1.2.3.4: oxalate oxidase

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For detailed information about oxalate oxidase, go to the full flat file.

Reaction

Synonyms

aero-oxalo dehydrogenase, Germin, Germin GF-2.8, Germin GF-3.8, germin-like oxidase, germin-like protein, gl-OXO, glp1, GLP2, HvOxo1, oxalate oxidase, oxalate oxidase GF-2.8, oxalate: O2 oxidoreductase, oxalate:oxygen oxidoreductase, oxalic acid oxidase, OXO, OXO-G, OXO1, OXO2, OXO3, OXO4, OxOx, TaOxo1, TaOxo2

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.3 With oxygen as acceptor

                1.2.3.4 oxalate oxidase

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Results	in table
60	Activating Compound
67	AA Sequence
16	Application
1	CAS Registry Number
21	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
67	Disease/ Diagnostics
2	Expression
8	General Information
9	General Stability
4	IC50 Value
123	Inhibitors
1	kcat/KM [mM/s]
7	Ki Value [mM]
48	KM Value [mM]
19	Localization
44	Metals/Ions
37	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
97	Organism
2	Oxidation Stability
2	Pathway
9	PDB ID
41	pH Optimum
9	pH Range
2	pH Stability
3	pI Value
8	Posttranslational Modification
10	Protein Variants
25	Purification (Commentary)
2	Reaction
11	Reaction Type
89	Reference
58	Source Tissue
45	Specific Activity [micromol/min/mg]
10	Storage Stability
80	Substrates and Products (Substrate)
27	Subunits
69	Synonyms
1	Systematic Name
24	Temperature Optimum [°C]
4	Temperature Range [°C]
15	Temperature Stability [°C]
3	Turnover Number [1/s]

Engineering

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Engineering on EC 1.2.3.4 - oxalate oxidase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E216Q

Bacillus subtilis

-

mutant exhibits a 200fold loss in decarboxylase activity and a 10fold increase in oxalate oxidase activity

678229

A242E

Gelatoporia subvermispora

-

the mutant shows 5% of the wild type oxidase activity

726327

D241A

Gelatoporia subvermispora

-

the mutant contains 17% of the Mn2+ content of the recombinant wild type enzyme and 7% of the wild type oxidase activity

726327

D241S

Gelatoporia subvermispora

-

inactive

726327

R169K

Gelatoporia subvermispora

-

inactive

726327

R349K

Gelatoporia subvermispora

-

inactive

726327

N75A

Hordeum vulgare

P45850

reduced activity to 2.4% of the wild type level

674758

N85A

Hordeum vulgare

P45850

reduced activity to 0.5% of the wild type level

674758

S49A

2 entries

S49A

Hordeum vulgare

-

nonglycosylated oxalate oxidase is produced by site-directed mutagenesis (S49A)

674894

S49A

Triticum aestivum

P15290

the mutant retains essentially full enzyme activity but exhibits altered protein-protein interactions

684846