EC Number   |
Protein Variants |
Reference |
|---|
   7.4.2.1 | A297V |
site-directed mutagenesis, the catalytically inactive mutant does not respond to complex amino acid mixtures and constitutively sorts Gap1p to the plasma membrane. The mutant shows reduced activity with altered specificity compared to the wild-type enzyme and does not transport basic amino acids, its trafficking is also not regulated by these amino acids. The mutant has a specific defect in transport of positively charged amino acids |
720414 |
| 7.4.2.1 | D149A |
mutation in histidine binding subunit HisJ, mutant protein is capable of interacting with HisQMP2 although at a much reduced level |
246887 |
| 7.4.2.1 | D149N |
mutation in histidine binding subunit HisJ, mutant protein is capable of interacting with HisQMP2 although at a much reduced level |
246887 |
| 7.4.2.1 | D61N |
mutation in the ATP-binding subunit HisP*, defective in ATP hydrolysis |
246886 |
| 7.4.2.1 | E191K |
mutation in the ATP-binding subunit HisP*, greater ease of HisP release from HisQM than in wild-type enzyme. Increased intrinsic ATPase activity without HisJ |
246886 |
| 7.4.2.1 | E202K |
mutation in the ATP-binding subunit HisP*, greater ease of HisP release from HisQM than in wild-type enzyme. Increased intrinsic ATPase activity without HisJ |
246886 |
| 7.4.2.1 | H211R |
mutation in the ATP-binding subunit HisP*, defective in ATP hydrolysis |
246886 |
| 7.4.2.1 | I394 |
site-directed mutagenesis, the mutation strongly affects both transport and signaling with citrulline and other amino acids |
700356 |
| 7.4.2.1 | K45P |
mutation in the ATP-binding subunit HisP*, defective in ATP hydrolysis |
246886 |
| 7.4.2.1 | K9R/K16R |
site-directed mutagenesis, the mutant shows reduced activity with altered specificity compared to the wild-type enzyme |
720414 |
