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Literature summary for 3.6.1.6 extracted from

  • Ivanenkov, V.V.; Murphy-Piedmonte, D.M.; Kirley, T.L.
    Bacterial expression,characterization, and disulfide bond determination of soluble human NTPDase6 (CD39L2) nucleotidase: implications for structure and function (2003), Biochemistry, 42, 11726-11735.
    View publication on PubMed
Search for more literature for 3.6.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3), refolding of the enzyme from bacterial inclusion bodies Homo sapiens

Protein Variants

Protein Variants Comment Organism
C139S mutant enzyme lacking the single free sulfhydryl is more stable than the wild-type enzyme under refolding conditions, 10-15°C, 2-3 days. The mutant enzyme is less susceptible to oxidative inactivation than the wild-type enzyme Homo sapiens

General Stability

General Stability Organism
mutant enzyme C139S lacking the single free sulfhydryl is more stable than the wild-type enzyme under refolding conditions, 10-15°C, 2-3 days Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 GDP in presence of Ca2+, wild-type and mutant enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, slightly more effective than Mg2+ Homo sapiens
Mg2+ activates, slightly less effective than Ca2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O75354
-
-

Oxidation Stability

Oxidation Stability Organism
the mutant enzyme C139S is less susceptible to oxidative inactivation than the wild-type enzyme Homo sapiens

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein nevertheless the bacterially expressed refolded enzyme is not glycosylated, it has high specific activity, indicating that glycosylation is not essential for folding or nucleotidase activity of NTPDase6 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
refolding of the enzyme from bacterial inclusion bodies. For wild-type enzyme optimal refolding ocurs at 10-15°C over 2-3 days Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O 24.4% of the specific activity with GDP Homo sapiens AMP + phosphate
-
 ?
CDP + H2O 12.9% of the specific activity with GDP Homo sapiens CMP + phosphate
-
 ?
CTP + H2O 3.8% of the specific activity with GDP Homo sapiens CDP + phosphate
-
 ?
GDP + H2O best substrate Homo sapiens GMP + phosphate
-
 ?
GTP + H2O 4.4% of the specific activity with GDP Homo sapiens GDP + phosphate
-
 ?
IDP + H2O 55.5% of the specific activity with GDP Homo sapiens IMP + phosphate
-
 ?
ITP + H2O 6% of the specific activity with GDP Homo sapiens IDP + phosphate
-
 ?
additional information no activity with ATP Homo sapiens ?
-
 ?
UDP + H2O 19.8% of the specific activity with GDP Homo sapiens UMP + phosphate
-
 ?
UTP + H2O 1.2% of the specific activity with GDP Homo sapiens UDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
NTPDase6
-
 Homo sapiens
NTPDase6 (CD39L2) nucleotidase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.4
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.7 7.8 pH 6.7: about 50% of maximal activity with Mg2+ or Ca2+, about 80% of maximal activity with Ca2+, about 70% of maximal activity with Mg2+ Homo sapiens