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Information on EC 3.6.1.6 - nucleoside diphosphate phosphatase and Organism(s) Homo sapiens

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IUBMB Comments
The enzyme, which appears to be limited to metazoa, acts on multiple nucleoside diphosphates as well as on D-ribose 5-diphosphate. Specificity depends on species and isoform.
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This record set is specific for:
Homo sapiens
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
tppase, nucleoside diphosphatase, ndpase, inosine diphosphatase, idpase, ntpdase5, ntpdase6, nucleoside triphosphate diphosphohydrolase-1, e-ntpdase1, sa1684, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
adenosine diphosphatase
-
-
-
-
adenosinepyrophosphatase
-
-
-
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ADPase
-
-
-
-
CDPase
-
-
-
-
ER-UDPase
-
-
-
-
GDPase
-
-
-
-
guanosine 5'-diphosphatase
-
-
-
-
guanosine diphosphatase
-
-
-
-
IDPase
-
-
-
-
inosine 5'-diphosphatase
-
-
-
-
inosine diphosphatase
-
-
-
-
Lysosomal apyrase-like protein of 70 kDa
-
-
-
-
NDPase
-
-
-
-
NTPDase4
-
-
-
-
NTPDase5
-
-
-
-
NTPDase6
NTPDase6 (CD39L2) nucleotidase
-
nucleoside 5'-diphosphatase
-
-
-
-
Nucleoside diphosphatase
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-
-
-
nucleoside diphosphate phosphatase
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-
-
-
nucleoside diphosphate phosphohydrolase
-
-
-
-
Proto-oncogene cph
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-
-
-
thiaminpyrophosphatase
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-
-
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TPPase
-
-
-
-
type B nucleoside diphosphatase
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-
-
-
type L nucleoside diphosphatase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
nucleoside-diphosphate phosphohydrolase
The enzyme, which appears to be limited to metazoa, acts on multiple nucleoside diphosphates as well as on D-ribose 5-diphosphate. Specificity depends on species and isoform.
CAS REGISTRY NUMBER
COMMENTARY hide
9027-69-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + H2O
AMP + phosphate
show the reaction diagram
24.4% of the specific activity with GDP
-
-
?
CDP + H2O
CMP + phosphate
show the reaction diagram
12.9% of the specific activity with GDP
-
-
?
CTP + H2O
CDP + phosphate
show the reaction diagram
3.8% of the specific activity with GDP
-
-
?
GDP + H2O
GMP + phosphate
show the reaction diagram
best substrate
-
-
?
GTP + H2O
GDP + phosphate
show the reaction diagram
4.4% of the specific activity with GDP
-
-
?
IDP + H2O
IMP + phosphate
show the reaction diagram
55.5% of the specific activity with GDP
-
-
?
ITP + H2O
IDP + phosphate
show the reaction diagram
6% of the specific activity with GDP
-
-
?
UDP + H2O
UMP + phosphate
show the reaction diagram
19.8% of the specific activity with GDP
-
-
?
UTP + H2O
UDP + phosphate
show the reaction diagram
1.2% of the specific activity with GDP
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
activates, slightly more effective than Mg2+
Mg2+
activates, slightly less effective than Ca2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetamide
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
GDP
in presence of Ca2+, wild-type and mutant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7 - 7.8
pH 6.7: about 50% of maximal activity with Mg2+ or Ca2+, about 80% of maximal activity with Ca2+, about 70% of maximal activity with Mg2+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ENTP4_HUMAN
616
2
70255
Swiss-Prot
Secretory Pathway (Reliability: 5)
ENTP5_HUMAN
428
2
47517
Swiss-Prot
Secretory Pathway (Reliability: 1)
ENTP6_HUMAN
484
1
53246
Swiss-Prot
other Location (Reliability: 5)
CANT1_HUMAN
401
0
44840
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
x * 43000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 43000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
nevertheless the bacterially expressed refolded enzyme is not glycosylated, it has high specific activity, indicating that glycosylation is not essential for folding or nucleotidase activity of NTPDase6
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C139S
mutant enzyme lacking the single free sulfhydryl is more stable than the wild-type enzyme under refolding conditions, 10-15°C, 2-3 days. The mutant enzyme is less susceptible to oxidative inactivation than the wild-type enzyme
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
mutant enzyme C139S lacking the single free sulfhydryl is more stable than the wild-type enzyme under refolding conditions, 10-15°C, 2-3 days
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
the mutant enzyme C139S is less susceptible to oxidative inactivation than the wild-type enzyme
654678
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli BL21(DE3), refolding of the enzyme from bacterial inclusion bodies
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
refolding of the enzyme from bacterial inclusion bodies. For wild-type enzyme optimal refolding ocurs at 10-15°C over 2-3 days
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ivanenkov, V.V.; Murphy-Piedmonte, D.M.; Kirley, T.L.
Bacterial expression,characterization, and disulfide bond determination of soluble human NTPDase6 (CD39L2) nucleotidase: implications for structure and function
Biochemistry
42
11726-11735
2003
Homo sapiens (O75354), Homo sapiens
Manually annotated by BRENDA team