Sequence of UBA1_HUMAN
EC Number:6.2.1.45
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
General information:
Sequence
0 MSSSPLSKKR RVSGPDPKPG SNCSPAQSVL SEVPSVPTNG MAKNGSEADI DEGLYSRQLY
60 VLGHEAMKRL QTSSVLVSGL RGLGVEIAKN IILGGVKAVT LHDQGTAQWA DLSSQFYLRE
120 EDIGKNRAEV SQPRLAELNS YVPVTAYTGP LVEDFLSGFQ VVVLTNTPLE DQLRVGEFCH
180 NRGIKLVVAD TRGLFGQLFC DFGEEMILTD SNGEQPLSAM VSMVTKDNPG VVTCLDEARH
240 GFESGDFVSF SEVQGMVELN GNQPMEIKVL GPYTFSICDT SNFSDYIRGG IVSQVKVPKK
300 ISFKSLVASL AEPDFVVTDF AKFSRPAQLH IGFQALHQFC AQHGRPPRPR NEEDAAELVA
360 LAQAVNARAL PAVQQNNLDE DLIRKLAYVA AGDLAPINAF IGGLAAQEVM KACSGKFMPI
420 MQWLYFDALE CLPEDKEVLT EDKCLQRQNR YDGQVAVFGS DLQEKLGKQK YFLVGAGAIG
480 CELLKNFAMI GLGCGEGGEI IVTDMDTIEK SNLNRQFLFR PWDVTKLKSD TAAAAVRQMN
540 PHIRVTSHQN RVGPDTERIY DDDFFQNLDG VANALDNVDA RMYMDRRCVY YRKPLLESGT
600 LGTKGNVQVV IPFLTESYSS SQDPPEKSIP ICTLKNFPNA IEHTLQWARD EFEGLFKQPA
660 ENVNQYLTDP KFVERTLRLA GTQPLEVLEA VQRSLVLQRP QTWADCVTWA CHHWHTQYSN
720 NIRQLLHNFP PDQLTSSGAP FWSGPKRCPH PLTFDVNNPL HLDYVMAAAN LFAQTYGLTG
780 SQDRAAVATF LQSVQVPEFT PKSGVKIHVS DQELQSANAS VDDSRLEELK ATLPSPDKLP
840 GFKMYPIDFE KDDDSNFHMD FIVAASNLRA ENYDIPSADR HKSKLIAGKI IPAIATTTAA
900 VVGLVCLELY KVVQGHRQLD SYKNGFLNLA LPFFGFSEPL AAPRHQYYNQ EWTLWDRFEV
960 QGLQPNGEEM TLKQFLDYFK TEHKLEITML SQGVSMLYSF FMPAAKLKER LDQPMTEIVS
1020 RVSKRKLGRH VRALVLELCC NDESGEDVEV PYVRYTIR
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Sequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
461815
Ayusawa D.,Kaneda S.,Itoh Y.,Yasuda H.,Murakami Y.,Sugasawa K.,Hanaoka F.,Seno T.
Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1.
Cell Struct. Funct.
17
113-122
1992
461816
Handley P.M.,Mueckler M.,Siegel N.R.,Ciechanover A.,Schwartz A.L.
Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.
Proc. Natl. Acad. Sci. U.S.A.
88
258-262
1991
461817
Handley P.M.,Mueckler M.,Siegel N.R.,Ciechanover A.,Schwartz A.L.
Proc. Natl. Acad. Sci. U.S.A.
88
7456-7456
1991
461818
Ross M.T.,Grafham D.V.,Coffey A.J.,Scherer S.,McLay K.,Muzny D.,Platzer M.,Howell G.R.,Burrows C.,Bird C.P.,Frankish A.,Lovell F.L.,Howe K.L.,Ashurst J.L.,Fulton R.S.,Sudbrak R.,Wen G.,Jones M.C.,Hurles M.E.,Andrews T.D.,Scott C.E.,Searle S.,Ramser J.,Whittaker A.,Deadman R.,Carter N.P.,Hunt S.E.,Chen R.,Cree A.,Gunaratne P.,Havlak P.,Hodgson A.,Metzker M.L.,Richards S.,Scott G.,Steffen D.,Sodergren E.,Wheeler D.A.,Worley K.C.,Ainscough R.,Ambrose K.D.,Ansari-Lari M.A.,Aradhya S.,Ashwell R.I.,Babbage A.K.,Bagguley C.L.,Ballabio A.,Banerjee R.,Barker G.E.,Barlow K.F.,Barrett I.P.,Bates K.N.,Beare D.M.,Beasley H.,Beasley O.,Beck A.,Bethel G.,Blechschmidt K.,Brady N.,Bray-Allen S.,Bridgeman A.M.,Brown A.J.,Brown M.J.,Bonnin D.,Bruford E.A.,Buhay C.,Burch P.,Burford D.,Burgess J.,Burrill W.,Burton J.,Bye J.M.,Carder C.,Carrel L.,Chako J.,Chapman J.C.,Chavez D.,Chen E.,Chen G.,Chen Y.,Chen Z.,Chinault C.,Ciccodicola A.,Clark S.Y.,Clarke G.,Clee C.M.,Clegg S.,Clerc-Blankenburg K.,Clifford K.,Cobley V.,Cole C.G.,Conquer J.S.,Corby N.,Connor R.E.,David R.,Davies J.,Davis C.,Davis J.,Delgado O.,Deshazo D.,Dhami P.,Ding Y.,Dinh H.,Dodsworth S.,Draper H.,Dugan-Rocha S.,Dunham A.,Dunn M.,Durbin K.J.,Dutta I.,Eades T.,Ellwood M.,Emery-Cohen A.,Errington H.,Evans K.L.,Faulkner L.,Francis F.,Frankland J.,Fraser A.E.,Galgoczy P.,Gilbert J.,Gill R.,Gloeckner G.,Gregory S.G.,Gribble S.,Griffiths C.,Grocock R.,Gu Y.,Gwilliam R.,Hamilton C.,Hart E.A.,Hawes A.,Heath P.D.,Heitmann K.,Hennig S.,Hernandez J.,Hinzmann B.,Ho S.,Hoffs M.,Howden P.J.,Huckle E.J.,Hume J.,Hunt P.J.,Hunt A.R.,Isherwood J.,Jacob L.,Johnson D.,Jones S.,de Jong P.J.,Joseph S.S.,Keenan S.,Kelly S.,Kershaw J.K.,Khan Z.,Kioschis P.,Klages S.,Knights A.J.,Kosiura A.,Kovar-Smith C.,Laird G.K.,Langford C.,Lawlor S.,Leversha M.,Lewis L.,Liu W.,Lloyd C.,Lloyd D.M.,Loulseged H.,Loveland J.E.,Lovell J.D.,Lozado R.,Lu J.,Lyne R.,Ma J.,Maheshwari M.,Matthews L.H.,McDowall J.,McLaren S.,McMurray A.,Meidl P.,Meitinger T.,Milne S.,Miner G.,Mistry S.L.,Morgan M.,Morris S.,Mueller I.,Mullikin J.C.,Nguyen N.,Nordsiek G.,Nyakatura G.,O'dell C.N.,Okwuonu G.,Palmer S.,Pandian R.,Parker D.,Parrish J.,Pasternak S.,Patel D.,Pearce A.V.,Pearson D.M.,Pelan S.E.,Perez L.,Porter K.M.,Ramsey Y.,Reichwald K.,Rhodes S.,Ridler K.A.,Schlessinger D.,Schueler M.G.,Sehra H.K.,Shaw-Smith C.,Shen H.,Sheridan E.M.,Shownkeen R.,Skuce C.D.,Smith M.L.,Sotheran E.C.,Steingruber H.E.,Steward C.A.,Storey R.,Swann R.M.,Swarbreck D.,Tabor P.E.,Taudien S.,Taylor T.,Teague B.,Thomas K.,Thorpe A.,Timms K.,Tracey A.,Trevanion S.,Tromans A.C.,d'Urso M.,Verduzco D.,Villasana D.,Waldron L.,Wall M.,Wang Q.,Warren J.,Warry G.L.,Wei X.,West A.,Whitehead S.L.,Whiteley M.N.,Wilkinson J.E.,Willey D.L.,Williams G.,Williams L.,Williamson A.,Williamson H.,Wilming L.,Woodmansey R.L.,Wray P.W.,Yen J.,Zhang J.,Zhou J.,Zoghbi H.,Zorilla S.,Buck D.,Reinhardt R.,Poustka A.,Rosenthal A.,Lehrach H.,Meindl A.,Minx P.J.,Hillier L.W.,Willard H.F.,Wilson R.K.,Waterston R.H.,Rice C.M.,Vaudin M.,Coulson A.,Nelson D.L.,Weinstock G.,Sulston J.E.,Durbin R.M.,Hubbard T.,Gibbs R.A.,Beck S.,Rogers J.,Bentley D.R.
The DNA sequence of the human X chromosome.
Nature
434
325-337
2005
461820
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Genome Res.
14
2121-2127
2004
461821
Zacksenhaus E.,Sheinin R.
Molecular cloning, primary structure and expression of the human X linked A1S9 gene cDNA which complements the ts A1S9 mouse L cell defect in DNA replication.
EMBO J.
9
2923-2929
1990
461823
Cook J.C.,Chock P.B.
Isoforms of mammalian ubiquitin-activating enzyme.
J. Biol. Chem.
267
24315-24321
1992
461824
Schwartz A.L.,Trausch J.S.,Ciechanover A.,Slot J.W.,Geuze H.
Immunoelectron microscopic localization of the ubiquitin-activating enzyme E1 in HepG2 cells.
Proc. Natl. Acad. Sci. U.S.A.
89
5542-5546
1992
461825
Handley-Gearhart P.M.,Stephen A.G.,Trausch-Azar J.S.,Ciechanover A.,Schwartz A.L.
Human ubiquitin-activating enzyme, E1. Indication of potential nuclear and cytoplasmic subpopulations using epitope-tagged cDNA constructs.
J. Biol. Chem.
269
33171-33178
1994
461826
Stephen A.G.,Trausch-Azar J.S.,Handley-Gearhart P.M.,Ciechanover A.,Schwartz A.L.
Identification of a region within the ubiquitin-activating enzyme required for nuclear targeting and phosphorylation.
J. Biol. Chem.
272
10895-10903
1997
461827
Giannakopoulos N.V.,Luo J.K.,Papov V.,Zou W.,Lenschow D.J.,Jacobs B.S.,Borden E.C.,Li J.,Virgin H.W.,Zhang D.E.
Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.
Biochem. Biophys. Res. Commun.
336
496-506
2005
461828
Rush J.,Moritz A.,Lee K.A.,Guo A.,Goss V.L.,Spek E.J.,Zhang H.,Zha X.-M.,Polakiewicz R.D.,Comb M.J.
Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.
Nat. Biotechnol.
23
94-101
2005
461829
Allen E.,Ding J.,Wang W.,Pramanik S.,Chou J.,Yau V.,Yang Y.
Gigaxonin-controlled degradation of MAP1B light chain is critical to neuronal survival.
Nature
438
224-228
2005
461830
Dephoure N.,Zhou C.,Villen J.,Beausoleil S.A.,Bakalarski C.E.,Elledge S.J.,Gygi S.P.
A quantitative atlas of mitotic phosphorylation.
Proc. Natl. Acad. Sci. U.S.A.
105
10762-10767
2008
461831
Gauci S.,Helbig A.O.,Slijper M.,Krijgsveld J.,Heck A.J.,Mohammed S.
Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
Anal. Chem.
81
4493-4501
2009
461832
Mayya V.,Lundgren D.H.,Hwang S.-I.,Rezaul K.,Wu L.,Eng J.K.,Rodionov V.,Han D.K.
Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
Sci. Signal.
2
RA46-RA46
2009
461833
Choudhary C.,Kumar C.,Gnad F.,Nielsen M.L.,Rehman M.,Walther T.C.,Olsen J.V.,Mann M.
Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Science
325
834-840
2009
461834
Olsen J.V.,Vermeulen M.,Santamaria A.,Kumar C.,Miller M.L.,Jensen L.J.,Gnad F.,Cox J.,Jensen T.S.,Nigg E.A.,Brunak S.,Mann M.
Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
Sci. Signal.
3
RA3-RA3
2010
461835
Burkard T.R.,Planyavsky M.,Kaupe I.,Breitwieser F.P.,Buerckstuemmer T.,Bennett K.L.,Superti-Furga G.,Colinge J.
Initial characterization of the human central proteome.
BMC Syst. Biol.
5
17-17
2011
461836
Rigbolt K.T.,Prokhorova T.A.,Akimov V.,Henningsen J.,Johansen P.T.,Kratchmarova I.,Kassem M.,Mann M.,Olsen J.V.,Blagoev B.
System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.
Sci. Signal.
4
RS3-RS3
2011
461837
Moudry P.,Lukas C.,Macurek L.,Hanzlikova H.,Hodny Z.,Lukas J.,Bartek J.
Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage.
Cell Cycle
11
1573-1582
2012
461838
Van Damme P.,Lasa M.,Polevoda B.,Gazquez C.,Elosegui-Artola A.,Kim D.S.,De Juan-Pardo E.,Demeyer K.,Hole K.,Larrea E.,Timmerman E.,Prieto J.,Arnesen T.,Sherman F.,Gevaert K.,Aldabe R.
N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.
Proc. Natl. Acad. Sci. U.S.A.
109
12449-12454
2012
461839
Zhou H.,Di Palma S.,Preisinger C.,Peng M.,Polat A.N.,Heck A.J.,Mohammed S.
Toward a comprehensive characterization of a human cancer cell phosphoproteome.
J. Proteome Res.
12
260-271
2013
461840
Bian Y.,Song C.,Cheng K.,Dong M.,Wang F.,Huang J.,Sun D.,Wang L.,Ye M.,Zou H.
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
J. Proteomics
96
253-262
2014
461841
Vaca Jacome A.S.,Rabilloud T.,Schaeffer-Reiss C.,Rompais M.,Ayoub D.,Lane L.,Bairoch A.,Van Dorsselaer A.,Carapito C.
N-terminome analysis of the human mitochondrial proteome.
Proteomics
15
2519-2524
2015
461842
Beck D.B.,Ferrada M.A.,Sikora K.A.,Ombrello A.K.,Collins J.C.,Pei W.,Balanda N.,Ross D.L.,Ospina Cardona D.,Wu Z.,Patel B.,Manthiram K.,Groarke E.M.,Gutierrez-Rodrigues F.,Hoffmann P.,Rosenzweig S.,Nakabo S.,Dillon L.W.,Hourigan C.S.,Tsai W.L.,Gupta S.,Carmona-Rivera C.,Asmar A.J.,Xu L.,Oda H.,Goodspeed W.,Barron K.S.,Nehrebecky M.,Jones A.,Laird R.S.,Deuitch N.,Rowczenio D.,Rominger E.,Wells K.V.,Lee C.R.,Wang W.,Trick M.,Mullikin J.,Wigerblad G.,Brooks S.,Dell'Orso S.,Deng Z.,Chae J.J.,Dulau-Florea A.,Malicdan M.C.V.,Novacic D.,Colbert R.A.,Kaplan M.J.,Gadina M.,Savic S.,Lachmann H.J.,Abu-Asab M.,Solomon B.D.,Retterer K.,Gahl W.A.,Burgess S.M.,Aksentijevich I.,Young N.S.,Calvo K.R.,Werner A.,Kastner D.L.,Grayson P.C.
Somatic Mutations in UBA1 and Severe Adult-Onset Autoinflammatory Disease.
N. Engl. J. Med.
383
2628-2638
2020
461843
Xie S.T.
Expression, purification, and crystal structure of N-terminal domains of human ubiquitin-activating enzyme (E1).
Biosci. Biotechnol. Biochem.
78
1542-1549
2014
461844
Ramser J.,Ahearn M.E.,Lenski C.,Yariz K.O.,Hellebrand H.,von Rhein M.,Clark R.D.,Schmutzler R.K.,Lichtner P.,Hoffman E.P.,Meindl A.,Baumbach-Reardon L.
Rare missense and synonymous variants in UBE1 are associated with X-linked infantile spinal muscular atrophy.
Am. J. Hum. Genet.
82
188-193
2008
461845
Dlamini N.,Josifova D.J.,Paine S.M.,Wraige E.,Pitt M.,Murphy A.J.,King A.,Buk S.,Smith F.,Abbs S.,Sewry C.,Jacques T.S.,Jungbluth H.
Clinical and neuropathological features of X-linked spinal muscular atrophy (SMAX2) associated with a novel mutation in the UBA1 gene.
Neuromuscul. Disord.
23
391-398
2013
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