EC Number   |
Protein Variants |
Reference |
|---|
    6.2.1.26 | G154P |
site-directed mutagenesis, the mutant shows the same stability and circular dichroism spectra as the wild-type enzyme, but reduced catalytic activity |
745330 |
| 6.2.1.26 | G157P |
site-directed mutagenesis, the mutant shows the same stability and circular dichroism spectra as the wild-type enzyme, but reduced catalytic activity |
745330 |
| 6.2.1.26 | H341A |
mutant enzyme loses 65% of ist activity and the Km-value for ATP increases 5.4fold |
648944 |
| 6.2.1.26 | more |
identification of three aae14 mutant alleles by reverse genetics, that are seedling lethal, and show no detectable phylloquinon, phenotype, overview. Weak expression of an AAE14 transgene in mutant Arabidopsis thaliana plants, controlled by the uninduced XVE promoter, results in chlorotic, slow-growing plants that accumulate phylloquinone. Inducing the XVE promoter in these plants, or expressing an AAE14 transgene under the control of the CaMV 35S promoter, led to full complementation of the mutant phenotype, aae14-mutant plants are also able to synthesize phylloquinone when provided with 1,4-dihydroxy-2-naphthoate, an intermediate in phylloquinone synthesis downstream of the OSB-CoA ligase reaction, overview |
694676 |
| 6.2.1.26 | more |
mutation of the P-loop residues hydrogen-bonded to ATP reveals a crucial catalytic role of ATP-enzyme interaction |
-, 745330 |
| 6.2.1.26 | R382A |
site-directed mutagenesis, the mutant shows the same stability and circular dichroism spectra as the wild-type enzyme, but reduced catalytic activity |
-, 745330 |
| 6.2.1.26 | R382K |
site-directed mutagenesis, the mutant shows the same stability and circular dichroism spectra as the wild-type enzyme, but reduced catalytic activity |
745330 |
| 6.2.1.26 | T152A |
site-directed mutagenesis, the mutant shows the same stability and circular dichroism spectra as the wild-type enzyme, but reduced catalytic activity |
-, 745330 |
| 6.2.1.26 | T155A |
site-directed mutagenesis, the mutant shows the same stability and circular dichroism spectra as the wild-type enzyme, but reduced catalytic activity |
-, 745330 |
| 6.2.1.26 | T155A/T156A |
site-directed mutagenesis, the mutant shows the same stability and circular dichroism spectra as the wild-type enzyme, but reduced catalytic activity |
745330 |
