Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.1.1.171: 16S rRNA (guanine966-N2)-methyltransferase

This is an abbreviated version!
For detailed information about 16S rRNA (guanine966-N2)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.171

Reaction

S-adenosyl-L-methionine
+
guanine966 in 16S rRNA
=
S-adenosyl-L-homocysteine
 +
N2-methylguanine966 in 16S rRNA

Synonyms

16S rRNA-specific methyltransferases m2GMT, EC 2.1.1.51, EC 2.1.1.52, m2G966 methyltransferase, RNA:(guanine-N2) methyltransferase RsmD, RsmD, RsmD-like methyltransferase, Rv2966c, Rv2966c protein, small rRNA methyltransferase, yhhF

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.171 16S rRNA (guanine966-N2)-methyltransferase

Engineering

Engineering on EC 2.1.1.171 - 16S rRNA (guanine966-N2)-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D58A
-
site-directed mutagenesis, the mutant shows increased Km toward the 30S subunit by two orders of magnitude with only a marginal effect on kcat compared to the wild-type enzyme. The mutation decreases the enthalpic contribution to SAM binding, paralleled by an increase in the entropic contribution
D58A/P128A/P129A
-
site-directed mutagenesis, the mutant shows increased Km toward the 30S subunit by two orders of magnitude with only a marginal effect on kcat compared to the wild-type enzyme
F130S
-
site-directed mutagenesis
P128A/P129A
-
site-directed mutagenesis, the mutation decreases the enthalpic contribution to SAM binding, paralleled by an increase in the entropic contribution,
P128A/P129A/F130S
-
site-directed mutagenesis, inactive mutant
additional information
-
knockout of the rsmD gene and complementation by point mutants, except for mutant P128A/P129A/F130S, overview