Information on EC 2.1.1.42 - flavone 3'-O-methyltransferase

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The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY
2.1.1.42
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RECOMMENDED NAME
GeneOntology No.
flavone 3'-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
S-adenosyl-L-methionine + 3'-hydroxyflavone = S-adenosyl-L-homocysteine + 3'-methoxyflavone
show the reaction diagram
also acts on luteolin 7-O-beta-D-glucoside
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
methyl group transfer
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PATHWAY
KEGG Link
MetaCyc Link
chrysoeriol biosynthesis
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methylquercetin biosynthesis
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polymethylated quercetin biosynthesis
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quercetin sulfate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:3'-hydroxyflavone 3'-O-methyltransferase
The enzyme prefers flavones with vicinal 3',4'-dihydroxyl groups.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3'-O-methyltransferase
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caffeic acid/5-hydroxyferulic acid O-methyltransferase
Q9FK25
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HvOMT1
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gene name
luteolin 3'-O-methyltransferase
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luteolin methyltransferase
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o-dihydric phenol meta-O-methyltransferase
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o-dihydric phenol methyltransferase
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o-diphenol m-O-methyltransferase
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O-methyltransferase
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OMT-15
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OMT-17
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S-adenosylmethionine:flavone/flavonol 3'-O-methyltransferase
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SOMT-9
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ZmOMT1
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gene name
CAS REGISTRY NUMBER
COMMENTARY
37205-55-3
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
soybean, L. var. Mandarin
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Manually annotated by BRENDA team
tobacco, Samsun N N.
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Manually annotated by BRENDA team
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
S-adenosyl-L-methionine
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ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
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extractable activity drastically increases by illuminating cells with white light
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SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.000027
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-
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0.0029
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pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
10
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TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
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assay at
37
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assay at
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.48
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calculated from sequence
5.64
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calculated from sequence
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
9.8
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at higher pH values activity rapidly declines
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, stored in 10% glycerol for 2 months, no singnificant loss in enzyme activity
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4C, highly unstable, stored with Triton X-100, bovine serum albumin, ethylene glycol, dimethyl sulfoxide, 2-mercaptoethanol, dithiothreitol or ethylene glycol monoethylether, loses over 90% of its activity within 94 h
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4C, stable when stored in buffer containing 10% (v/v) glycerol for 34 weeks
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-20C, 1-3 mg protein/ml in 0.02 M Tris-HCl buffer loses about 30% of its activity after 1 month
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4C, acetone powder of fresh cells as source of the enzyme can be kept without significant loss of enzyme activity
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4C, stable when stored in buffer containing 10% (v/v) glycerol for 3-4 weeks
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