EC Number |
Protein Variants |
Reference |
---|
6.5.1.4 | C308A |
recombinant mutant protein |
651965 |
6.5.1.4 | D287A |
activity very low, 1% of wild-type enzyme |
710580 |
6.5.1.4 | D297A |
enzyme inactive |
710550 |
6.5.1.4 | E14A |
activity very low, only 4% of RNA is cyclized compared to 95% with wild-type enzyme |
710550 |
6.5.1.4 | E270A |
reduced activity compared to wild-type enzyme, about 80% |
710580 |
6.5.1.4 | F135A |
activity very low, 3% of wild-type enzyme |
710580 |
6.5.1.4 | F251A |
considerably reduced activity compared to wild-type enzyme, about 30% |
710580 |
6.5.1.4 | H309A |
activity very low, less than 10% of wild-type enzyme |
710580 |
6.5.1.4 | H309A |
mutant is unreactive with either 3'-phosphate RNA end or 2'-phosphate RNA end |
728788 |
6.5.1.4 | H309G |
enzyme inactive |
710580 |