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Literature summary for 2.1.1.42 extracted from

  • Zhou, J.-M.; Fukushi, Y.; Wollenweber, E.; Ibrahim, R.K.
    Characterization of two O-methyltransferase-like genes in barley and maize (2008), Pharm. Biol., 46, 26-34.
No PubMed abstract available
Search for more literature for 2.1.1.42

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hordeum vulgare
expression in Escherichia coli Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00618
-
 5,7,3',4'-tetrahydroxyflavone 30°C Hordeum vulgare
0.0159
-
 5,7,3',4'-tetrahydroxyflavone 30°C Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
additional information requires no Mg2+ Hordeum vulgare
additional information requires no Mg2+ Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38700
-
 x * 38700, calculated from sequence Hordeum vulgare
39600
-
 x * 39600, calculated from sequence Zea mays

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hordeum vulgare
-
 Zea mays

Storage Stability

Storage Stability Organism
4°C, stable when stored in buffer containing 10% (v/v) glycerol for 3-4 weeks Zea mays
4°C, stable when stored in buffer containing 10% (v/v) glycerol for 3–4 weeks Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone i.e. luteolin, the enzyme also catalyzes the methylation of tricetin and the methylation of quercetin, prefers tricetin over luteolin Hordeum vulgare S-adenosyl-L-homocysteine + 3'-O-methylluteolin
-
 ?
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone i.e. lutepolin, the enzyme also catalyzes the methylation of tricetin and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin Zea mays S-adenosyl-L-homocysteine + 3'-O-methylluteolin
-
 ?

Subunits

Subunits Comment Organism
? x * 38700, calculated from sequence Hordeum vulgare
? x * 39600, calculated from sequence Zea mays

Synonyms

Synonyms Comment Organism
HvOMT1
-
 Hordeum vulgare
ZmOMT1
-
 Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Hordeum vulgare
30
-
 assay at Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Hordeum vulgare
7.5
-
-
 Zea mays

pI Value

Organism Comment pI Value Maximum pI Value
Zea mays calculated from sequence
-
 5.48
Hordeum vulgare calculated from sequence
-
 5.64