EC Number   |
Protein Variants |
Reference |
|---|
   6.3.5.11 | D45N |
below 0.02% of the amidation activity of wild-type, no change in the order of amidation |
661083 |
| 6.3.5.11 | D48N |
0.2% of the amidation activity of wild-type, no change in the order of amidation |
661083 |
| 6.3.5.11 | D97N |
32.4% of the amidation activity of wild-type, no change in the order of amidation |
661083 |
| 6.3.5.11 | E90Q |
below 0.02% of the amidation activity of wild-type, no change in the order of amidation |
661083 |
| 6.3.5.11 | L47A |
10.6% of the amidation activity of wild-type, specific decrease in the affinity of the enzyme for the c-monoamide intermediate |
661083 |
| 6.3.5.11 | more |
co-expression of the cobA gene from Propionibacterium freudenreichii and the cbiA, -C, -D, -E, -T, -F, -G, -H, -J, -K, -L, and -P genes from Salmonella enterica serovar typhimurium in Escherichia coli result in the production of cobyrinic acid a,c-diamide. Strains that have neither cbiP nor cbiA synthesized 1-desmethylcobyrinic acid even in the presence of cbiD, suggesting that CbiA and CbiP are necessary for CbiD activity |
708984 |
| 6.3.5.11 | more |
when the Bacillus megaterium cob operon is cloned into a plasmid and transformed into an Escherichia coli strain containing the Salmonella typhimurium cbiP, it confers upon the host strain the ability to make the cobyric acid de no.o. However, cobyric acid synthesis is observed only when the strain is grown anaerobically |
707417 |
| 6.3.5.11 | Y46A |
3.8% of the amidation activity of wild-type, specific decrease in the affinity of the enzyme for the c-monoamide intermediate |
661083 |
