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Information on EC 4.4.1.5 - lactoylglutathione lyase and Organism(s) Sus scrofa

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EC Tree
IUBMB Comments
Also acts on 3-phosphoglycerol-glutathione.
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This record set is specific for:
Sus scrofa
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Word Map
The taxonomic range for the selected organisms is: Sus scrofa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
glyoxalase i, glyoxalase 1, glo-1, glo-i, glo i, glyoxalase-i, glyoxalase-1, gly-i, lactoylglutathione lyase, glyoxylase i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aldoketomutase
-
-
-
-
GLXI
-
-
-
-
glyoxalase I
-
-
-
-
glyoxylase I
-
-
-
-
ketone-aldehyde mutase
-
-
-
-
lyase, lactoylglutathione
-
-
-
-
methylglyoxalase
-
-
-
-
methylglyoxylase
-
-
-
-
S-D-lactoylglutathione methylglyoxal lyase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-S-lactoylglutathione = glutathione + 2-oxopropanal
show the reaction diagram
mechanism
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
(R)-S-lactoylglutathione methylglyoxal-lyase (isomerizing; glutathione-forming)
Also acts on 3-phosphoglycerol-glutathione.
CAS REGISTRY NUMBER
COMMENTARY hide
9033-12-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glutathione + methylglyoxal
S-lactoylglutathione
show the reaction diagram
glutathione + phenylglyoxal
S-mandeloylglutathione
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
activity with glutathione analogs
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activates apoenzyme
Mg2+
-
activates apoenzyme
Mn2+
-
activates apoenzyme
Zn2+
-
metalloenzyme with one Zn2+ per subunit
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
reactivation by Mg2+, Mn2+ or Ca2+
GSH
-
non-linear inhibition
additional information
-
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
methylglyoxal
-
-
0.1
Phenylglyoxal
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
983
methylglyoxal
-
-
617
Phenylglyoxal
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
159
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
I3LDM7_PIG
184
0
20731
TrEMBL
other Location (Reliability: 2)
A0A8D0I2F2_PIG
183
0
20849
TrEMBL
other Location (Reliability: 2)
A0A8D0PDF1_PIG
184
0
20731
TrEMBL
other Location (Reliability: 2)
A0A8D0LDV4_PIG
181
0
20348
TrEMBL
other Location (Reliability: 3)
A0A4X1SRH7_PIG
253
0
28752
TrEMBL
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
-
2 * 24000, SDS-PAGE
48000
-
gel filtration
52000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 24000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
vander Jagt, D.L.
The glyoxalase system
Coenzymes and cofactors, Glutathione, Chem. Biochem. Med. Aspects Pt. A (Dolphin D, Poulson R, Avromonic O, eds. ) John Wiley & Sons, New York
3
597-641
1989
Saccharomyces cerevisiae, Oryctolagus cuniculus, Escherichia coli, Ovis aries, Homo sapiens, Mus musculus, Rattus norvegicus, Rhodospirillum rubrum, Sus scrofa
-
Manually annotated by BRENDA team
Marmstal, E.; Aronsson, A.C.; Mannervik, B.
Comparison of glyoxalase I purified from yeast (Saccharomyces cerevisiae) with the enzyme from mammalian sources
Biochem. J.
183
23-30
1979
Saccharomyces cerevisiae, Homo sapiens, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Aronsson, A.C.; Mannervik, B.
Characterization of glyoxalase I purified from pig erythrocytes by affinity chromatography
Biochem. J.
165
503-509
1977
Sus scrofa
Manually annotated by BRENDA team
Mannervik, B.; Lindstrm, L.; Bartfai, T.
Partial purification and characterization of glyoxalase I from porcine erythrocytes
Eur. J. Biochem.
29
276-281
1972
Sus scrofa
Manually annotated by BRENDA team
Ekwall, K.; Mannervik, B.
The stereochemical configuration of the lactoyl group of S-lactoylglutathionine formed by the action of glyoxalase I from porcine erythrocytes and yeast
Biochim. Biophys. Acta
297
297-299
1973
Saccharomyces cerevisiae, Sus scrofa
Manually annotated by BRENDA team
Shih, M.J.; Edinger, J.W.; Creighton, D.J.
Diffusion-dependent kinetic properties of glyoxalase I and estimates of the steady-state concentrations of glyoxalase-pathway intermediates in glycolyzing erythrocytes
Eur. J. Biochem.
244
852-857
1997
Anser sp., Bos taurus, Saccharomyces cerevisiae, Canis lupus familiaris, Cavia porcellus, Gallus gallus, Oryctolagus cuniculus, Ovis aries, Macaca mulatta, Meleagris gallopavo, Mus musculus, Sus scrofa
Manually annotated by BRENDA team