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Information on EC 3.2.1.31 - beta-glucuronidase and Organism(s) Homo sapiens

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Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
beta-glucuronidase, beta-d-glucuronidase, betag, beta-gluc, beta-glucuronidase a, hgusb, exo-beta-glucuronidase, tmgusi, exo-beta-d-glucuronidase, ketodase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Beta-D-glucuronoside glucuronosohydrolase
-
-
-
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Beta-G1
-
-
-
-
beta-Gluc
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beta-glucuronidase
-
-
betaG
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exo-beta-D-glucuronidase
-
-
-
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glucuronidase, beta-glucuronide glucuronohydrolase
-
-
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hGUSB
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ketodase
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-glucuronoside glucuronosohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-45-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1S)-1-(chloromethyl)-3-([5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]carbonyl)-2,3-dihydro-1H-benzo[e]indol-5-yl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + [(1S)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indol-3-yl][5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]methanone
show the reaction diagram
-
-
-
-
?
(1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-{[({2-[({[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy}carbonyl)amino]-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl}oxy)carbonyl]amino}-alpha-D-lyxo-hexopyranoside + H2O
D-glucuronate + (1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-[({[2-({[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl}amino)-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl]oxy}carbonyl)amino]-alpha-D-lyxo-hexopyranoside
show the reaction diagram
-
-
-
-
?
1-O-([4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamoyl)-beta-D-glucuronate + H2O
D-glucuronate + [4-[(10R,11S)-10-[[((2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamic acid
show the reaction diagram
-
-
-
-
?
1-O-[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O
D-glucuronate + (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamic acid
show the reaction diagram
-
-
-
-
?
1-O-[(4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O
D-glucuronate + (4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamic acid
show the reaction diagram
-
-
-
-
?
2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4,7-dimethyl-3,8,13-trioxo-11,13-diphenyl-2,9-dioxa-4,7,12-triazatridec-1-yl]phenyl beta-D-glucopyranosiduronate + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4,7-dimethyl-3,8-dioxo-2,9-dioxa-4,7-diazaundecan-11-oate
show the reaction diagram
-
-
-
-
?
2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate
show the reaction diagram
-
-
-
-
?
4-([[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxy-3-nitrobenzyl (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamate
show the reaction diagram
-
-
-
-
?
4-([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-9-yl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + (4S)-4-ethyl-4-hydroxy-9-[(4-hydroxy-3-nitrobenzyl)oxy]-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronic acid + H2O
4-methylumbelliferone + beta-D-glucuronic acid
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferol + D-glucuronate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
show the reaction diagram
-
-
-
-
?
4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]-2-nitrophenyl beta-D-glucuronate + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-10-[(S)-(benzoylamino)(phenyl)methyl]-1-(4-hydroxy-3-nitrophenyl)-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate
show the reaction diagram
-
-
-
-
?
4-[([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)methyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxy-3-nitrobenzyl (3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-carboxylate
show the reaction diagram
-
-
-
-
?
4-[([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamoyl]oxy)methyl]phenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxybenzyl [(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamate
show the reaction diagram
-
-
-
-
?
4-[2-[1-(2-[2-[2-(beta-D-glucopyranuronosyloxy)ethoxy]ethoxy]ethyl)-1H-1,2,3-triazol-4-yl]-1-([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)ethyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 2-[2-(2-[4-[2-([[(3beta,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)-2-(4-hydroxy-3-nitrophenyl)ethyl]-1H-1,2,3-triazol-1-yl]ethoxy)ethoxy]ethyl beta-D-glucopyranosiduronic acid
show the reaction diagram
-
-
-
-
?
daidzein 7-O-beta-D-glucuronide + H2O
daidzein + D-glucuronate
show the reaction diagram
-
-
-
-
?
equol 7-O-beta-D-glucuronide + H2O
equol + D-glucuronate
show the reaction diagram
-
-
-
-
?
kaempferol 3-O-beta-D-glucuronide + H2O
kaempferol + D-glucuronate
show the reaction diagram
-
-
-
-
?
N-([[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy]carbonyl)-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-met + H2O
D-glucuronate + N-[[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl]-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide
show the reaction diagram
-
-
-
-
?
phenolphthalein beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
show the reaction diagram
-
-
-
-
?
phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
show the reaction diagram
-
-
-
-
?
quercetin 3-O-beta-D-glucuronide + H2O
quercetin + D-glucuronate
show the reaction diagram
-
-
-
-
?
quercetin 4'-O-beta-D-glucuronide + H2O
quercetin + beta-D-glucuronate
show the reaction diagram
-
-
-
-
?
quercetin 7-O-beta-D-glucuronide + H2O
quercetin + D-glucuronate
show the reaction diagram
-
-
-
-
?
quercetin-4'-O-glucuronide + H2O
quercetin + D-glucuronate
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,1'-(3-methyl-4-phenylthieno[2,3-b]thiophene-2,5-diyl)bis(4,4,4-triethoxybut-2-en-1-one)
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-
ascorbic acid
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-
azide
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50 mM, 70% loss of activity of wild-type enzyme and mutant enzyme E451A. 50 mM-0.5 M, stimulation of mutant enzyme E451A. 1 mM, inhibition of wild-type enzyme and mutant enzyme E451A
Cu2+
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-
D-saccharic acid 1,4-lactone
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-
estradiol 3-glucuronide
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-
fucoxanthin
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mixed-type inhibition
glycyrrhizin
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-
Hg2+
-
reversed by EDTA
inhibitor
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heat stable, competetive, in plasma
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Ni2+
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-
paracetamol glucuronide
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saccharo-1,4-lactone
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complete inhibition of liver enzyme
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
azide
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50 mM-0.5 M, stimulation of mutant enzyme E451A
bovine serum albumin
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activation
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chlorpyrifos-methyl
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diazinon
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dichlorvos
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fenitrothion
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fenthion
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-
Plasma serum
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acivation
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propetamphos
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.25
4-methylumbelliferyl-beta-D-glucuronic acid
pH and temperature not specified in the publication
0.041 - 2.76
4-methylumbelliferyl-beta-D-glucuronide
1.4
4-nitrophenyl-beta-D-glucuronide
-
pH 7.2, 37°C
0.272
daidzein 7-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.266
equol 7-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.025
kaempferol 3-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.13 - 2.9
p-nitrophenyl-beta-D-glucuronide
-
-
0.018 - 3.08
phenolphthalein beta-D-glucuronide
-
dependency on pH
0.048
quercetin 4'-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.237
quercetin 7-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.167
quercetin-3-O-beta-D-glucuronide
-
pH 7.2, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004 - 276
4-methylumbelliferyl-beta-D-glucuronide
0.67
4-nitrophenyl-beta-D-glucuronide
-
pH 7.2, 37°C
1.07
daidzein 7-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.35
equol 7-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.65
kaempferol 3-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.26
quercetin 4'-O-beta-D-glucuronide
-
pH 7.2, 37°C
1.65
quercetin 7-O-beta-D-glucuronide
-
pH 7.2, 37°C
1.9
quercetin-3-O-beta-D-glucuronide
-
pH 7.2, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9 - 1.2
estradiol 3-glucuronide
0.47 - 0.57
glycyrrhizin
1.6 - 2
paracetamol glucuronide
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0009
1,1'-(3-methyl-4-phenylthieno[2,3-b]thiophene-2,5-diyl)bis(4,4,4-triethoxybut-2-en-1-one)
Homo sapiens
-
pH not specified in the publication, 37°C
0.0458
D-saccharic acid 1,4-lactone
Homo sapiens
-
pH not specified in the publication, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31.33
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
substrate 4-methylumbelliferyl-beta-D-glucuronide
4 - 4.5
-
-
4 - 5
-
mutant enzyme E451A
4.5
-
wild-type enzyme
4.6
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
high varability in the expression
Manually annotated by BRENDA team
-
chronic myelogenous
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
recombinant enzyme in BHK cells
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
-
beta-glucuronidase, an acid hydrolase that deconjugates glucuronides, may increase cancer risk
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BGLR_HUMAN
651
1
74732
Swiss-Prot
Secretory Pathway (Reliability: 1)
KLOT_HUMAN
1012
1
116181
Swiss-Prot
Secretory Pathway (Reliability: 4)
B2R6X2_HUMAN
651
1
74706
TrEMBL
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
250000 - 310000
300000
-
SDS-PAGE
390000
-
wild-type enzyme, gel filtration
75000
-
4 * 75000, SDS-PAGE
79000
-
2 * 79000, intracellular human beta-glucuronidase in transfected BHK cells, SDS-PAGE
83000
-
4 * 83000, the enzyme consists of two disulfide-linked dimers, wild-type enzyme and secreted recombinant enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 79000, intracellular human beta-glucuronidase in transfected BHK cells, SDS-PAGE
homotetramer
-
4 * 75000, SDS-PAGE
tetramer
additional information
-
the enzyme is composed of three components of 18000, 64000 and 80000 Da
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
phosphoprotein
-
both the placental 80000 Da and 64000 Da peptides, though not the 18000 Da one, are apparently phosphorylated, whereas the enzyme of leukemic cells is poorly phosphorylated
proteolytic modification
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D207A
-
4.9% of the wild-type activity is expressed in COS cells
E451A
-
0.6% of the wild-type activity is expressed in COS cells, tetrameric enzyme, ratio of turnover number to KM-value is decreased 9100fold as compared to wild-type enzyme. Mutant enzyme E541A is inactivated at a faster rate than the wild-type enzyme, and is completely inactivated within 30 min. 50 mM azide causes 70% loss of activity of wild-type enzyme and mutant enzyme E451A. 50 mM-0.5 M stimulates mutant enzyme E451A. 1 mM inhibits wild-type enzyme and mutant enzyme E451A
E451Q
-
5.9% of the wild-type activity is expressed in COS cells
E515A
-
no activity is expressed in COS cells
E540A
-
no activity is expressed in COS cells, tetrameric enzyme, ratio of turnover number to KM-value is decreased 33000 fold as compared to wild-type enzyme, optimal pH is 5.0 instead of 4.5 for the wild-type enzyme. Mutant enzyme is inactivated at a faster rate than the wild-type enzyme at 68°C
E540D
-
no activity is expressed in COS cells
E540Q
-
0.3% of the wild-type activity is expressed in COS cells
Y504A
-
1.4% of the wild-type activity is expressed in COS cells, tetrameric enzyme, the ratio of turnover number to KM-value is decreased 830fold as compared to wild-type enzyme. Mutant enzyme is as stable as the wild-type enzyme at 68°C
Y504F
-
2.3% of the wild-type activity is expressed in COS cells
Y504H
-
0.6% of the wild-type activity is expressed in COS cells
Y508A
-
no activity is expressed in COS cells
additional information
-
in the mutant enzyme a stop codon is introduced after the CAC/His-637 codon, thereby eliminating 14 amino acids from the C-terminus. Thereby the last Cys at position 644 is eliminated. In the mutant, covalent linkage between two monomers is no longer observed, indicating that Cys44 is involved in intermolecular disulfide-bond formation
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
depending on pH, substrate protects against inactivation
65
-
retains 90% of initial activity after 3 h at 65°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Blue Sepharose FF column chromatography, phenyl-Sepharose high-Sub FF column chromatography, DEAE-Sephacel column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in 3T3 fibroblasts and EJ carcinoma cells
-
expression vectors pMCG-GLUC and pMCG-dGLUC containing cDNA coding for human beta-glucuronidase and a C-terminal-truncated form of beta-glucuronidase respectively are transfected into BHK cells. The stable clones B702 expressing wild-type and B688 expressing mutant beta-glucuronidase. In the mutant enzyme a stop codon is introduced after the CAC/His-637 codon, thereby eliminating 14 amino acids from the C-terminus
-
wild-type and mutant enzymes expressed COS cells and GUSB-deficient fibroblasts from the SV40 late promoter in vector pJC119
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
medicine
nutrition
-
crossover feeding trial in healthy women and men with a diet high in selected citrus fruit, crucifers and soy and a diet devoid of fruits, vegetables, and soy. Participants of the fruit and soy diet display a signifcantly higher beta-glucuronidase activity than those with the basal diet, where the enzyme activity decreased during the diet. Response to the diet does not differ by sex
toxicology
-
measurement of beta-glucuronidase activity has no additive clinical value following a parathion overdose in humans
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gupta, G.S.; Singh, G.P.
Isolation and characterization of the major form of beta-glucuronidase from human seminal plasma
Biochim. Biophys. Acta
748
398-404
1983
Homo sapiens
Manually annotated by BRENDA team
Brot, F.E.; Bell, C.E.; Sly, W.S.
Purification and properties of beta-glucuronidase from human placenta
Biochemistry
17
385-391
1978
Homo sapiens
Manually annotated by BRENDA team
Contractor, S.F.; Oakey, M.
Use of borate buffers for purification of placental beta-glucuronidase on DEAE-cellulose
Anal. Biochem.
78
279-282
1977
Homo sapiens
Manually annotated by BRENDA team
Contractor, S.F.; Shane, B.
Purification and characterization of lysosomal -glucuronidase from human placenta
Biochem. J.
128
11-18
1972
Homo sapiens
Manually annotated by BRENDA team
Gehrmann, M.C.; Opper, M.; Sedlacek, H.H.; Bosslet, K.; Czech, J.
Biochemical properties of recombinant human beta-glucuronidase synthesized in baby hamster kidney cells
Biochem. J.
301
821-828
1994
Homo sapiens
Manually annotated by BRENDA team
Tanaka, J.; Gasa, S.; Sakurada, K.; Miyazaki, T.; Kasai, M.; Makita, A.
Characterization of the subunits and sugar moiety of human placental and leukemic beta-glucuronidase
Biol. Chem. Hoppe-Seyler
373
57-62
1992
Homo sapiens
Manually annotated by BRENDA team
Islam, M.R.; Tomatsu, S.; Shah, G.N.; Grubb, J.H.; Jain, S.; Sly, W.S.
Active site residue of human beta-glucuronidase. Evidence for Glu540 as the nucleophile and Glu451 as the acid-base residue
J. Biol. Chem.
274
23451-23455
1999
Homo sapiens
Manually annotated by BRENDA team
Sperker, B.; Mrdter, T.E.; Schick, M.; Eckhardt, K.; Bosslet, K.; Kroemer, H.K.
Interindividual variability in expression and activity of human beta-glucuronidase in liver and kidney: consequences for drug metabolism
J. Pharmacol. Exp. Ther.
281
914-920
1997
Homo sapiens
Manually annotated by BRENDA team
Chen, K.C.; Cheng, T.L.; Leu, Y.L.; Prijovich, Z.M.; Chuang, C.H.; Chen, B.M.; Roffler, S.R.
Membrane-localized activation of glucuronide prodrugs by beta-glucuronidase enzymes
Cancer Gene Ther.
14
187-200
2007
Escherichia coli, Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Grubb, J.H.; Vogler, C.; Levy, B.; Galvin, N.; Tan, Y.; Sly, W.S.
Chemically modified beta-glucuronidase crosses blood-brain barrier and clears neuronal storage in murine mucopolysaccharidosis VII
Proc. Natl. Acad. Sci. USA
105
2616-2621
2008
Homo sapiens
Manually annotated by BRENDA team
Maruti, S.S.; Chang, J.L.; Prunty, J.A.; Bigler, J.; Schwarz, Y.; Li, S.S.; Li, L.; King, I.B.; Potter, J.D.; Lampe, J.W.
Serum beta-glucuronidase activity in response to fruit and vegetable supplementation: a controlled feeding study
Cancer Epidemiol. Biomarkers Prev.
17
1808-1812
2008
Homo sapiens
Manually annotated by BRENDA team
O'Leary, K.A.; Day, A.J.; Needs, P.W.; Sly, W.S.; O'Brien, N.M.; Williamson, G.
Flavonoid glucuronides are substrates for human liver beta-glucuronidase
FEBS Lett.
503
103-106
2001
Homo sapiens
Manually annotated by BRENDA team
Sabbe, M.B.; Desruelles, D.; Lissens, W.
Is beta-glucuronidase a clinical useful biomarker for an acute organophosphorus poisoning?
Hum. Exp. Toxicol.
27
431-433
2008
Homo sapiens
Manually annotated by BRENDA team
Maruti, S.S.; Li, L.; Chang, J.L.; Prunty, J.; Schwarz, Y.; Li, S.S.; King, I.B.; Potter, J.D.; Lampe, J.W.
Dietary and demographic correlates of serum beta-glucuronidase activity
Nutr. Cancer
62
208-219
2010
Homo sapiens
Manually annotated by BRENDA team
Ueyama, J.; Satoh, T.; Kondo, T.; Takagi, K.; Shibata, E.; Goto, M.; Kimata, A.; Saito, I.; Hasegawa, T.; Wakusawa, S.; Kamijima, M.
beta-Glucuronidase activity is a sensitive biomarker to assess low-level organophosphorus insecticide exposure
Toxicol. Lett.
193
115-119
2010
Homo sapiens
Manually annotated by BRENDA team
Tranoy-Opalinski, I.; Legigan, T.; Barat, R.; Clarhaut, J.; Thomas, M.; Renoux, B.; Papot, S.
beta-Glucuronidase-responsive prodrugs for selective cancer chemotherapy: an update
Eur. J. Med. Chem.
74
302-313
2014
Homo sapiens
Manually annotated by BRENDA team
Kawee-Ai, A.; Kim, S.M.
Application of microalgal fucoxanthin for the reduction of colon cancer risk: inhibitory activity of fucoxanthin against beta-glucuronidase and DLD-1 cancer cells
Nat. Prod. Commun.
9
921-924
2014
Escherichia coli, Homo sapiens
Manually annotated by BRENDA team
Mabkhot, Y.N.; Barakat, A.; Yousuf, S.; Choudhary, M.I.; Frey, W.; Ben Hadda, T.; Mubarak, M.S.
Substituted thieno[2,3-b]thiophenes and related congeners: synthesis, beta-glucuronidase inhibition activity, crystal structure, and POM analyses
Bioorg. Med. Chem.
22
6715-6725
2014
Homo sapiens
Manually annotated by BRENDA team
Panagiotopoulou, E.C.; Fouzas, S.; Douros, K.; Triantaphyllidou, I.E.; Malavaki, C.; Priftis, K.N.; Karamanos, N.K.; Anthracopoulos, M.B.
Increased beta-glucuronidase activity in bronchoalveolar lavage fluid of children with bacterial lung infection: A case-control study
Respirology
20
1248-1254
2015
Homo sapiens
Manually annotated by BRENDA team
Ce, J.; Rodrigues, M.T.; Kaefer, E.T.; da Costa Moraes, V.; Coelho, J.C.
Beta-glucuronidase activity in dried blood spots Reduced technique with biochemical parameters determined
Clin. Biochem.
50
1243-1248
2017
Homo sapiens (P08236)
Manually annotated by BRENDA team