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Results 1 - 10 of 13 > >>
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1F261Y the mutant shows a complete loss of the ability to make D-alanyl-(R)-lactate 728168
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1more construction of several mutant strains, generating a Bacillus subtilis strain BP341A expressing a depsipeptide-containing (D-Ala-D-Lac) lipid II, phenotype, overview. Strains BP341A-E, that require expression of the VanB-type operon for growth, have mutations in the endogenous Ddl ligase -, 716269
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1more structure-based modification of D-alanine-D-alanine ligase from strain ATCC 43589 for depsipeptide synthesis, overview 714541
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1Q260K/A283E the two amino acid substitutions result from point mutations at nucleotide positions 778 and 848, respectively. The mutant enzyme VRSA-9 synthesizes precursors ending in D-Ala-D-Lac (72%) and D-Ala-D-Ala (21%) in the absence of vancomycin. VRSA-9 Ddl shows a 200fold loss of activity and the importance of conformational changes in the dimer interface which can indirectly affect the topology of the active site -, 715371
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1S137A site-directed mutagenesis, the mutant D-alanine-D-alanine ligase also shows formation of D-alanyl-D-lactate depsipeptide in contrast to the wild-type enzyme, EC 6.3.2.4, which is inactive with (R)-lactate 714541
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1S137A/Y207F site-directed mutagenesis, the mutant D-alanine-D-alanine ligase also shows formation of D-alanyl-D-lactate depsipeptide in contrast to the wild-type enzyme, EC 6.3.2.4, which is inactive with (R)-lactate 714541
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1S137F/Y207F site-directed mutagenesis, the mutant D-alanine-D-alanine ligase also shows formation of D-alanyl-D-lactate depsipeptide in contrast to the wild-type enzyme, EC 6.3.2.4, which is inactive with (R)-lactate 714541
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1S137G/Y207F site-directed mutagenesis, the mutant D-alanine-D-alanine ligase also shows formation of D-alanyl-D-lactate depsipeptide in contrast to the wild-type enzyme, EC 6.3.2.4, which is inactive with (R)-lactate 714541
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1S137T/Y207F site-directed mutagenesis, the mutant D-alanine-D-alanine ligase also shows formation of D-alanyl-D-lactate depsipeptide in contrast to the wild-type enzyme, EC 6.3.2.4, which is inactive with (R)-lactate 714541
Display the word mapDisplay the reaction diagram Show all sequences 6.1.2.1S150A mutant of D-Ala-D-Ala ligase Ddl, mutant has gained depsipeptide ligase activity, i.e. formation of D-Ala-D-Lac, D-Ala-D-hydroxybutyrate, with dipeptide/depsipeptide partition ratios that mimic the pH behaviour of D-Ala-D-lactate ligase VanA. Mutant displays a clear pH-dependent partitioning between the preferred depsipeptide product at low pH and the dipeptide product at high pH 707464
Results 1 - 10 of 13 > >>