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2.3.1.B27: protein acetyltransferase (Alba1-protein acetylating)

This is an abbreviated version!
For detailed information about protein acetyltransferase (Alba1-protein acetylating), go to the full flat file.

Reaction

acetyl-CoA
+
[DNA-binding protein Alba1]-L-lysine16
=
CoA
 +
[DNA-binding protein Alba1]-N6-acetyl-L-lysine16

Synonyms

SSO2813, ssPat

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.B27 protein acetyltransferase (Alba1-protein acetylating)

Engineering

Engineering on EC 2.3.1.B27 - protein acetyltransferase (Alba1-protein acetylating)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D29A
reduction in activity by about 2fold
D53N
modest reduction in activity
E42Q
modest reduction in activity
E43Q
modest reduction in activity
E76A
activity is reduced to near background levels
E76Q
mutation has no effect on activity
H36A
mutant shows activity similar to the wild type enzyme
H72A/E76Q
activity is reduced to near background levels
M121H
mutation results in about a 2fold increase in protein substrate Km and about a 5fold decrease in overall kcat, despite little change in the Km for acetyl-CoA
M121Y
mutation results in about a 2fold increase in protein substrate Km and about a 5fold decrease in overall kcat, despite little change in the Km for acetyl-CoA
R33A
reduction in activity to near background levels. Km for Ac-CoA is similar to wild type and elevated by about 2fold for protein substrate, whereas the overall kcat is reduced about 5fold relative to the wild type protein
S78A
the activity for both mutants is close to wild type
S78C
the activity for both mutants is close to wild type
Y38S
modest reduction in activity
D29A
-
reduction in activity by about 2fold
-
E42Q
-
modest reduction in activity
-
E76Q
-
mutation has no effect on activity
-
H36A
-
mutant shows activity similar to the wild type enzyme
-
R33A
-
reduction in activity to near background levels. Km for Ac-CoA is similar to wild type and elevated by about 2fold for protein substrate, whereas the overall kcat is reduced about 5fold relative to the wild type protein
-