1.1.3.16: ecdysone oxidase
This is an abbreviated version!
For detailed information about ecdysone oxidase, go to the full flat file.
Word Map on EC 1.1.3.16
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1.1.3.16
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ecdysteroids
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3-dehydroecdysone
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midgut
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molting
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silkworm
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instar
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larval
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20-hydroxyecdysone
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spodoptera
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metamorphosis
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lepidoptera
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littoralis
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ecdysterone
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hemolymph
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prothoracic
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manduca
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3beta-hydroxysteroid
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sexta
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leafworm
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beta-reductase
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pupae
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fifth-instar
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glucose-methanol-choline
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blowfly
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malpighian
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alpha-hydroxy
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cis-regulatory
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oxygen-dependent
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hornworm
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hemocytes
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larval-pupal
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calliphora
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silk
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nadp-binding
- 1.1.3.16
- ecdysteroids
- 3-dehydroecdysone
- midgut
-
molting
- silkworm
-
instar
- larval
- 20-hydroxyecdysone
-
spodoptera
-
metamorphosis
- lepidoptera
- littoralis
- ecdysterone
- hemolymph
-
prothoracic
- manduca
-
3beta-hydroxysteroid
- sexta
- leafworm
-
beta-reductase
- pupae
-
fifth-instar
-
glucose-methanol-choline
- blowfly
-
malpighian
-
alpha-hydroxy
-
cis-regulatory
-
oxygen-dependent
- hornworm
-
hemocytes
-
larval-pupal
- calliphora
-
silk
-
nadp-binding
Reaction
Synonyms
beta-ecdysone oxidase, DmEO, ecdysone oxidase, EO, EO2, oxidase, ecdysone
ECTree
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Substrates Products
Substrates Products on EC 1.1.3.16 - ecdysone oxidase
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REACTION DIAGRAM
5,20-dihydroxyecdysone + O2
3-dehydro-5,20-dihydroxyecdysone + H2O2
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-
-
-
?
ecdysone + oxidized 2,6-dichloroindophenol
3-dehydroecdysone + reduced 2,6-dichloroindophenol
3-dehydro-2-deoxy-20-hydroxyecdysone + H2O2
-
-
-
-
?
2-deoxy-20-hydroxyecdysone + O2
3-dehydro-2-deoxy-20-hydroxyecdysone + H2O2
-
-
-
?
20-hydroxyecdysone + O2
3-dehydro-20-hydroxyecdysone + H2O2
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probably part of the catabolic system that inactivates the molting hormones
-
-
?
20-hydroxyecdysone + O2
3-dehydro-20-hydroxyecdysone + H2O2
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first step in reaction sequence for the conversion of ecdysone and 20-hydroxyecdysone to their 3(alpha)-epimers
-
-
?
3-dehydroecdysone + H2O2
-
i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
ecdysone + O2
3-dehydroecdysone + H2O2
-
artificial electron acceptors in vitro in ascending order: ferricyanide, methylene blue, 2,6-dichloroindophenol, 5-methazine methyl sulfate
-
-
?
ecdysone + O2
3-dehydroecdysone + H2O2
-
artificial electron acceptors in vitro in ascending order: ferricyanide, methylene blue, 2,6-dichloroindophenol, 5-methazine methyl sulfate
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
ecdysone + O2
3-dehydroecdysone + H2O2
-
2,6-dichloroindophenol can act as acceptor
-
-
?
ecdysone + O2
3-dehydroecdysone + H2O2
-
2,6-dichloroindophenol can act as acceptor
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
ecdysone + O2
3-dehydroecdysone + H2O2
-
FAD, NAD+, NADP+, and FMN can serve as hydrogen acceptors
-
-
?
ecdysone + O2
3-dehydroecdysone + H2O2
-
i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
ecdysone + O2
3-dehydroecdysone + H2O2
-
probably part of the catabolic system that inactivates the molting hormones
-
-
?
ecdysone + O2
3-dehydroecdysone + H2O2
-
i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
ecdysone + O2
3-dehydroecdysone + H2O2
-
i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
ecdysone + O2
3-dehydroecdysone + H2O2
-
i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
ecdysone + O2
3-dehydroecdysone + H2O2
-
i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
ecdysone + O2
3-dehydroecdysone + H2O2
-
i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
ecdysone + O2
3-dehydroecdysone + H2O2
-
first step in reaction sequence for the conversion of ecdysone and 20-hydroxyecdysone to their 3(alpha)-epimers
-
-
?
ecdysone + O2
3-dehydroecdysone + H2O2
-
i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one
i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione
ir
3-dehydroecdysone + reduced 2,6-dichloroindophenol
-
-
-
-
r
ecdysone + oxidized 2,6-dichloroindophenol
3-dehydroecdysone + reduced 2,6-dichloroindophenol
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163% activity compared to O2
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-
r
ecdysteroids + O2
3-dehydroecdysteroids + H2O2
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structural requirements: alpha,beta-unsaturated keto function at C-6 and several hydroxyl functions including the hydroxyls at C-3 and at C-22
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?