EC Number   |
Protein Variants |
Reference |
|---|
    6.3.2.10 | A288T |
naturally occuring murF2 allele, expressed as a fusion protein with glutathione S-transferase, is 181fold less catalytically active than the wild-type enzyme at 30°c and even more reduced at 42°C |
649829 |
| 6.3.2.10 | E158A |
site-directed mutagenesis, 4520fold reduced activity compared to the wild-type |
649829 |
| 6.3.2.10 | E158D |
site-directed mutagenesis, 246fold reduced activity compared to the wild-type |
649829 |
| 6.3.2.10 | E158G |
site-directed mutagenesis, over 451fold reduced activity compared to the wild-type |
649829 |
| 6.3.2.10 | H188A |
site-directed mutagenesis, 203fold reduced activity compared to the wild-type |
649829 |
| 6.3.2.10 | H188D |
site-directed mutagenesis, 2990fold reduced activity compared to the wild-type |
649829 |
| 6.3.2.10 | H188G |
site-directed mutagenesis, 214fold reduced activity compared to the wild-type |
649829 |
| 6.3.2.10 | H188N |
site-directed mutagenesis, 1860fold reduced activity compared to the wild-type |
649829 |
| 6.3.2.10 | K202A |
site-directed mutagenesis, exchange of highly conserved lysine residue leads to highly reduced activity, activity can be rescued best by addition of propionate or other short-chain carboxylic acids, but only in a small extent by amines |
651110 |
