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Literature summary for 2.7.1.63 extracted from
- Initial rate and equilibrium isotope exchange studies on the ATP-dependent activity of polyphosphate Glucokinase from Propionibacterium shermanii (1996), Biochemistry, 35, 6777-6785.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibits reaction with ATP | Propionibacterium freudenreichii subsp. shermanii |  |
| D-glucose 6-phosphate | inhibits reaction with ATP | Propionibacterium freudenreichii subsp. shermanii | |
| D-xylose | inhibits reaction with ATP | Propionibacterium freudenreichii subsp. shermanii | |
| Tetrapolyphosphate | inhibits reaction with ATP | Propionibacterium freudenreichii subsp. shermanii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
(phosphate)31 | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 0.8 | - |
GTP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 1.2 | - |
dATP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 1.5 | - |
ATP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 2.6 | - |
UTP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 5.9 | - |
CTP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| not separable from ATP-glucokinase | Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (phosphate)31 + D-glucose | - |
Propionibacterium freudenreichii subsp. shermanii | (phosphate)30 + D-glucose 6-phosphate | - |
? | |
| (phosphate)n + D-glucose | the enzyme utilizes polyphosphate much more efficiently than it does ATP, with a turnover number/Kpolyphosphate to turnover number/KATP ratio of 2800 | Propionibacterium freudenreichii subsp. shermanii | (phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | the enzyme utilizes polyphosphate much more efficiently than it does ATP, with a turnover number/Kpolyphosphate to turnover number/KATP ratio of 2800 | Propionibacterium freudenreichii subsp. shermanii | ADP + D-glucose 6-phosphate | - |
? | |
| CTP + glucose | - |
Propionibacterium freudenreichii subsp. shermanii | CDP + D-glucose 6-phosphate | - |
? | |
| dATP + glucose | - |
Propionibacterium freudenreichii subsp. shermanii | dADP + D-glucose 6-phosphate | - |
? | |
| GTP + glucose | - |
Propionibacterium freudenreichii subsp. shermanii | GDP + D-glucose 6-phosphate | - |
? | |
| UTP + glucose | - |
Propionibacterium freudenreichii subsp. shermanii | UDP + D-glucose 6-phosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7 | - |
UTP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 7.4 | - |
CTP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 11 | - |
GTP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 14 | - |
dATP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 25 | - |
ATP | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii | |
| 56 | - |
(phosphate)31 | pH 7.5, 30°C | Propionibacterium freudenreichii subsp. shermanii |
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