EC Number   |
Protein Variants |
Reference |
|---|
    6.3.5.7 | D178E |
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable |
662450 |
| 6.3.5.7 | D178N |
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable |
662450 |
| 6.3.5.7 | E125D |
mutation in subunit B, molecular dynamics simulations |
-, 745612 |
| 6.3.5.7 | E125Q |
mutation in subunit B, molecular dynamics simulations |
-, 745612 |
| 6.3.5.7 | K236E/E328A |
mutant used for crystallization, secondary structure contents and enzymatic activities similar to wild-type |
743955 |
| 6.3.5.7 | K254E |
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable |
662450 |
| 6.3.5.7 | K88R |
mutation in subunit B, molecular dynamics simulations |
-, 745612 |
| 6.3.5.7 | more |
activity of the engineered enzyme variants indicates that the acceptor stem loop is the principle discrimination element because insertion of this loop alone enhances the specificity of the archaeal enzyme toward tRNAGln2 |
728668 |
| 6.3.5.7 | more |
construction of an an N-terminal deletion mutant lacking amino acids 1-186 corresponding to the eukaryote-specific protein domains. The domains substantially influence amino acid binding, tRNA binding and aminoacylation efficiency, but they play no role in either specific nucleotide readout or discrimination against noncognate tRNA |
-, 745552 |
| 6.3.5.7 | S128T |
mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln |
674787 |
