2.4.1.231: alpha,alpha-trehalose phosphorylase (configuration-retaining)
This is an abbreviated version!
For detailed information about alpha,alpha-trehalose phosphorylase (configuration-retaining), go to the full flat file.
Reaction
Synonyms
PoTPase, ScTPase, TP2, TP3, trehalose phosphorylase
ECTree
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Engineering
Engineering on EC 2.4.1.231 - alpha,alpha-trehalose phosphorylase (configuration-retaining)
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D379N
ratio of phosphorolysis to hydrolysis decreases by a factor of 287500
H403A
ratio of phosphorolysis to hydrolysis decreases by a factor of 2190
K512A
R507A
D379N
-
ratio of phosphorolysis to hydrolysis decreases by a factor of 287500
-
H403A
-
ratio of phosphorolysis to hydrolysis decreases by a factor of 2190
-
K512A
-
ratio of phosphorolysis to hydrolysis decreases by a factor of 511111
-
R507A
-
ratio of phosphorolysis to hydrolysis decreases by a factor of 575000
-
ratio of phosphorolysis to hydrolysis decreases by a factor of 511111
K512A
-
kcat/Km (phosphate): 0.0014/Msec, vanadate shows a weaker inhibitory effect compared to wild-type, propargylamine leads to a 23fold increase in activity, phosphate 50 mM enhances basal hydrolase activity
ratio of phosphorolysis to hydrolysis decreases by a factor of 575000
R507A
-
Km (phosphate) 103 mM, kcat/Km (phosphate): 0.0004/Msec, vanadate shows no inhibitory effect, guanidine leads to a 45fold increase in activity