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0.00007 - 1.54
5-phospho-alpha-D-ribose 1-diphosphate
0.000005 - 0.297
anthranilate
0.00007
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 2 mM Mg2+, mutant enzyme D223N
0.00016
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 0.05 mM Mg2+, wild-type enzyme
0.00017
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A
0.00026
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q
0.0003
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A
0.002
5-phospho-alpha-D-ribose 1-diphosphate
25°C
0.0021
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.0024
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.0026
5-phospho-alpha-D-ribose 1-diphosphate
40°C
0.0026
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 2 mM Mg2+, mutant enzyme E224Q
0.0032
5-phospho-alpha-D-ribose 1-diphosphate
50°C
0.0035
5-phospho-alpha-D-ribose 1-diphosphate
60°C
0.0043
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme
0.005
5-phospho-alpha-D-ribose 1-diphosphate
87°C
0.01
5-phospho-alpha-D-ribose 1-diphosphate
-
0.013
5-phospho-alpha-D-ribose 1-diphosphate
-
TAX6trpR782
0.013
5-phospho-alpha-D-ribose 1-diphosphate
mutant D83G/F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.019
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.02
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.0224
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.023
5-phospho-alpha-D-ribose 1-diphosphate
mutant D83G/F149S, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.032
5-phospho-alpha-D-ribose 1-diphosphate
at pH 7.5 and 25°C
0.033
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C
0.037
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C
0.038
5-phospho-alpha-D-ribose 1-diphosphate
mutant D83G, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.045
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C
0.047
5-phospho-alpha-D-ribose 1-diphosphate
wild-type, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.047
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C
0.054
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C
0.055
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C
0.055
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C
0.06
5-phospho-alpha-D-ribose 1-diphosphate
-
trpAB1653trpR782
0.062
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C
0.085
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.086
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.1
5-phospho-alpha-D-ribose 1-diphosphate
-
complex
0.116
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.15
5-phospho-alpha-D-ribose 1-diphosphate
I36E/M47D double mutant, pH 7.2, 37°C, 0.01 microM subunit concentration
0.15
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.151
5-phospho-alpha-D-ribose 1-diphosphate
mutant F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.18
5-phospho-alpha-D-ribose 1-diphosphate
wild-type, pH 7.2, 37°C, 0.01 microM subunit concentration
0.2
5-phospho-alpha-D-ribose 1-diphosphate
-
component II
0.25
5-phospho-alpha-D-ribose 1-diphosphate
at pH 8.5 and 55°C
0.371
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.88
5-phospho-alpha-D-ribose 1-diphosphate
-
-
1.54
5-phospho-alpha-D-ribose 1-diphosphate
wild-type, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.000005
anthranilate
at 87°C, Km decreases at lower temperatures
0.000018
anthranilate
wild-type, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.00002
anthranilate
wild-type, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.00004
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+, wild-type enzyme
0.000058
anthranilate
I36E/M47D double mutant, pH 7.2, 37°C, 0.01 microM subunit concentration
0.00007
anthranilate
60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme
0.000085
anthranilate
wild-type, pH 7.2, 37°C, 0.01 microM subunit concentration
0.00012
anthranilate
mutant D83G, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.00016
anthranilate
60°C, pH 7.5, 2 mM Mg2+,mutant enzyme D223N
0.00053
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A
0.00075
anthranilate
60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q
0.00091
anthranilate
60°C, pH 7.5, 2 mM Mg2+,mutant enzyme E224Q
0.0012
anthranilate
mutant F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.0022
anthranilate
at pH 8.5 and 55°C
0.003
anthranilate
-
TAX6trpR782
0.0031
anthranilate
mutant D83G/F149S, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.0036
anthranilate
mutant D83G/F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.004
anthranilate
-
trpAB1653trpR782
0.007
anthranilate
mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C
0.008
anthranilate
mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C
0.011
anthranilate
mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C
0.0125
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A/P178A
0.029
anthranilate
mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C
0.037
anthranilate
mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C
0.051
anthranilate
mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C
0.06
anthranilate
mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C
0.082
anthranilate
mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C
0.149
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+,mutant enzyme R164A/H154A
0.297
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A