Information on EC 2.7.1.63 - polyphosphate-glucose phosphotransferase

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The expected taxonomic range for this enzyme is: Actinomycetales

EC NUMBER
COMMENTARY hide
2.7.1.63
-
RECOMMENDED NAME
GeneOntology No.
polyphosphate-glucose phosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(phosphate)n + D-glucose = (phosphate)n-1 + D-glucose 6-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
-
-
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
Glycolysis / Gluconeogenesis
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
polyphosphate:D-glucose 6-phosphotransferase
Requires a neutral salt, e.g. KCl, for maximum activity. Also acts on glucosamine.
CAS REGISTRY NUMBER
COMMENTARY hide
9033-50-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene cg2091 or ppgK
Q6M4B1
UniProt
Manually annotated by BRENDA team
strain NM-1
-
-
Manually annotated by BRENDA team
strain NM-1
-
-
Manually annotated by BRENDA team
Mycobacterium jucho
-
-
-
Manually annotated by BRENDA team
Mycobacterium pellegrino
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Nocardia minima
-
-
-
Manually annotated by BRENDA team
orf Tfu_1811
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(phosphate)120 + D-glucose
(phosphate)119 + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
(phosphate)15 + D-glucose
(phosphate)14 + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
(phosphate)20 + D-glucose
(phosphate)19 + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
(phosphate)25 + D-glucose
(phosphate)24 + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
(phosphate)4 + D-glucose
(phosphate)3 + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
(phosphate)45 + D-glucose
(phosphate)44 + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
(phosphate)5 + D-glucose
(phosphate)4 + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
(phosphate)65 + D-glucose
(phosphate)64 + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
(phosphate)75 + D-glucose
(phosphate)74 + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
(phosphate)n + D-glucose
(phosphate)n-1 + D-glucose 6-phosphate
show the reaction diagram
(phosphate)n + D-mannose
(phosphate)n-1 + D-mannose 6-phosphate
show the reaction diagram
(phosphate)n + glucosamine
(phosphate)n-1 + D-glucosamine 6-phosphate
show the reaction diagram
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
CTP + D-glucose
CDP + D-glucose 6-phosphate
show the reaction diagram
CTP + glucose
CDP + D-glucose 6-phosphate
show the reaction diagram
dATP + glucose
dADP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + D-glucose
GDP + D-glucose 6-phosphate
show the reaction diagram
Q6M4B1
-
-
-
?
GTP + glucose
GDP + D-glucose 6-phosphate
show the reaction diagram
ITP + D-glucose
IDP + D-glucose 6-phosphate
show the reaction diagram
TTP + glucose
TDP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
UTP + D-glucose
UDP + D-glucose 6-phosphate
show the reaction diagram
UTP + glucose
UDP + D-glucose 6-phosphate
show the reaction diagram
XTP + glucose
XDP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(phosphate)n + D-glucose
(phosphate)n-1 + D-glucose 6-phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation, about 30% as efficient as Mg2+
Cu2+
-
divalent cation required, 11% of the activity with Mg2+ for the polyphosphate-dependent activity and no activation of ATP-dependent activity, 5 mM
Fe2+
-
divalent cation required, no activation of the polyphosphate-dependent activity and 15% of the activity with Mg2+ for ATP-dependent acticity, 5 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
at high concentrations competitive substrate inhibition with respect to glucose, ATP-dependent reaction
D-fructose 6-phosphate
-
inhibits reaction with polyphosphate and glucose or ATP and glucose
D-glucose
-
above 150 mM, substrate inhibition
D-glucose 6-phosphate
D-xylose
Mg2+
-
molar ratio polyphosphate/Mg below 0.5, activation above, EDTA partially reverses
NaCl
Nocardia minima
-
above 100 mM
Poly(P)
-
above 0.05 mM, substrate inhibition
Tetrapolyphosphate
-
inhibits reaction with ATP
tripolyphosphate
-
when present in excess together with Grahams salt at pH 7.2
additional information
Q6M4B1
no effect by fructose 1,6-bisphosphate, xylose, cAMP, UDP glucose, and glucose 1,6-bisphosphate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
Q6M4B1
no effect by fructose 1,6-bisphosphate, xylose, cAMP, UDP glucose, and glucose 1,6-bisphosphate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
(phosphate)120
Q6M4B1
pH 7.0, temperature not specified in the publication
-
0.6
(phosphate)15
Q6M4B1
pH 7.0, temperature not specified in the publication
0.4
(phosphate)20
Q6M4B1
pH 7.0, temperature not specified in the publication
0.3
(phosphate)25
Q6M4B1
pH 7.0, temperature not specified in the publication
-
0.5
(phosphate)4
Q6M4B1
pH 7.0, temperature not specified in the publication
0.2
(phosphate)45
Q6M4B1
pH 7.0, temperature not specified in the publication
-
0.8
(phosphate)5
Q6M4B1
pH 7.0, temperature not specified in the publication
0.1
(phosphate)65
Q6M4B1
pH 7.0, temperature not specified in the publication
-
0.3
(phosphate)75
Q6M4B1
pH 7.0, temperature not specified in the publication
-
0.15 - 6
ATP
3.9 - 5.9
CTP
0.06 - 1.1
D-glucose
0.5
glucose
-
pH 7.0, 30C
0.8 - 3.5
GTP
0.02
Hexametaphosphate
-
pH 7.0, 30C
0.8
ITP
Q6M4B1
pH 7.0, temperature not specified in the publication
0.15
mannose
-
pH 7.0, 30C
0.175
Poly(P)
-
pH 8.5, 37C, in terms of acid-labile phosphate
0.033 - 0.082
poly(P10)
0.0072 - 0.13
poly(P15)
3.8
poly(P30)
-
-
-
0.0086
poly(P347)
-
pH 7.5, 30C
-
0.0012 - 0.0088
poly(P35)
0.000005 - 0.037
poly(P400)
0.0067
poly(P430)
-
pH 7.5, 30C
-
0.06
poly(P700)
-
-
-
0.012
poly(P8)
-
-
-
1.5 - 2.6
UTP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.3
(phosphate)120
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
-
6
(phosphate)15
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
8.5
(phosphate)20
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
10
(phosphate)25
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
-
2.5
(phosphate)4
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
8.3
(phosphate)45
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
-
0.2
(phosphate)5
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
11.6
(phosphate)65
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
-
15.4
(phosphate)75
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
-
0.4 - 116
ATP
0.1 - 7.4
CTP
33.6 - 136
D-glucose
14
dATP
Propionibacterium freudenreichii subsp. shermanii
-
pH 7.5, 30C
108 - 116
glucose
1.3 - 11
GTP
3.3
ITP
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
270
poly(P30)
Microlunatus phosphovorus
-
-
-
56
poly(P31)
Propionibacterium freudenreichii subsp. shermanii
-
pH 7.5, 30C
-
55 - 196
poly(P35)
41 - 371
poly(P400)
183
poly(P700)
Microlunatus phosphovorus
-
-
-
87
poly(P8)
Microlunatus phosphovorus
-
-
-
0.3 - 7
UTP
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
113
(phosphate)120
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
42452
10
(phosphate)15
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
14721
21.3
(phosphate)20
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
12238
33.3
(phosphate)25
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
8808
5
(phosphate)4
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
8814
41.5
(phosphate)45
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
5045
0.3
(phosphate)5
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
42450
116
(phosphate)65
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
4176
51.3
(phosphate)75
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
42451
0.07
ATP
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
4
0.03
CTP
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
60
64.4 - 151
D-glucose
0.4
GTP
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
37
4.1
ITP
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
229
0.2
UTP
Corynebacterium glutamicum
Q6M4B1
pH 7.0, temperature not specified in the publication
65
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11
ADP
Q6M4B1
pH 7.0, temperature not specified in the publication
15
AMP
Q6M4B1
pH 7.0, temperature not specified in the publication
6.6 - 7.4
ATP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64.6
-
purified recombinant free cellulose-binding module fusion enzyme, pH 7.5, 50C
110
-
reaction with ATP and glucose
203
polyphosphate-dependent activity
220
-
reaction with polyphosphate and glucose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 8.3
-
broad plateau
7.5
-
polyphosphate- and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 11
-
activity range, profile, overview. 40% of maximal activity at pH 7.5, 50C, 31% at pH 9.0, 50C, recombinant enzyme fused with a family 3 cellulose-binding module
5.7 - 8.4
Q6M4B1
84% activity at pH 6.4 in 50 mM MES and 73% activity at pH 8.4 in 50 mM glycylglycine
6 - 9.5
-
pH 6.0: about 25% of maximal activity, pH 9.5: about 35% of maximal activity, polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity
6 - 8.4
-
very little activity below pH 6.0 and above pH 8.4
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 80
-
activity range, profile, overview. 40% of maximal activity at 50C, maximal activity at 55C, at pH 7.5, recombinant enzyme fused with a family 3 cellulose-binding module
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
Arthrobacter sp. (strain KM)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000
Nocardia minima
-
gel filtration
64000
-
gel filtration
66000
-
gel filtration
67800
Q6M4B1
recombinant detagged enzyme, gel filtration
113000
-
sucrose density gradient centrifugation
275000 - 280000
-
non-denaturing PAGE, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme, hanging drop vapour diffusion method, cyrstallization drop on a silconized coverslip by mixing of 0.003 ml protein solution consisting of 10 mg/ml protein in 10 mM glucose, and 10 mM potassium phosphate, pH 7.0, with an equal volume of mother liquor composed of 2.0 M ammonium sulfate, 2% v/v PEG 400, and 0.1 M HEPES, pH 8.0, cyrstals are soaked in several heavy atom derivative solutions containing 1 mM of UO2Ac2, 10 mM AgNO3, 10 mM GdCl2, 1 mM HgCl2, 10 mM K2Pt(CN)4, 2 mM TmCl2, or 1 mM EuCl3 in 0.1 M Tris-HCl at different pH-values, dependent on the heavy atom salt, for 20-60 min at 20C, X-ray diffraction structure determiination and analysis at 3.0 A resolution, modeling
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
-
t1/2: 24 h at 3C or at 37C plus 0.3 M KCl
641743
5 - 7
-
24 h stable at 3C
641743
8.1 - 8.4
-
immobilized enzyme is stable
641744
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 40
Q6M4B1
purified recombinant enzyme, 100% remaining activity after 1 h
37
-
24 h, pH 4-5: precipitation and 80% of activity retained, above pH 5.5: inactivation within 24 h, 0.3 M KCl stabilizes
40
-
5 min, 50% loss of activity
50
Q6M4B1
purified recombinant enzyme, 12% remaining activity after 1 h
60
Q6M4B1
purified recombinant enzyme, no remaining activity after 5 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysed enzyme preparations are largely inactive, Mg2+ restores activity
-
dialysis against distilled water for 10 h leads to decreased activity
-
enzyme immobilized on corn stover is stable for one months at pH 8.5, 30C
-
KCl, 0.3 M, stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
frozen, partially purified preparation, several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
not separable from ATP-glucokinase
-
not separable from ATP-glucokinase; partial
-
partial
recombinant enzyme from Escherichia coli in several steps
-
recombinant enzyme fused with a family 3 cellulose-binding module 3.2fold from Escherichia coli strain BL21(DE3) in a one-step purification and immobilization on regenerated amorphous cellulose
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and His-tag cleavage with factor Xa
Q6M4B1
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
gene cg2091 or ppgK, sequence comparisons and phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Q6M4B1
gene GMK, expression in Escherichia coli strain BL21(DE3)
-
orf Tfu_1811 overexpressed as protein fused with a family 3 cellulose-binding module in Escherichia coli strain BL21 (DE3)
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
immobilization of the recombinant cellulose-binding module fusion enzyme, which shows about 7.7fold increased catalytic turnover compared to the free enzyme
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