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Information on EC 3.6.4.13 - RNA helicase and Organism(s) Mus musculus
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3.6.4.13 RNA helicaseIUBMB Comments
RNA helicases utilize the energy from ATP hydrolysis to unwind RNA. Some of them unwind RNA with a 3' to 5' polarity , other show 5' to 3' polarity . Some helicases unwind DNA as well as RNA [7,8]. May be identical with EC 3.6.4.12 (DNA helicase).
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Word Map
- 3.6.4.13
-
single-stranded
- strand
- viruses
- duplex
- fork
-
retinoic
- nucleic
- chromatin
-
acid-inducible
- mda5
- dsrnas
- ssdna
-
rna-binding
-
stall
-
werner
-
nonstructural
-
bloom
- polyproteins
-
dna-dependent
- primase
-
exonuclease
- replisome
-
spliceosome
-
hexameric
-
minichromosome
-
differentiation-associated
- dengue
-
g-quadruplexes
- eif4g
-
single-molecule
-
unwound
-
replicase
-
restart
-
ifn-stimulated
-
nonsense-mediated
- rpa
- rad51
-
holliday
-
polyi:c
- flavivirus
- tfiih
- exoribonuclease
-
ifn-beta
-
d-loops
- pigmentosum
-
cap-dependent
- xeroderma
-
positive-stranded
- pre-rrnas
-
overhang
- pharmacology
- medicine
- drug development
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
helicase, rig-i, rna helicase, eif4a, ddx3x, dead-box rna helicase, ns3 helicase, dead-box helicase, ddx21, rna helicase a, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
DEAD box RNA helicase
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (RNA helix unwinding)
RNA helicases utilize the energy from ATP hydrolysis to unwind RNA. Some of them unwind RNA with a 3' to 5' polarity [3], other show 5' to 3' polarity [8]. Some helicases unwind DNA as well as RNA [7,8]. May be identical with EC 3.6.4.12 (DNA helicase).
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
helicase DDX6 colocalizes with TRIM32 in neural stem cells and neurons and increases the activity of Let-7a. The activation of Let-7a depends on the enzyme's helicase activity
-
-
?
ATP + H2O
ADP + phosphate
the ability of RNA helicases to modulate the structure and thus availability of critical RNA molecules for processing leading to protein expression is the likely mechanism by which RNA helicases contribute to differentiation
-
-
?
ATP + H2O
ADP + phosphate
the ability of RNA helicases to modulate the structure and thus availability of critical RNA molecules for processing leading to protein expression is the likely mechanism by which RNA helicases contribute to differentiation. DDX17 is involved in mRNA splicing
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
helicase DDX6 colocalizes with TRIM32 in neural stem cells and neurons and increases the activity of Let-7a. The activation of Let-7a depends on the enzyme's helicase activity
-
-
?
ATP + H2O
ADP + phosphate
the ability of RNA helicases to modulate the structure and thus availability of critical RNA molecules for processing leading to protein expression is the likely mechanism by which RNA helicases contribute to differentiation
-
-
?
ATP + H2O
ADP + phosphate
the ability of RNA helicases to modulate the structure and thus availability of critical RNA molecules for processing leading to protein expression is the likely mechanism by which RNA helicases contribute to differentiation. DDX17 is involved in mRNA splicing
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TIP5
DHX9 associates with TIP5 independently of RNA. DHX9 requires the association of TIP5 with mature pRNA to bind to rRNA genes. DHX9 binds to rRNA genes only upon ESC differentiation and its activity guides TIP5 to rRNA genes and establishes heterochromatin
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
DHX9 is expressed in both ESCs and differentiated cells, the latter showing a slight increase at mRNA and protein levels
DDX17 transcripts are abundant in rat brains in early embryonic stages and becomes downregulated in late post-natal and adults, suggesting involvement during neuronal differentiation during development of the central nervous system
-
the 1 kb fragment (5' to the ATG codon) of GRTH gene contains sequences for androgen regulation of its expression in Leydig cells
-
GRTH resides in the nucleus, cytoplasm and chromatoid body of round spermatids
-
the expression of GRTH in testicular cells is differentially regulated by its 5' flanking sequence
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
upon differentiation DHX9 drastically changes its position within the nucleus, and all cells show DHX9 with an exclusive localization within nucleoli and a weak signal in the rest of nucleoplasm
-
-
p68 shuttles between the nucleus and the cytoplasm. The nucleocytoplasmic shuttling of p68 is mediated by two nuclear localization signal and two nuclear exporting signal sequence elements. p68 shuttles via a classical RanGTPase dependent pathway
accumulation of DHX9 in nucleoli and its association with rRNA genes only upon ESC
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
DHX9 is characterized by two copies of a double-stranded RNA binding domain (DSRM) at the N-terminus, a helicase core domain (HrpA) in the central region, and an RGG-rich region at the carboxyl terminus, which confers both RNA and DNA helicase activities
evolution
DDX5 (p68) and DDX17 (p72) belong to the large family of evolutionarily conserved DEAD box RNA helicases. The regulatory activity of DDX5 and DDX17 in transcription is conserved throughout evolution. Possible evolutionary divergence of the insulation process between drosophila and mammals
evolution
DDX5 (p68) and DDX17 (p72) belong to the large family of evolutionarily conserved DEAD box RNA helicases.The regulatory activity of DDX5 and DDX17 in transcription is conserved throughout evolution. Possible evolutionary divergence of the insulation process between drosophila and mammals
malfunction
conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities
malfunction
embryonic stem cells (ESCs) depleted of DHX9 are unable to differentiate, and this phenotype is reverted by the addition of pRNA, whereas providing IGS-rRNA and pRNA mutants deficient for TIP5 binding are not sufficient. Knockdown of TIP5 does not affect DHX9 levels. NIH 3T3 cells depleted of DHX9 show a reduction in rRNA gene silencing
malfunction
mice lacking DDX3X during hematopoiesis show an altered leukocyte composition in bone marrow and spleen and a striking inability to combat infection with Listeria monocytogenes. Mice lacking DDX3X in the hematopoietic system show alterations of bone marrow and splenic cell populations. Alterations in innate immune responses result from decreased effector cell availability and function as well as a sex-dependent impairment of cytokine synthesis. Production of important cytokines such as IL12 or IFNgamma is reduced, DDX3X-deficient macrophages show reduced ability to restrict Listeria monocytogenes growth. Owing to partial redundancy with its close Y chromosomal homologue, DDX3Y, the observed effects differ between mouse sexes. DDX3Y, either alone or together with additional Y-chromosomal genes, partially compensates for the loss of the Ddx3x gene, as homozygous female cells and mice show more severe loss-of-function phenotypes
malfunction
the aberrant expression of DDX5 and/or DDX17 contributes to pathologies such as cancer
metabolism
analysis of lncRNA biogenesis during development and support of a model in which the state of rRNA gene chromatin is part of the regulatory network that controls exit from pluripotency and initiation of differentiation pathways. Critical role of pRNA biogenesis in the establishment of rRNA gene heterochromatin. DHX9 is required for the formation of heterochromatin at rRNA genes through processing of IGS-rRNA into pRNA
metabolism
pivotal and sex-specific role for the heterosomal isoforms of the DEAD box RNA helicase DDX3 in the immune system. Mechanism of DDX3X action, redundancy with DDX3Y, overview
metabolism
protein and noncoding RNA partners of DDX5 and DDX17, DDX5/DDX17 and the regulation of gene insulation, overview. DDX5 and DDX17 can regulate alternative splicing through other mechanisms, via a direct effect on the local folding of their targeted transcripts or via the recruitment of RNA binding cofactors
physiological function
plays a critical role in the mitochondrial RNA surveillance and degradation machinery
physiological function
-
the protein plays a very important role in early organ development and maturation, function of the protein in transcriptional regulation and pre-mRNA splicing
physiological function
-
through its export/transport function as a component of mRNP (mRNAs that associate with ribonuclear particles) GRTH is essential to govern the structure of the chromatoid body in spermatids and to maintain systems that may participate in mRNA storage and their processing during spermatogenesis
physiological function
the balance between stem cell maintenance and neuronal differentiation depends on cell-fate determinants such as TRIM32. TRIM32 associates with the RNA-induced silencing complex and increases the activity of microRNAs such as Let-7a. The RNA helicase DDX6 is involved in microRNA regulation. Helicase DDX6 colocalizes with TRIM32 in neural stem cells and neurons and increases the activity of Let-7a. Enzyme DDX6 is necessary and sufficient for neuronal differentiation and it functions in cooperation with TRIM32
physiological function
RNA helicases DDX5 and DDX17 are multitasking proteins that regulate gene expression in different biological contexts through diverse activities. The enzymes are associated with long noncoding RNAs that are key epigenetic regulators, DDX5 and DDX17 may act through modulating the activity of various ribonucleoprotein complexes that could ensure their targeting to specific chromatin loci. Potential roles of DDX5 and DDX17 in the 3D chromatin organization with impact on gene expression at the transcriptional and post-transcriptional levels. DDX5 may also contribute to cancer development by modulating various signaling pathways. Murine Ddx5 and Ddx17 are essential for the early stages of myoblast or osteoblast differentiation through their interaction with master transcription factors Myod or Runx2, respectively. In both cases, Ddx5 is recruited to Myod and Runx2 responsive promoters, and it enhances their transcriptional activity. During myogenesis, one consequence is the induced expression of myogenic microRNAs, myogenic transcription factors (Myog or Mef2c), as well as muscle specific genes. DDX17 and DDX5 are necessary for repressing the expression of a large subset of neuronal genes in undifferentiated neuroblastoma cells, in cooperation with the REST transcription factor. DDX5 and DDX17 interact and synergize with acetyltransferases CBP (CREB-binding protein) and p300 to activate transcription, such as in the context of SMAD3-mediated transcriptional activation. DDX5 and DDX17 interact with the BRG1 chromatin remodeler. In muscle cells, DDX5 and DDX17 recruit BRG1 to MYOD target genes, increasing the chromatin accessibility for the transcription machinery, which helps coactivate MYOD-dependent transcription. DDX5 may be involved in the control of DNA methylation and/or demethylation of CpG dinucleotides, as it interacts with DNA methyltransferase 3 proteins (DNMT3A and B) as well as with thymine DNA glycosylase (TDG). DDX5 is recruited to chromatin along with both DNMT3A/B and TDG proteins at the beginning of each transcription cycle of an ERalpha-responsive promoter. During myogenic differentiation of mouse C2C12 cells, both RNA helicases and steroid nuclear receptor activator RNA (SRA)coactivate the transcription factor MyoD, and the joint overexpression of SRA and Ddx5 stimulates the MyoD-induced conversion of mouse embryonic fibroblasts in skeletal muscle cells. The SRA lncRNA can act as a multimodal scaffold for several complexes, and it can be dynamically regulated by RNA helicases
physiological function
RNA helicases DDX5 and DDX17 are multitasking proteins that regulate gene expression in different biological contexts through diverse activities. The enzymes are associated with long noncoding RNAs that are key epigenetic regulators, DDX5 and DDX17 may act through modulating the activity of various ribonucleoprotein complexes that could ensure their targeting to specific chromatin loci. Potential roles of DDX5 and DDX17 in the 3D chromatin organization with impact on gene expression at the transcriptional and post-transcriptional levels. Murine Ddx5 and Ddx17 are essential for the early stages of myoblast or osteoblast differentiation through their interaction with master transcription factors Myod or Runx2, respectively. In both cases, Ddx5 is recruited to Myod and Runx2 responsive promoters, and it enhances their transcriptional activity. During myogenesis, one consequence is the induced expression of myogenic microRNAs, myogenic transcription factors (Myog or Mef2c), as well as muscle specific genes. DDX17 and DDX5 are necessary for repressing the expression of a large subset of neuronal genes in undifferentiated neuroblastoma cells, in cooperation with the REST transcription factor. DDX5 and DDX17 interact and synergize with acetyltransferases CBP (CREB-binding protein) and p300 to activate transcription, such as in the context of SMAD3-mediated transcriptional activation. DDX5 and DDX17 interact with the BRG1 chromatin remodeler. In muscle cells, DDX5 and DDX17 recruit BRG1 to MYOD target genes, increasing the chromatin accessibility for the transcription machinery, which helps coactivate MYOD-dependent transcription. During myogenic differentiation of mouse C2C12 cells, both RNA helicases and steroid nuclear receptor activator RNA (SRA) coactivate the transcription factor MyoD, and the joint overexpression of SRA and Ddx5 stimulates the MyoD-induced conversion of mouse embryonic fibroblasts in skeletal muscle cells. The SRA lncRNA can act as a multimodal scaffold for several complexes, and it can be dynamically regulated by RNA helicases
physiological function
rRNA genes undergo chromatin changes during mouse embryonic stem cell (ESC) differentiation. The establishment of heterochromatin at rRNA genes depends on the processing of IGS-rRNA into pRNA. The RNA helicase DHX9 is a regulator of pRNA processing. DHX9 binds to rRNA genes only upon ESC differentiation and its activity guides TIP5 to rRNA genes and establishes heterochromatin. The production of mature pRNA is essential since it guides the repressor TIP5 to rRNA genes. The processing of IGS-rRNA into pRNA is impaired in embryonic stem cells (ESCs) and activated only upon differentiation. IGS-rRNA abolishes the process of guiding of repressor TIP5 to rRNA genes. The RNA helicase DHX9 associates with IGS-rRNA and TIP5. DHX9 is required for the formation of heterochromatin at rRNA genes through processing of IGS-rRNA into pRNA. DHX9 requires the association of TIP5 with mature pRNA to bind to rRNA genes
physiological function
the RNA helicase DDX3X is an essential mediator of innate antimicrobial immunity, essential contribution of a non-RLR DExD/H RNA helicase to innate immunity. Enzyme DDX3 is an interactor of the S/T kinase TBK1 which regulates the production of type I Interferons (IFN-I), contributions of DDX3X to hematopoiesis. DDX3X is critically involved in enhancing the expression of numerous antimicrobial genes. DDX3X may contribute to sex differences in immunity to pathogens and inflammatory disease. Besides its role in the regulation of the TBK1-IRF3 axis, DDX3X controls the NFkappaB signaling pathway and has a profound impact on inflammatory cytokine production. DDX3Y, either alone or together with additional Y-chromosomal genes, partially compensates for the loss of the Ddx3x gene, as homozygous female cells and mice show more severe loss-of-function phenotypes
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). DDX25_MOUSE
484
0
54876
Swiss-Prot
other Location (Reliability: 4)
DDX27_MOUSE
760
0
85939
Swiss-Prot
other Location (Reliability: 1)
DDX28_MOUSE
540
0
59515
Swiss-Prot
Mitochondrion (Reliability: 3)
DDX31_MOUSE
687
0
76913
Swiss-Prot
other Location (Reliability: 4)
DDX3L_MOUSE
660
0
73141
Swiss-Prot
other Location (Reliability: 1)
DDX3X_MOUSE
662
0
73101
Swiss-Prot
other Location (Reliability: 1)
DDX3Y_MOUSE
658
0
73428
Swiss-Prot
other Location (Reliability: 1)
DX39A_MOUSE
427
0
49067
Swiss-Prot
other Location (Reliability: 1)
SMBP2_MOUSE
993
0
109466
Swiss-Prot
other Location (Reliability: 4)
DDX51_MOUSE
639
0
70368
Swiss-Prot
other Location (Reliability: 2)
DDX52_MOUSE
598
0
67474
Swiss-Prot
other Location (Reliability: 3)
DDX54_MOUSE
874
0
97748
Swiss-Prot
other Location (Reliability: 4)
IF4A3_MOUSE
411
0
46840
Swiss-Prot
other Location (Reliability: 5)
YTDC2_MOUSE
1445
0
161092
Swiss-Prot
other Location (Reliability: 3)
AQR_MOUSE
1481
0
170294
Swiss-Prot
other Location (Reliability: 3)
M10L1_MOUSE
1187
0
132792
Swiss-Prot
other Location (Reliability: 3)
MTREX_MOUSE
1040
0
117636
Swiss-Prot
other Location (Reliability: 1)
TDR12_MOUSE
1215
0
137627
Swiss-Prot
other Location (Reliability: 2)
DDX55_MOUSE
600
0
68465
Swiss-Prot
other Location (Reliability: 2)
DDX56_MOUSE
546
0
61212
Swiss-Prot
other Location (Reliability: 1)
DDX58_MOUSE
926
0
105975
Swiss-Prot
other Location (Reliability: 2)
DDX59_MOUSE
619
0
68234
Swiss-Prot
other Location (Reliability: 2)
DDX5_MOUSE
614
0
69290
Swiss-Prot
other Location (Reliability: 3)
U520_MOUSE
2136
0
244547
Swiss-Prot
other Location (Reliability: 2)
DDX41_MOUSE
622
0
69820
Swiss-Prot
other Location (Reliability: 1)
DDX42_MOUSE
929
0
101965
Swiss-Prot
other Location (Reliability: 4)
DDX46_MOUSE
1032
0
117448
Swiss-Prot
other Location (Reliability: 3)
DHX32_MOUSE
744
0
83941
Swiss-Prot
other Location (Reliability: 1)
DHX33_MOUSE
698
0
78347
Swiss-Prot
other Location (Reliability: 4)
DHX34_MOUSE
1145
0
128507
Swiss-Prot
other Location (Reliability: 3)
DHX36_MOUSE
1001
0
113883
Swiss-Prot
other Location (Reliability: 2)
DHX40_MOUSE
779
0
88530
Swiss-Prot
Mitochondrion (Reliability: 4)
FANCJ_MOUSE
1174
0
131360
Swiss-Prot
other Location (Reliability: 3)
FANCM_MOUSE
2021
1
226018
Swiss-Prot
Mitochondrion (Reliability: 3)
DDX47_MOUSE
455
0
50639
Swiss-Prot
other Location (Reliability: 1)
DDX49_MOUSE
480
0
54094
Swiss-Prot
other Location (Reliability: 2)
DDX4_MOUSE
702
0
76470
Swiss-Prot
other Location (Reliability: 1)
DDX50_MOUSE
734
0
82175
Swiss-Prot
other Location (Reliability: 1)
DHX57_MOUSE
1388
0
155762
Swiss-Prot
other Location (Reliability: 2)
DHX58_MOUSE
678
0
76709
Swiss-Prot
other Location (Reliability: 2)
DHX8_MOUSE
1244
0
142572
Swiss-Prot
other Location (Reliability: 5)
DHX9_MOUSE
1380
0
149475
Swiss-Prot
other Location (Reliability: 2)
MOV10_MOUSE
1004
0
113583
Swiss-Prot
other Location (Reliability: 5)
DDX20_MOUSE
825
0
91710
Swiss-Prot
other Location (Reliability: 2)
DDX21_MOUSE
851
0
93551
Swiss-Prot
other Location (Reliability: 2)
DDX24_MOUSE
857
0
96429
Swiss-Prot
other Location (Reliability: 2)
G3BP1_MOUSE
465
0
51829
Swiss-Prot
other Location (Reliability: 2)
IF4A1_MOUSE
406
0
46154
Swiss-Prot
other Location (Reliability: 2)
IF4A2_MOUSE
407
0
46402
Swiss-Prot
other Location (Reliability: 1)
DDX6_MOUSE
483
0
54192
Swiss-Prot
other Location (Reliability: 2)
DHX15_MOUSE
795
0
91007
Swiss-Prot
other Location (Reliability: 1)
DHX29_MOUSE
1365
0
153975
Swiss-Prot
Mitochondrion (Reliability: 3)
DHX30_MOUSE
1217
0
136668
Swiss-Prot
other Location (Reliability: 2)
TDRD9_MOUSE
1383
0
155980
Swiss-Prot
other Location (Reliability: 2)
DDX10_MOUSE
875
0
100739
Swiss-Prot
other Location (Reliability: 3)
DX39B_MOUSE
428
0
49035
Swiss-Prot
other Location (Reliability: 1)
SUV3_MOUSE
779
0
87005
Swiss-Prot
Mitochondrion (Reliability: 1)
DD19A_MOUSE
478
0
53933
Swiss-Prot
other Location (Reliability: 1)
DDX17_MOUSE
650
0
72399
Swiss-Prot
other Location (Reliability: 1)
DDX18_MOUSE
660
0
74181
Swiss-Prot
Mitochondrion (Reliability: 3)
DDX1_MOUSE
740
0
82500
Swiss-Prot
other Location (Reliability: 3)
IFIH1_MOUSE
1025
0
115971
Swiss-Prot
other Location (Reliability: 3)
RENT1_MOUSE
1124
0
123967
Swiss-Prot
other Location (Reliability: 1)
Q5F2A7_MOUSE
406
0
46154
TrEMBL
other Location (Reliability: 2)
Q3TQX5_MOUSE
662
0
73101
TrEMBL
other Location (Reliability: 1)
Q80TP6_MOUSE
1041
0
116841
TrEMBL
other Location (Reliability: 2)
B1ARB9_MOUSE
182
0
20284
TrEMBL
other Location (Reliability: 3)
A7YY79_MOUSE
925
0
105786
TrEMBL
other Location (Reliability: 2)
Q3TIE4_MOUSE
427
0
49053
TrEMBL
other Location (Reliability: 1)
G3X8X0_MOUSE
1044
0
119072
TrEMBL
other Location (Reliability: 2)
Q8BQD9_MOUSE
354
0
38685
TrEMBL
other Location (Reliability: 2)
Q497W9_MOUSE
703
0
79971
TrEMBL
other Location (Reliability: 1)
A0A0G2JGL8_MOUSE
1186
0
133028
TrEMBL
Mitochondrion (Reliability: 5)
Q3U605_MOUSE
444
0
51328
TrEMBL
other Location (Reliability: 1)
Q5DTN5_MOUSE
1264
0
144923
TrEMBL
other Location (Reliability: 5)
Q3TUC8_MOUSE
586
0
67162
TrEMBL
other Location (Reliability: 2)
D3Z3E8_MOUSE
1004
0
113599
TrEMBL
other Location (Reliability: 5)
Q922B8_MOUSE
740
0
82613
TrEMBL
other Location (Reliability: 3)
D6RJ11_MOUSE
97
0
10885
TrEMBL
other Location (Reliability: 1)
Q8CD39_MOUSE
874
0
97720
TrEMBL
other Location (Reliability: 4)
Q8R4Z6_MOUSE
825
0
91783
TrEMBL
other Location (Reliability: 2)
A0A0R4J2C3_MOUSE
459
0
51025
TrEMBL
other Location (Reliability: 2)
A1L0V6_MOUSE
924
0
105685
TrEMBL
other Location (Reliability: 3)
A0A0N4SVK2_MOUSE
196
0
21840
TrEMBL
other Location (Reliability: 2)
Q3UXW3_MOUSE
615
0
69226
TrEMBL
other Location (Reliability: 3)
Q6A0A1_MOUSE
1224
0
140032
TrEMBL
other Location (Reliability: 1)
Q3UDX4_MOUSE
648
0
73393
TrEMBL
other Location (Reliability: 1)
Q9CSI0_MOUSE
528
0
60136
TrEMBL
other Location (Reliability: 2)
Q4VBG1_MOUSE
455
0
50639
TrEMBL
other Location (Reliability: 1)
B2RUM8_MOUSE
660
0
74181
TrEMBL
Mitochondrion (Reliability: 3)
Q3T9L0_MOUSE
427
0
49085
TrEMBL
other Location (Reliability: 1)
A1L333_MOUSE
615
0
69280
TrEMBL
other Location (Reliability: 3)
Q3UD86_MOUSE
1004
0
113555
TrEMBL
other Location (Reliability: 5)
S4R1I6_MOUSE
406
0
46073
TrEMBL
other Location (Reliability: 3)
A0A087WPL5_MOUSE
1383
0
149619
TrEMBL
other Location (Reliability: 2)
Q3UYV7_MOUSE
622
0
69139
TrEMBL
other Location (Reliability: 2)
A0A0N4SWC5_MOUSE
180
0
19654
TrEMBL
other Location (Reliability: 1)
Q5SVX2_MOUSE
237
0
26409
TrEMBL
other Location (Reliability: 1)
Q3U484_MOUSE
658
0
73385
TrEMBL
other Location (Reliability: 1)
Q8CD14_MOUSE
909
0
100793
TrEMBL
other Location (Reliability: 4)
Q9CS87_MOUSE
499
0
56580
TrEMBL
other Location (Reliability: 2)
Q8K1G9_MOUSE
679
0
75877
TrEMBL
other Location (Reliability: 2)
Q8BZY3_MOUSE
494
0
55675
TrEMBL
Mitochondrion (Reliability: 3)
A0A1Y7VKX7_MOUSE
791
0
88651
TrEMBL
other Location (Reliability: 2)
B9EKE9_MOUSE
661
0
73014
TrEMBL
other Location (Reliability: 1)
A0A0N4SVP8_MOUSE
411
0
46959
TrEMBL
Mitochondrion (Reliability: 5)
A0A0N4SVT1_MOUSE
380
0
42339
TrEMBL
other Location (Reliability: 1)
D2CGM4_MOUSE
1025
0
115971
TrEMBL
other Location (Reliability: 3)
E9PV04_MOUSE
411
0
46854
TrEMBL
other Location (Reliability: 5)
Q3U741_MOUSE
652
0
72585
TrEMBL
other Location (Reliability: 1)
Q80X98_MOUSE
1228
0
140623
TrEMBL
other Location (Reliability: 1)
A0A0N4SVM5_MOUSE
266
0
30008
TrEMBL
other Location (Reliability: 5)
F8WHR6_MOUSE
1028
0
117006
TrEMBL
other Location (Reliability: 3)
Q3TFC0_MOUSE
1004
0
113599
TrEMBL
other Location (Reliability: 5)
A0A0G2JG10_MOUSE
608
0
68578
TrEMBL
other Location (Reliability: 1)
Q8K2L4_MOUSE
689
0
75855
TrEMBL
other Location (Reliability: 1)
Q5BKP5_MOUSE
734
0
82176
TrEMBL
other Location (Reliability: 1)
Q8R3C7_MOUSE
479
0
53894
TrEMBL
other Location (Reliability: 1)
B2RQS6_MOUSE
1000
0
113840
TrEMBL
other Location (Reliability: 2)
F8WJA0_MOUSE
903
0
101376
TrEMBL
other Location (Reliability: 4)
D6RHT5_MOUSE
208
0
23204
TrEMBL
other Location (Reliability: 1)
Q3V1Z7_MOUSE
740
0
82501
TrEMBL
other Location (Reliability: 3)
Q3UQQ4_MOUSE
1244
0
142556
TrEMBL
other Location (Reliability: 5)
O35662_MOUSE
169
0
18860
TrEMBL
other Location (Reliability: 3)
Q921Y1_MOUSE
1044
0
119088
TrEMBL
other Location (Reliability: 2)
H3BLJ6_MOUSE
310
0
33953
TrEMBL
other Location (Reliability: 1)
A2AFK7_MOUSE
299
0
33958
TrEMBL
other Location (Reliability: 5)
A0A1W2P700_MOUSE
134
0
14935
TrEMBL
other Location (Reliability: 1)
B1ARC0_MOUSE
167
0
18650
TrEMBL
other Location (Reliability: 3)
Q3TFG3_MOUSE
406
0
46182
TrEMBL
other Location (Reliability: 2)
Q3ULC7_MOUSE
851
0
93595
TrEMBL
other Location (Reliability: 2)
Q5SVX3_MOUSE
546
0
61212
TrEMBL
other Location (Reliability: 1)
Q8BK66_MOUSE
546
0
61180
TrEMBL
other Location (Reliability: 1)
E9PW39_MOUSE
1077
0
120970
TrEMBL
Mitochondrion (Reliability: 5)
Q3TLL6_MOUSE
364
0
41491
TrEMBL
other Location (Reliability: 2)
A0A0G2JG82_MOUSE
135
0
15344
TrEMBL
other Location (Reliability: 2)
Q6PCP0_MOUSE
851
0
93532
TrEMBL
other Location (Reliability: 2)
D3YVL0_MOUSE
1077
0
120986
TrEMBL
Mitochondrion (Reliability: 5)
A0A338P6X5_MOUSE
322
0
36711
TrEMBL
other Location (Reliability: 1)
A0A0G2JE88_MOUSE
139
0
14519
TrEMBL
other Location (Reliability: 2)
Q3TGK7_MOUSE
406
0
46140
TrEMBL
other Location (Reliability: 2)
Q6IQZ1_MOUSE
641
0
72271
TrEMBL
other Location (Reliability: 2)
Q3U7R4_MOUSE
857
0
96331
TrEMBL
other Location (Reliability: 2)
B9EIA2_MOUSE
660
0
73141
TrEMBL
other Location (Reliability: 1)
Q7TPP4_MOUSE
690
0
77531
TrEMBL
other Location (Reliability: 2)
Q8BTS0_MOUSE
615
0
69266
TrEMBL
other Location (Reliability: 3)
Q3UXC2_MOUSE
406
0
46184
TrEMBL
other Location (Reliability: 2)
Q8CFS2_MOUSE
681
0
77877
TrEMBL
other Location (Reliability: 3)
Q8VDG2_MOUSE
544
0
61342
TrEMBL
other Location (Reliability: 2)
Q4FZL1_MOUSE
405
0
46023
TrEMBL
other Location (Reliability: 2)
A0A1S6GWH2_MOUSE
483
0
54643
TrEMBL
other Location (Reliability: 5)
D2CGM5_MOUSE
1025
0
115980
TrEMBL
other Location (Reliability: 3)
Q810A4_MOUSE
602
0
68707
TrEMBL
other Location (Reliability: 2)
Q3UKJ6_MOUSE
795
0
91007
TrEMBL
other Location (Reliability: 1)
Q3TPL7_MOUSE
427
0
49068
TrEMBL
other Location (Reliability: 1)
Q8BRE3_MOUSE
462
0
53100
TrEMBL
other Location (Reliability: 3)
Q80TX4_MOUSE
1018
0
116109
TrEMBL
other Location (Reliability: 2)
Q3UAC4_MOUSE
622
0
69765
TrEMBL
other Location (Reliability: 1)
Q8BKG1_MOUSE
70
0
7399
TrEMBL
other Location (Reliability: 2)
A0A1S6GWJ4_MOUSE
633
0
71101
TrEMBL
other Location (Reliability: 2)
Q8C751_MOUSE
681
0
77839
TrEMBL
other Location (Reliability: 3)
Q69ZE2_MOUSE
1027
0
115715
TrEMBL
other Location (Reliability: 3)
Q7TMM1_MOUSE
623
0
70849
TrEMBL
other Location (Reliability: 5)
Q3UTV0_MOUSE
309
0
34631
TrEMBL
other Location (Reliability: 2)
Q8BK20_MOUSE
533
0
60699
TrEMBL
other Location (Reliability: 2)
E9QNN1_MOUSE
1384
0
149690
TrEMBL
other Location (Reliability: 2)
D6RJ60_MOUSE
96
0
10798
TrEMBL
other Location (Reliability: 1)
Q5SXR4_MOUSE
304
0
34397
TrEMBL
other Location (Reliability: 2)
E9Q9T6_MOUSE
596
0
68023
TrEMBL
other Location (Reliability: 2)
Q3UR42_MOUSE
735
0
82471
TrEMBL
other Location (Reliability: 2)
Q3TU25_MOUSE
418
0
47385
TrEMBL
other Location (Reliability: 1)
D3Z6P9_MOUSE
646
0
72271
TrEMBL
Mitochondrion (Reliability: 3)
Q3TB18_MOUSE
324
0
35987
TrEMBL
other Location (Reliability: 1)
Q3TLR1_MOUSE
792
0
88723
TrEMBL
other Location (Reliability: 2)
B7ZWF1_MOUSE
661
0
73072
TrEMBL
other Location (Reliability: 2)
Q5U222_MOUSE
648
0
72917
TrEMBL
Mitochondrion (Reliability: 5)
Q8R4Z5_MOUSE
505
0
56349
TrEMBL
other Location (Reliability: 2)
Q3TI21_MOUSE
622
0
69790
TrEMBL
other Location (Reliability: 1)
Q3U837_MOUSE
427
0
49089
TrEMBL
other Location (Reliability: 1)
A2A4N9_MOUSE
1174
0
134360
TrEMBL
other Location (Reliability: 5)
A2ACQ1_MOUSE
703
0
78740
TrEMBL
other Location (Reliability: 2)
Q52KC1_MOUSE
407
0
46402
TrEMBL
other Location (Reliability: 1)
Q3TKX4_MOUSE
595
0
67130
TrEMBL
other Location (Reliability: 3)
Q3U606_MOUSE
672
0
75796
TrEMBL
other Location (Reliability: 2)
Q8BRU5_MOUSE
669
0
74591
TrEMBL
other Location (Reliability: 1)
Q3TFE5_MOUSE
795
0
91023
TrEMBL
other Location (Reliability: 1)
Q3V086_MOUSE
728
0
79283
TrEMBL
other Location (Reliability: 1)
Q8BTU6_MOUSE
362
0
41290
TrEMBL
other Location (Reliability: 1)
F6QKD2_MOUSE
360
0
40025
TrEMBL
other Location (Reliability: 1)
D3Z0M9_MOUSE
819
0
95495
TrEMBL
other Location (Reliability: 1)
Q3V0Z8_MOUSE
690
0
76758
TrEMBL
other Location (Reliability: 2)
Q3UVN0_MOUSE
427
0
49083
TrEMBL
other Location (Reliability: 1)
Q3TSJ4_MOUSE
225
0
24870
TrEMBL
other Location (Reliability: 2)
Q78WR5_MOUSE
236
0
26227
TrEMBL
other Location (Reliability: 2)
Q9CWZ5_MOUSE
832
0
91664
TrEMBL
other Location (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
helicase activity of DDX5 is regulated by phosphorylation and calmodulin binding
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
trageting of the enzyme DDX6 with shRNA in N2a cells. Construction of a mutant DDX6 that contains a point mutation within the DEAD box motif II of DDX6 and thus lacks helicase activity, but is still able to coimmunoprecipitate TRIM32
additional information
generation of Ddx3xfl/fl mice, analysis of DDX3X and DDX3Y activity in fibroblasts from gene-targeted mice. Mouse embryonic fibroblasts (MEFs) derived from female Ddx3xfl/fl CreERT2 mice are treated with 4-OHT to delete Ddx3x. Innate immunity of DDX3X-deficient cells and of Ddx3xfl/y Vav-iCre mice is analyzed, overview. Mice lacking DDX3X in hematopoietic cells have reduced numbers of lymphocytes and natural killer cells. Mice lacking DDX3X in the hematopoietic system produce reduced amounts of serum IL-12 and IFNgamma after Listeria monocytogenes infection compromising the immune response of macrophages
additional information
plasmids and siRNAs are transfected in HEK-293T cells using calcium phosphate protocol. NIH 3T3 mouse fibroblasts, U2OS, and murine embryonic stem cells are transfected with the siRNA, siRNA-mediated depletions and synthetic RNA levels are monitored by quantitative RT-PCR expression analysis 3-4 days post transfection
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression of the DEADbox mitif II mutant enzyme in N2a cells leading to reduced Let-7a activity
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
furing development, expression of Supv3L1 begins at the blastocyst stage, becomes widespread and strong in all fetal tissues and cell types, and continues during postnatal growth. Supv3L1 is an developmentally regulated gene, which continues to be expressed in all mature tissues, particularly the rapidly proliferating cells of testes, but also in the brain and sensory organs
GRTH gene expression is transcriptionally upregulated by human chorionic gonadotropin (hCG) via second messenger (cAMP) and androgen in Leydig cells. The expression of GRTH in testicular cells is differentially regulated by its 5' flanking sequence. The 1 kb fragment of GRTH gene (5' to the ATG codon) contains sequences for androgen regulation of its expression in Leydig cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
DDX4 can serve as a useful and highly specific biomarker for the diagnosis of germ cell tumors
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Abdelhaleem, M.
RNA helicases: regulators of differentiation
Clin. Biochem.
38
499-503
2005
Homo sapiens (O00571), Homo sapiens (O15523), Homo sapiens (Q9NQI0), Homo sapiens (Q9UHL0), Homo sapiens, Mus musculus (Q501J6), Mus musculus (Q61656), Mus musculus (Q9QY15), Rattus norvegicus (Q9QY16)
Sato, H.; Tsai-Morris, C.H.; Dufau, M.L.
Relevance of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) in the structural integrity of the chromatoid body during spermatogenesis
Biochim. Biophys. Acta
1803
534-543
2010
Mus musculus
Wang, H.; Gao, X.; Huang, Y.; Yang, J.; Liu, Z.R.
P68 RNA helicase is a nucleocytoplasmic shuttling protein
Cell Res.
19
1388-1400
2009
Mus musculus
Tsai-Morris, C.H.; Sheng, Y.; Gutti, R.; Li, J.; Pickel, J.; Dufau, M.L.
Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) gene: cell-specific expression and transcriptional regulation by androgen in transgenic mouse testis
J. Cell. Biochem.
109
1142-1147
2010
Mus musculus
Paul, E.; Kielbasinski, M.; Sedivy, J.M.; Murga-Zamalloa, C.; Khanna, H.; Klysik, J.E.
Widespread expression of the Supv3L1 mitochondrial RNA helicase in the mouse
Transgenic Res.
19
691-701
2010
Mus musculus (Q80YD1), Mus musculus
Nicklas, S.; Okawa, S.; Hillje, A.L.; Gonzalez-Cano, L.; Del Sol, A.; Schwamborn, J.C.
The RNA helicase DDX6 regulates cell-fate specification in neural stem cells via miRNAs
Nucleic Acids Res.
43
2638-2654
2015
Mus musculus (P54823)
Giraud, G.; Terrone, S.; Bourgeois, C.F.
Functions of DEAD box RNA helicases DDX5 and DDX17 in chromatin organization and transcriptional regulation
BMB Rep.
51
613-622
2018
Drosophila melanogaster, Drosophila melanogaster (P19109), Homo sapiens (P17844), Homo sapiens (Q92841), Mus musculus (Q501J6), Mus musculus (Q61656)
Leone, S.; Baer, D.; Slabber, C.F.; Dalcher, D.; Santoro, R.
The RNA helicase DHX9 establishes nucleolar heterochromatin, and this activity is required for embryonic stem cell differentiation
EMBO Rep.
18
1248-1262
2017
Mus musculus (O70133)
Szappanos, D.; Tschismarov, R.; Perlot, T.; Westermayer, S.; Fischer, K.; Platanitis, E.; Kallinger, F.; Novatchkova, M.; Lassnig, C.; Mueller, M.; Sexl, V.; Bennett, K.L.; Foong-Sobis, M.; Penninger, J.M.; Decker, T.
The RNA helicase DDX3X is an essential mediator of innate antimicrobial immunity
PLoS Pathog.
14
e1007397
2018
Mus musculus (Q62167)
EXTERNAL LINKS (specific for EC-Number 3.6.4.13)
Transporter Classification Database (TCDB): 1.I.1.1.3, 1.G.3.1.7, 3.A.18.1.1, 1.A.53.1.5, 1.A.85.1.6, 1.A.53.1.7, 1.G.3.1.2, 1.G.3.1.1, 3.A.22.1.1, 1.A.53.1.11, 1.A.53.1.6
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Release 2023.1 (January 2023)
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