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Information on EC 2.8.2.14 - bile-salt sulfotransferase and Organism(s) Homo sapiens

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.14 bile-salt sulfotransferase
IUBMB Comments
The formation of sulfate esters of bile acids is an essential step in the prevention of toxicity by monohydroxy bile acids in many species . This enzyme is both a bile salt and a 3-hydroxysteroid sulfotransferase. In addition to the 5beta-bile acid glycolithocholate, deoxycholate, 3beta-hydroxy-5-cholenoate and dehydroepiandrosterone (3beta-hydroxyandrost-5-en-17-one) also act as substrates [see also EC 2.8.2.2 (alcohol sulfotransferase) and EC 2.8.2.34 (glycochenodeoxycholate sulfotransferase)]. May be identical to EC 2.8.2.2 .
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Homo sapiens
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
hydroxysteroid sulfotransferase, dehydroepiandrosterone sulfotransferase, sulfotransferase 2a1, sult2a8, bss i, bast i, glycolithocholate sulfotransferase, ml-stl, bst 1, bss ii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3'-phosphoadenylyl-sulfate:taurolithocholate sulfotransferase
-
-
-
-
BAST I
-
-
-
-
bile acid sulfotransferase I
-
-
-
-
bile acid:3'-phosphoadenosine-5'-phosphosulfate sulfotransferase
-
-
-
-
bile salt:3'phosphoadenosine-5'-phosphosulfate:sulfotransferase
-
-
-
-
cytosolic sulfotransferase
-
dehydroepiandrosterone sulfotransferase
-
-
dehydroepiandrosterone-SULT
-
-
DHEA-SULT
-
-
glycolithocholate sulfotransferase
-
-
-
-
hydroxysteroid sulfotransferase
-
-
steroid-/bile acid-sulfotransferase
-
steroid/bile acid-sulfotransferase
-
-
-
-
sulfotransferase 2A1
-
SULT2A1
additional information
see also EC 2.8.2.2
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:glycolithocholate sulfotransferase
The formation of sulfate esters of bile acids is an essential step in the prevention of toxicity by monohydroxy bile acids in many species [3]. This enzyme is both a bile salt and a 3-hydroxysteroid sulfotransferase. In addition to the 5beta-bile acid glycolithocholate, deoxycholate, 3beta-hydroxy-5-cholenoate and dehydroepiandrosterone (3beta-hydroxyandrost-5-en-17-one) also act as substrates [see also EC 2.8.2.2 (alcohol sulfotransferase) and EC 2.8.2.34 (glycochenodeoxycholate sulfotransferase)]. May be identical to EC 2.8.2.2 [3].
CAS REGISTRY NUMBER
COMMENTARY hide
65802-92-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenosine-5'-phosphosulfate + oestrone
adenosine 3',5'-bisphosphate + estrone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + (S)-dehydroepiandrosterone
adenosine 3',5'-bisphosphate + (S)-dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 3alpha-7alpha-chenodeoxycholic acid
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + glycolithocholate
adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + glycolithocholic acid
adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + lithocholic acid
adenosine 3',5'-bisphosphate + lithocholate 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + taurolithocholic acid
adenosine 3',5'-bisphosphate + taurolithocholate 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + 3alpha,7alpha,12alpha-cholic acid
adenosine 3',5'-bisphosphate + 3alpha,7alpha,12alpha-cholic acid sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + 5-cholenic acid-3beta-ol
adenosine 3',5'-bisphosphate + 5-cholenic acid-3-beta-ol sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + allocholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + chenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl-sulfate + chenodeoxycholate
adenosine 3',5'-bisphosphate + chenodeoxycholate 7-sulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + cholic acid
adenosine 3',5'-bisphosphate + cholic acid sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + deoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl-sulfate + deoxycholate
adenosine 3',5'-bisphosphate + deoxycholate 12-sulfate
show the reaction diagram
-
sulfation at the 12-OH position
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated chenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated deoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated lithocholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated ursodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + glycochenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + glycochenodeoxycholate
adenosine 3',5'-bisphosphate + glycochenodeoxycholate monosulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + glycolithocholate
adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + hyodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + lithocholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl-sulfate + lithocholate
adenosine 3',5'-bisphosphate + lithocholate 3-sulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + petromyzonol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + phenol
adenosine 3',5'-bisphosphate + phenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated chenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated deoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated lithocholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated ursodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + taurochenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + ursodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + chenodeoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + cholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
lowest affinity to sulfation by SULT2A1
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + deoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + lithocholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
highest affinity to sulfation by SULT2A1
-
-
?
3'-phosphoadenylylsulfate + ursodeoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + glycolithocholate
adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
show the reaction diagram
the enzyme mediates catalytic conversion of hydroxysteroids, bile acids, and certain clinical drugs to water-soluble sulfated metabolites
-
-
?
3'-phosphoadenylyl sulfate + glycolithocholic acid
adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + lithocholic acid
adenosine 3',5'-bisphosphate + lithocholate 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + taurolithocholic acid
adenosine 3',5'-bisphosphate + taurolithocholate 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chenodeoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + cholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
lowest affinity to sulfation by SULT2A1
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
show the reaction diagram
-
transcriptional regulation of dehydroepiandrosterone sulfotransferase (SULT2A1) by estrogen-related receptor alpha
-
-
?
3'-phosphoadenylylsulfate + deoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + lithocholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
highest affinity to sulfation by SULT2A1
-
-
?
3'-phosphoadenylylsulfate + ursodeoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
additional information
?
-
-
is a target for transcriptional induction by the vitamin D receptor
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-Dichloro-4-nitrophenol
non-specific SULT inhibitor
3-ketolithocholate
-
-
4'-hydroxytoremifene
-
-
4-hydroxytoremifene
-
-
5,7-dichlorokynurenic acid
-
7-methoxylasofoxifene
-
-
acolbifene
-
adenosine-3',5'-diphosphate
-
-
arzoxifene
full mixed inhibition
bazedoxifene
full noncompetitive inhibition
bazedoxifene N-oxide
-
-
cis-4-(1,2,3,4-tetrahydro-6-methoxy-2-phenyl-1-naphthalenyl)phenol
-
-
clomifene
full competitive inhibition
des(1-azepanyl)ethylbazedoxifene
-
-
droloxifene
partial mixed inhibition
gavestinel
i.e. 4,6-dichloro-3-[(1E)-3-oxo-3-(phenylamino)-1-propenyl]-1H-indole-2-carboxylic acid sodium salt
glycolithocholate
-
-
glycolithocholic acid
substrate inhibition
Kynurenic acid
-
L689,560
i.e. trans-2-carboxy-5,7-dichloro-4-phenylaminocarboxylamino-1,2,3,4-tetrahydroquinoline
L701,324
i.e. 7-chloro-4-hydroxy-3(3-phenoxy)phenyl-2(H)-quinolinone
lasofoxifene
full noncompetitive inhibition
lithocholic acid
substrate inhibition
Mefenamic acid
-
N-desmethyl-4-hydroxytoremifene
-
-
N-desmethyltoremifene
-
-
nafoxidine
-
ospemifene
-
p-chloromercuribenzoate
-
-
raloxifene
raloxifene is linked to the development of cholestasis, and it inhibits sulfotransferase 2A1 (SULT2A1)-catalyzed dehydroepiandrosterone (DHEA) sulfonation, full noncompetitive inhibition
tamoxifen
tamoxifen is linked to the development of cholestasis, and it inhibits sulfotransferase 2A1 (SULT2A1)-catalyzed dehydroepiandrosterone (DHEA) sulfonation
taurolithocholic acid
substrate inhibition
toremifene
-
Zn2+
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
constitutive androstane receptor
3.5fold induction of SULT2A1 mRNAs in human cell lines of enterohepatic origin, such as Caco-2 intestinal cells and HepG2 hepatoma cells transfected with the CAR plasmid
-
pregnane X receptor
1.7fold induction of SULT2A1 mRNAs in human cell lines of enterohepatic origin, such as Caco-2 intestinal cells and HepG2 hepatoma cells transfected with the PXR plasmid
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007 - 0.0115
3'-phosphoadenylylsulfate
0.071
3alpha,7alpha,12alpha-cholic acid
-
pH 6.5, 37°C
0.025
3alpha-7alpha-chenodeoxycholic acid
-
pH 6.5, 37°C
0.0024
5-cholenic acid-3beta-ol
-
pH 6.5, 37°C
0.01566 - 0.0423
chenodeoxycholate
0.01566
chenodeoxycholic acid
apparent value, at pH 5.5 and 7.4, at 37°C
0.0014 - 0.00383
dehydroepiandrosterone
0.00886
deoxycholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.00886
deoxycholic acid
apparent value, at pH 5.5 and 7.4, at 37°C
0.04899
glycine conjugated chenodeoxycholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.05459
glycine conjugated deoxycholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.00192
glycine conjugated lithocholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.04957
glycine conjugated ursodeoxycholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.03673
glycochenodeoxycholate
-
in 50 mM Mops buffer at pH 7.0, at 37°C
0.0018 - 0.0033
glycolithocholate
0.00048 - 0.00099
glycolithocholic acid
0.002 - 0.00836
lithocholate
0.00034 - 0.00202
lithocholic acid
0.04938
Petromyzonol
-
in 50 mM Mops buffer at pH 7.0, at 37°C
0.04509
taurine conjugated chenodeoxycholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.01836
taurine conjugated deoxycholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.00186
taurine conjugated lithocholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.00993
taurine conjugated ursodeoxycholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.0004
taurolithocholic acid
liver cytosol, pH 7.4, 37°C
0.01286
ursodeoxycholate
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.01286
Ursodeoxycholic acid
apparent value, at pH 5.5 and 7.4, at 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0006
3-ketolithocholate
-
pH 6.5, 37°C
0.0002
adenosine-3',5'-diphosphate
-
pH 6.5, 37°C
0.0008
arzoxifene
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid, mixed inhibition
0.0002
bazedoxifene
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid, noncompetitive inhibition
0.0058
clomifene
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid, competitive inhibition
0.0046
droloxifene
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid, mixed inhibition
0.0022
glycolithocholate
-
pH 6.5, 37°C
0.0096 - 0.0123
glycolithocholic acid
0.0008
lasofoxifene
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid, noncompetitive inhibition
0.0057 - 0.0391
lithocholic acid
0.0005
raloxifene
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid, noncompetitive inhibition
0.0081
taurolithocholic acid
liver cytosol, pH 7.4, 37°C
additional information
additional information
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0018
4'-hydroxytoremifene
Homo sapiens
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid
-
0.0044
4-hydroxytoremifene
Homo sapiens
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid
-
0.0029
7-methoxylasofoxifene
Homo sapiens
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid
-
0.007 - 0.0129
acolbifene
0.00008 - 0.00029
arzoxifene
0.00013 - 0.00029
bazedoxifene
0.00006
bazedoxifene N-oxide
Homo sapiens
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid
-
0.0014
cis-4-(1,2,3,4-tetrahydro-6-methoxy-2-phenyl-1-naphthalenyl)phenol
Homo sapiens
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid
-
0.0015 - 0.0036
clomifene
0.00016
des(1-azepanyl)ethylbazedoxifene
Homo sapiens
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid
-
0.00075 - 0.0015
droloxifene
0.00025 - 0.001
lasofoxifene
0.0129
N-desmethyl-4-hydroxytoremifene
Homo sapiens
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid
-
0.0184
N-desmethyltoremifene
Homo sapiens
liver cytosol, pH 7.4, 37°C, substrate lithocholic acid
-
0.0014 - 0.0122
nafoxidine
0.0023 - 0.003
ospemifene
0.00018 - 0.00041
raloxifene
0.0101 - 0.0163
tamoxifen
0.0032 - 0.011
toremifene
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00079
-
using hyodeoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.00163
-
-
0.00248
0.0028
-
using glycochenodeoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.00354
-
using chenodeoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.00359
-
using petromyzonol as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.00492
-
using ursodeoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.00819
-
using deoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.018
-
-
0.02361
-
using lithocholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
additional information
-
continuous fluorimetric assay for alcohol sulfotransferase whoses sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
7.5 - 9
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
5.5 - 10.5
6.5 - 9
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
-
isoelectric focusing electrophoresis
5.4
-
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
high activity fetal, isoform SULT2A1
Manually annotated by BRENDA team
-
fetal, low activity, isoform SULT2A1
Manually annotated by BRENDA team
additional information
-
no activity in kidney
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
no activity in microsomes
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
tamoxifen or raloxifene are linked to the development of cholestasis, and inhibit sulfotransferase 2A1 (SULT2A1)-catalyzed dehydroepiandrosterone (DHEA) sulfonation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ST2A1_HUMAN
285
0
33780
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33780
x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence
34000
x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence
65000
-
gel filtration
67000
-
gel filtration
68000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
repeated freezing and thawing reduces activity considerably, addition of ethylene glycol preserves activity
-
SULT2A1 activity is consistently higher using NaH2PO4 buffer in a pH range from 5.5 to 6.5
SULT2A1 activity is consistently highest using NaH2PO4 buffer in a pH range from 5.5 to 6.5
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30°C, ethylene glycol enzyme solution can be stored for at least 3 months without serious decrease in activity
-
4°C, labile
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in HEK-293 cells
expressed in HEK293 cells
expression in HepG2 cells or CaCo-2 cells
-
gene SULT2A1, recombinant expression
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
assay for bile salt sulfotransferase renders it possible to estimate this enzyme in percutaneous liver biopsy specimens. By application of this technique it will be possible to study this enzyme in patients with various liver diseases
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Loeoef, L.; Wengle, B.
Enzymatic sulphation of bile salts in human liver
Biochim. Biophys. Acta
530
451-460
1978
Homo sapiens
Manually annotated by BRENDA team
Loeoef, L.; Hjerten, S.
Partial purification of a human liver sulphotransferase active towards bile salts
Biochim. Biophys. Acta
617
192-204
1980
Homo sapiens
Manually annotated by BRENDA team
Chen, L.J.
Bile salt sulfotransferase
Methods Enzymol.
77
213-218
1981
Cavia porcellus, Oryctolagus cuniculus, Homo sapiens, Mesocricetus auratus, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Chen, L.J.; Segel, I.H.
Purification and characterization of bile salt sulfotransferase from human liver
Arch. Biochem. Biophys.
241
371-379
1985
Homo sapiens
Manually annotated by BRENDA team
Obinata, K.; Nemeth, A.; Ellin, A.; Strandvik, B.
Bile salt sulphotransferase activity in the liver of cholestatic infants
Scand. J. Clin. Lab. Invest.
54
285-290
1994
Homo sapiens
Manually annotated by BRENDA team
Chen, W.T.; Liu, M.C.; Yang, Y.S.
Fluorometric assay for alcohol sulfotransferase
Anal. Biochem.
339
54-60
2005
Homo sapiens
Manually annotated by BRENDA team
Seely, J.; Amigh, K.S.; Suzuki, T.; Mayhew, B.; Sasano, H.; Giguere, V.; Laganiere, J.; Carr, B.R.; Rainey, W.E.
Transcriptional regulation of dehydroepiandrosterone sulfotransferase (SULT2A1) by estrogen-related receptor alpha
Endocrinology
146
3605-3613
2005
Homo sapiens
Manually annotated by BRENDA team
Stanley, E.L.; Hume, R.; Coughtrie, M.W.H.
Expression profiling of human fetal cytosolic sulfotransferases involved in steroid and thyroid hormone metabolism and in detoxification
Mol. Cell. Endocrinol.
240
32-42
2005
Homo sapiens
Manually annotated by BRENDA team
Echchgadda, I.; Song, C.S.; Roy, A.K.; Chatterjee, B.
Dehydroepiandrosterone sulfotransferase is a target for transcriptional induction by the vitamin D receptor
Mol. Pharmacol.
65
720-729
2004
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Echchgadda, I.; Song, C.S.; Oh, T.; Ahmed, M.; De La Cruz, I.J.; Chatterjee, B.
The xenobiotic-sensing nuclear receptors pregnane X receptor, constitutive androstane receptor, and orphan nuclear receptor hepatocyte nuclear factor 4alpha in the regulation of human steroid-/bile acid-sulfotransferase
Mol. Endocrinol.
21
2099-2111
2007
Homo sapiens (Q06520), Homo sapiens
Manually annotated by BRENDA team
Senggunprai, L.; Yoshinari, K.; Yamazoe, Y.
Inhibitory effects of kynurenic acid, a tryptophan metabolite, and its derivatives on cytosolic sulfotransferases
Biochem. J.
422
455-462
2009
Homo sapiens (Q06520)
Manually annotated by BRENDA team
Kurogi, K.; Krasowski, M.D.; Injeti, E.; Liu, M.Y.; Williams, F.E.; Sakakibara, Y.; Suiko, M.; Liu, M.C.
A comparative study of the sulfation of bile acids and a bile alcohol by the Zebra danio (Danio rerio) and human cytosolic sulfotransferases (SULTs)
J. Steroid Biochem. Mol. Biol.
127
307-314
2011
Homo sapiens
Manually annotated by BRENDA team
Huang, J.; Bathena, S.P.; Tong, J.; Roth, M.; Hagenbuch, B.; Alnouti, Y.
Kinetic analysis of bile acid sulfation by stably expressed human sulfotransferase 2A1 (SULT2A1)
Xenobiotica
40
184-194
2010
Homo sapiens, Homo sapiens (Q06520)
Manually annotated by BRENDA team
Bansal, S.; Lau, A.J.
Inhibition of human sulfotransferase 2A1-catalyzed sulfonation of lithocholic acid, glycolithocholic acid, and taurolithocholic acid by selective estrogen receptor modulators and various analogs and metabolites
J. Pharmacol. Exp. Ther.
369
389-405
2019
Homo sapiens (Q06520), Homo sapiens
Manually annotated by BRENDA team