1.14.99.15: 4-methoxybenzoate monooxygenase (O-demethylating)
This is an abbreviated version!
For detailed information about 4-methoxybenzoate monooxygenase (O-demethylating), go to the full flat file.
Word Map on EC 1.14.99.15
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1.14.99.15
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putida
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rhodopseudomonas
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palustris
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heme
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benzoic
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ferredoxins
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para-substituted
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regioselectivity
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o-demethylation
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2-naphthoic
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benzene
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putidaredoxin
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dioxygen
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4-hydroxybenzoate
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substrate-free
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couplers
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synthesis
- 1.14.99.15
- putida
- rhodopseudomonas
- palustris
- heme
-
benzoic
- ferredoxins
-
para-substituted
-
regioselectivity
-
o-demethylation
-
2-naphthoic
- benzene
- putidaredoxin
- dioxygen
- 4-hydroxybenzoate
-
substrate-free
-
couplers
- synthesis
Reaction
Synonyms
4-methoxybenzoate 4-monooxygenase (O-demethylating), 4-methoxybenzoate monooxygenase, 4-methoxybenzoate O-demethylase, CYP199A2, CYP199A4, cytochrome P450 199A2, oxygenase, 4-methoxybenzoate 4-mono- (O-demethylating), p-anisic O-demethylase, piperonylate-4-O-demethylase, RPA1871
ECTree
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Purification
Purification on EC 1.14.99.15 - 4-methoxybenzoate monooxygenase (O-demethylating)
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recombinant enzyme from Escherichia coli strain BL21(DE3) by two different steps of anion exchange chromatography, followed by gel filtration
recombinant His-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography and gel filtration
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recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by two different steps of anion exchange chromatography, followed by gel filtration