methylenedioxycinnamyl alcohol + NADP+
3,4-methylenedioxycinnamaldehyde + NADPH
show the reaction diagram
-
-
-
-
3-methoxybenzylalcohol + NADP+
3-methoxybenzaldehyde + NADPH
show the reaction diagram
-
-
-
-
3-phenyl-1-propanol + NAD+
3-phenylpropanal + NADH + H+
show the reaction diagram
4-bromocinnamyl alcohol + NADP+
4-bromocinnamaldehyde + NADPH
show the reaction diagram
-
-
-
-
4-chlorocinnamyl alcohol + NADP+
4-chlorocinnamaldehyde + NADPH
show the reaction diagram
-
-
-
-
4-coumaraldehyde + NADPH + H+
4-coumaryl alcohol + NADP+
show the reaction diagram
-
-
-
?
4-coumarylaldehyde + NADPH + H+
4-coumaryl alcohol + NADP+
show the reaction diagram
4-methoxybenzylalcohol + NADP+
4-methoxybenzaldehyde + NADPH
show the reaction diagram
-
-
-
-
4-methoxycinnamyl alcohol + NADP+
4-methoxycinnamaldehyde + NADPH
show the reaction diagram
-
-
-
-
4-methylcinnamyl alcohol + NADP+
4-methylcinnamaldehyde + NADPH
show the reaction diagram
-
-
-
-
5-hydroxyconiferyl aldehyde + NADPH
5-hydroxyconiferyl alcohol + NADP+
show the reaction diagram
-
-
-
r
5-hydroxyconiferyl aldehyde + NADPH + H+
5-hydroxyconiferyl alcohol + NADP+
show the reaction diagram
-
-
-
r
5-hydroxyconiferylaldehyde + NADPH + H+
5-hydroxyconiferyl alcohol + NADP+
show the reaction diagram
-
lowest catalytic efficiency
-
-
?
allyl alcohol + NAD+
prop-2-enal + NADH + H+
show the reaction diagram
benzaldehyde + NADP+
benzoic acid + NADPH
show the reaction diagram
-
the enzyme also shows dismutase activity
-
-
?
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
-
-
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
CAD4 displays slight activity for benzoyl alcohol
-
-
?
benzyl alcohol + NADP+
benzylaldehyde + NADPH + H+
show the reaction diagram
slight activity
-
-
r
benzylalcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
-
-
-
-
butanol + NADP+
butyraldehyde + NADPH
show the reaction diagram
-
-
-
-
r
caffeoyl aldehyde + NADPH + H+
caffeoyl alcohol + NADP+
show the reaction diagram
-
-
-
r
caffeyl aldehyde + NADPH
caffeyl alcohol + NADP+
show the reaction diagram
-
-
-
r
caffeylaldehyde + NADPH + H+
caffeyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
show the reaction diagram
cinnamaldehyde + NADPH
cinnamyl alcohol + NADP+
show the reaction diagram
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH + H+
show the reaction diagram
-
-
-
-
r
cinnamyl alcohol + NADP+
cinnamaldehyde + NADPH
show the reaction diagram
cinnamyl alcohol + NADP+
cinnamaldehyde + NADPH + H+
show the reaction diagram
cinnamyl alcohol + NADP+
cinnamyl aldehyde + NADPH
show the reaction diagram
cinnamyl alcohol + NADP+
cinnamyl aldehyde + NADPH + H+
show the reaction diagram
-
-
-
?
cinnamyl aldehyde + NADPH
cinnamyl alcohol + NADP+
show the reaction diagram
-
-
-
r
coniferaldehyde + NADPH + H+
coniferyl alcohol + NADP+
show the reaction diagram
coniferol + NADP+
coniferyl aldehyde + NADPH
show the reaction diagram
-
-
-
?
coniferyl alcohol + NADP+
coniferyl aldehyde + NADPH + H+
show the reaction diagram
coniferyl alcohol and NADP+
coniferyl aldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
?
coniferyl aldehyde + NADPH
coniferol + NADP+
show the reaction diagram
coniferyl aldehyde + NADPH
coniferyl alcohol + NADP+
show the reaction diagram
coniferyl aldehyde + NADPH + H+
coniferyl alcohol + NADP+
show the reaction diagram
coumaryl alcohol + NADP+
coumaraldehyde + NADPH + H+
show the reaction diagram
-
-
-
?
coumaryl alcohol + NADP+
coumaryl aldehyde + NADPH + H+
show the reaction diagram
coumaryl aldehyde + NADPH + H+
coumaryl alcohol + NADP+
show the reaction diagram
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
r
p-coumaryl alcohol + NADP+
p-coumaraldehyde + NADPH
show the reaction diagram
p-coumaryl aldehyde + NADPH + H+
p-coumaryl alcohol + NADP+
show the reaction diagram
-
-
-
r
propanol + NADP+
propionaldehyde + NADPH
show the reaction diagram
-
-
-
-
r
sinapaldehyde + NADPH + H+
sinapyl alcohol + NADP+
show the reaction diagram
sinapyl alcohol + NADP+
sinapaldehyde + NADPH
show the reaction diagram
sinapyl alcohol + NADP+
sinapyl aldehyde + NADPH + H+
show the reaction diagram
sinapyl aldehyde + NADPH + H+
sinapyl alcohol + NADP+
show the reaction diagram
sinapylaldehyde + NADPH + H+
sinapyl alcohol + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
show the reaction diagram
-
-
-
-
r
cinnamaldehyde + NADPH
cinnamyl alcohol + NADP+
show the reaction diagram
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH + H+
show the reaction diagram
-
-
-
-
r
cinnamyl alcohol + NADP+
cinnamyl aldehyde + NADPH
show the reaction diagram
cinnamyl aldehyde + NADPH
cinnamyl alcohol + NADP+
show the reaction diagram
O49482, P48523
-
-
-
r
coniferaldehyde + NADPH + H+
coniferyl alcohol + NADP+
show the reaction diagram
-
isoform CAD3 has a higher binding preference with coniferaldehyde over sinapaldehyde, followed by isoforms CAD4, CAD2, and CAD1, respectively
-
-
?
coniferol + NADP+
coniferyl aldehyde + NADPH
show the reaction diagram
Q02971
-
-
-
?
coniferyl alcohol + NADP+
coniferyl aldehyde + NADPH + H+
show the reaction diagram
coniferyl aldehyde + NADPH
coniferyl alcohol + NADP+
show the reaction diagram
sinapaldehyde + NADPH + H+
sinapyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
sinapyl alcohol + NADP+
sinapyl aldehyde + NADPH + H+
show the reaction diagram
Q02971
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
NADPH is about 4000fold less effective than NADH
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
activates the enzyme activity in vivo by 45% after a 40 days treatment of roots with over 0.05 mM Cu2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine 2',5'-diphosphate
-
-
adenosine 5'-monophosphate
-
IC50: 1.58 mM with NADP+ and 0.74 mM with NAD+
benzyl alcohol
-
competitive mechanism, substrate inhibition
cinnamaldehyde
-
substrate inhibition mediated by cinnamaldehyde concentrations far from the Km value of 0.46 mM, which does not allow for excessively increasing the starting cinnamaldehyde concentration, since the process yield may be greatly affected
cinnamyl alcohol
-
competitive mechanism, substrate inhibition
coniferin
-
with coniferyl alcohol as substrates, noncompetitive inhibition
coniferyl alcohol
-
competitive mechanism, substrate inhibition
Fe3+
-
1 mM, 74% residual activity
flavones
Eucalyptus sp.
-
IC50: 0.07-0.055 mM
iodoacetic acid
-
1 mM, 56% residual activity
N-bromosuccimide
-
1 mM, 56% residual activity
Ni
-
CAD activity is not affected by concentrations up to 0.12 mM, phenolic metabolism is not substantially affected by Ni excess
RNAi
-
RNAi-mediated transient gene silencing in the epidermis leads to a higher penetration efficiency of Blumeria graminis f. sp. tritici (64%) than in the controls. Gene silencing also compromises penetration resistance to varying degrees with different genes against an inappropriate pathogen, Blumeria graminis f. sp. hordei. Co-silencing of CAD and other genes involved in monolignol biosynthesis leads to greater penetration of Blumeria graminis f. sp. tritici or Blumeria graminis f. sp. hordei than when the genes are silenced separately. Gene silencing hamperes host autofluorescence response at fungal contact sites
-
Zn2+
-
1 mM, 80% residual activity
[[(2-hydroxyphenyl) amino]sulphinyl] acetic acid, 1.1 dimethyl ester
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CO2
-
high CO2 stimulates progressively the enzyme, as well as of other enzymes involved in metabolism of phenolic compounds, overview
jasmonate
accumulation of CAD mRNA in response to jasmonate application and mechanical wounding
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036
3,4-dimethoxy-cinnamyl alcohol
-
-
0.0069 - 0.043
3,4-dimethoxycinnamaldehyde
0.022
3,4-dimethoxycinnamyl alcohol
-
-
0.106
3-Methoxybenzaldehyde
-
-
0.96
3-Phenyl-1-propanol
-
pH 8.0, 30°C
0.013 - 0.117
4-coumaraldehyde
0.0382 - 0.1122
4-coumarylaldehyde
-
0.13
4-methoxybenzaldehyde
-
-
0.034
4-methoxycinnamyl alcohol
-
-
0.0244
5-hydroxyconiferyl aldehyde
pH not specified in the publication, temperature not specified in the publication
0.09318 - 0.152
5-hydroxyconiferylaldehyde
-
0.04
acetaldehyde
-
pH 7.5, 37°C, recombinant enzyme
0.5
allyl alcohol
-
pH 8.0, 30°C
0.03 - 31
benzaldehyde
0.41 - 2.6
benzyl alcohol
9
butanol
-
pH 7.5, 37°C, recombinant enzyme
0.0891
caffeoyl aldehyde
pH not specified in the publication, temperature not specified in the publication
0.06592 - 0.09463
caffeylaldehyde
-
0.0025 - 0.92
cinnamaldehyde
0.0122 - 0.27
cinnamyl alcohol
0.005
cinnamyl aldehyde
-
pH 7.5, 37°C, recombinant enzyme
0.00077 - 0.1136
coniferaldehyde
0.00098 - 0.677
coniferyl alcohol
0.0018 - 0.053
coniferyl aldehyde
0.0042
coumaryl alcohol
pH not specified in the publication, temperature not specified in the publication
46
ethanol
-
pH 7.5, 37°C, recombinant enzyme
0.052 - 1.23
NAD+
0.016 - 0.071
NADH
0.0038 - 0.06
NADP+
0.0046 - 0.15
NADPH
0.0012 - 0.03
p-coumaraldehyde
0.034 - 0.283
p-coumaryl alcohol
0.0272
p-coumaryl aldehyde
pH not specified in the publication, temperature not specified in the publication
13
Propanol
-
pH 7.5, 37°C, recombinant enzyme
0.0043 - 0.2082
sinapaldehyde
0.0156 - 0.56
sinapyl alcohol
0.005 - 0.0406
sinapyl aldehyde
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.2
3-Phenyl-1-propanol
Streptomyces sp.
-
pH 8.0, 30°C
0.026 - 74.2
4-coumaraldehyde
0.273 - 0.367
4-coumarylaldehyde
-
0.005 - 0.007
5-hydroxyconiferylaldehyde
-
16.7
acetaldehyde
Helicobacter pylori
-
pH 7.5, 37°C, recombinant enzyme
32.9
allyl alcohol
Streptomyces sp.
-
pH 8.0, 30°C
2.3 - 2.5
benzaldehyde
8.4
benzyl alcohol
Helicobacter pylori
-
pH 7.5, 37°C, recombinant enzyme
5.7
butanol
Helicobacter pylori
-
pH 7.5, 37°C, recombinant enzyme
0.062 - 0.072
caffeylaldehyde
-
14.4 - 107.9
cinnamaldehyde
13.3 - 60.78
cinnamyl alcohol
7.7
cinnamyl aldehyde
Helicobacter pylori
-
pH 7.5, 37°C, recombinant enzyme
0.072 - 12
coniferaldehyde
0.43 - 13.2
coniferyl alcohol
0.17 - 138
coniferyl aldehyde
24.7
coumaryl alcohol
Panicum virgatum
E2DIF4, E2DIF5
pH not specified in the publication, temperature not specified in the publication
7.1
ethanol
Helicobacter pylori
-
pH 7.5, 37°C, recombinant enzyme
0.0242 - 33
NAD+
43
NADH
Geobacillus stearothermophilus
-
pH 7.5, 50°C
0.0555 - 15.5
NADP+
0.49 - 25.2
NADPH
12.8
Propanol
Helicobacter pylori
-
pH 7.5, 37°C, recombinant enzyme
0.005 - 8.3
sinapaldehyde
46.4
sinapyl alcohol
Panicum virgatum
E2DIF4, E2DIF5
pH not specified in the publication, temperature not specified in the publication
3.4 - 196
sinapyl aldehyde
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.6
3-Phenyl-1-propanol
Streptomyces sp.
-
pH 8.0, 30°C
5108
0.23 - 2061
4-coumaraldehyde
6121
3.273 - 7.136
4-coumarylaldehyde
213965
0.169
5-hydroxyconiferyl aldehyde
Triticum aestivum
D7PGW0
pH not specified in the publication, temperature not specified in the publication
14005
0.0448 - 0.054
5-hydroxyconiferylaldehyde
213967
66.5
allyl alcohol
Streptomyces sp.
-
pH 8.0, 30°C
3612
0.082
caffeoyl aldehyde
Triticum aestivum
D7PGW0
pH not specified in the publication, temperature not specified in the publication
5329
0.766 - 0.935
caffeylaldehyde
213966
15.6 - 1835
cinnamaldehyde
1071
106 - 391
cinnamyl alcohol
936
0.993 - 210
coniferaldehyde
3680
13400
coniferyl alcohol
Panicum virgatum
E2DIF4, E2DIF5
pH not specified in the publication, temperature not specified in the publication
1202
0.896 - 35800
coniferyl aldehyde
1677
5880
coumaryl alcohol
Panicum virgatum
E2DIF4, E2DIF5
pH not specified in the publication, temperature not specified in the publication
12482
73 - 387
NAD+
7
2687
NADH
Geobacillus stearothermophilus
-
pH 7.5, 50°C
8
0.25
p-coumaryl aldehyde
Triticum aestivum
D7PGW0
pH not specified in the publication, temperature not specified in the publication
84014
0.063 - 170
sinapaldehyde
5214
2980
sinapyl alcohol
Panicum virgatum
E2DIF4, E2DIF5
pH not specified in the publication, temperature not specified in the publication
3670
0.286 - 20500
sinapyl aldehyde
5313
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011 - 0.45
adenosine 2',5'-diphosphate
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.58
adenosine 5'-monophosphate
Eucalyptus gunnii
-
IC50: 1.58 mM with NADP+ and 0.74 mM with NAD+
0.07 - 0.055
flavones
Eucalyptus sp.
-
IC50: 0.07-0.055 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00007
-
crude extract from young etiolated shoots
0.00125
-
induced cell culture
0.0014
-
substrate coniferyl aldehyde, recombinant enzyme encoded by GH2
0.0034
-
crude extract from bean pods
0.0036
-
substrate sinapyl aldehyde, recombinant enzyme encoded by GH2
0.006
-
elicitor-treated wheat leaves
0.18
-
crude extract from differentiating xylem
0.33
-
recombinant enzyme, with NAD+ as cofactor
0.39
-
S212D mutant, with NAD+ as cofactor
1.67
-
S212D mutant, with NADP+ as cofactor
3.86
-
-
4.7
-
recombinant enzyme, with NADP+ as cofactor
56.4
-
-
64.56
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
isoform CAD2
6.25
assay at, forward reaction; assay at, forward reaction
9
-
coniferyl alcohol oxidation dependent on ionic strength
10.5
reverse reaction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 8.5
-
pH 4.5: about 55% of activity maximum, pH 8.5: about 30% of activity maximum
6 - 8
-
coniferyl aldehyde reduction, pH 6.0: about 70% of activity maximum, pH 8.0: about 30% of activity maximum
7.2 - 9.7
-
coniferyl alcohol oxidation, pH 7.2: about 40% of activity maximum, pH 9.7: about 35% of activity maximum
8
more than 70% of the maximum activity
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
about 85% loss of initial activity
60
complete loss of activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
calculated
5.8
calculated
6
-
isoform CAD3, calculated from amino acid sequence
6.2
-
isoform CAD2, calculated from amino acid sequence
6.3
-
calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
shell zone, immunogold-electron microscopy of shoot apex
Manually annotated by BRENDA team
-
CAD is accumulated in the epidermis, CAD gene expression after pathogen attack is predominantly in the epidermis
Manually annotated by BRENDA team
expression in all fruit ripening stages
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
expression of isoforms Cad-2, Cad-3
Manually annotated by BRENDA team
-
all CAD genes expressed, but at different levels
Manually annotated by BRENDA team
-
immunogold-electron microscopy of shoot apex
Manually annotated by BRENDA team
-
developing, immunogold-electron microscopy of shoot apex
Manually annotated by BRENDA team
-
immunogold-electron microscopy of mature stems
Manually annotated by BRENDA team
-
developing
Manually annotated by BRENDA team
-
immunogold-electron microscopy of shoot apex
Manually annotated by BRENDA team
-
differentiating element
Manually annotated by BRENDA team
-
predominant expression of isoforms Cad-1, Cad-9
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
14 of the 15 CAD genes are distributed on duplicated regions
-
Manually annotated by BRENDA team