Information on EC 1.20.4.2 - methylarsonate reductase

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The expected taxonomic range for this enzyme is: Euarchontoglires

EC NUMBER
COMMENTARY
1.20.4.2
-
RECOMMENDED NAME
GeneOntology No.
methylarsonate reductase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
arsenate detoxification I (glutaredoxin)
-
SYSTEMATIC NAME
IUBMB Comments
gluthathione:methylarsonate oxidoreductase
The product, Me-As(OH)2 (methylarsonous acid), is biologically methylated by EC 2.1.1.137, arsenite methyltransferase, to form cacodylic acid (dimethylarsinic acid).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
GSTO1
-
-
GSTO2
-
-
MMA(V) reductase
-
-
-
-
reductase, methylarsonate
-
-
-
-
monomethylarsonic acid (MMA V) reductase/hGSTO1
-
-
additional information
-
identical to omega class glutathione-S-transferase (hGSTO1-1)
CAS REGISTRY NUMBER
COMMENTARY
254889-62-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
isoforms GSTO1, GSTO2 and their natural polymorphisms. Enzyme has additionally thioltransferase, dehydroascorbate reductase and dimethylarsonate reductase activities
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
arsenate + glutathione
arsenite + reduced glutathione
show the reaction diagram
-
-
-
-
?
dimethylarsonate + glutathione
dimethylarsonous acid
show the reaction diagram
-
-
-
-
?
methylarsonate + glutathione
methylarsonite + oxidized glutathione
show the reaction diagram
-
rate-limiting enzyme in biotransformation of inorganic arsenite in rabbit liver
-
-
?
methylarsonate + glutathione
methylarsonite + glutathione disulfide
show the reaction diagram
P78417
-
-
-
?
methylarsonate + glutathione
methylarsonite + glutathione disulfide
show the reaction diagram
-
-, involved in biotransformation of arsenate
-
-
?
methylarsonate + glutathione
methylarsonite + glutathione disulfide
show the reaction diagram
P78417
rate limiting enzyme of inorganic arsenic metabolism
-
-
?
monomethylarsonate + glutathione
monomethylarsonous acid + oxidized glutathione
show the reaction diagram
-
-
-
-
?
monomethylarsonate + glutathione
monomethylarsonous acid + oxidized glutathione
show the reaction diagram
P78417
-
-
-
?
monomethylarsonate + glutathione
monomethylarsonous acid + oxidized glutathione
show the reaction diagram
-
-
-
?
monomethylarsonate + glutathione
monomethylarsonous acid + oxidized glutathione
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
methylarsonate + glutathione
methylarsonite + oxidized glutathione
show the reaction diagram
-
rate-limiting enzyme in biotransformation of inorganic arsenite in rabbit liver
-
-
?
methylarsonate + glutathione
methylarsonite + glutathione disulfide
show the reaction diagram
-
involved in biotransformation of arsenate
-
-
?
methylarsonate + glutathione
methylarsonite + glutathione disulfide
show the reaction diagram
P78417
rate limiting enzyme of inorganic arsenic metabolism
-
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Sodium deoxycholate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
dithiothreitol
-
stimulates
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
20.9
-
dimethylarsonate
-
-
2.1
-
monomethylarsonate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.27
-
P78417
GSTO1 mutant A140D, 30C, pH 5.5
0.33
-
P78417
wild-type GSTO1, 30C, pH 5.5
0.39
-
P78417
GSTO1 mutant E208K, 30C, pH 5.5
0.42
-
P78417
wild-type GSTO1, 30C, pH 5.5
0.44
-
P78417
GSTO2 mutant N142D, 30C, pH 5.5
0.65
-
P78417
GSTO1 mutant DELTAE155, 30C, pH 5.5
0.67
-
P78417
GSTO1 mutant DELTAE155/E208K, 30C, pH 5.5
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8
8.2
-
-
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28000
-
-
SDS-PAGE
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
P78417
wild-type, 30 min, 60% residual activity. GSTO1 mutant E208K, 30 min, 80% residual activity, GSTO1 mutant DELTAE155/E208K, 30 min, no residual activity
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
A140D
P78417
isoform GSTO2, natural polymorphism, no significant alteration in monomethylarsonate reductase activity
DELTAE155
P78417
isoform GSTO2, natural polymorphism, 90% increase in monomethylarsonate reductase activity
DELTAE155/E208K
P78417
isoform GSTO2, natural polymorphism, 100% increase in monomethylarsonate reductase activity, decrease in heat stability
N142D
P78417
isoform GSTO2, natural polymorphism, no significant alteration in monomethylarsonate reductase activity
E208K
P78417
isoform GSTO2, natural polymorphism, slight increase in monomethylarsonate reductase activity, decrease in heat stability
additional information
-
naturally occurring mutations S86C, A140D, E155del, A236V, and E208K significantly decrease enzyme activity