EC Number |
Protein Variants |
Reference |
---|
6.1.1.27 | D418N/D420N/T423V |
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme, it is active with tRNA substrate containing unusual residues 7-(2-thienyl)-imidazo[4,5-b]pyridine and pyrrole-2-carbaldehyde in the anticodon, overview |
690738 |
6.1.1.27 | E418D |
site-directed mutagenesis, the mutant shows reduced activity and altered tRNA substrate specificity, compared to the wild-type enzyme |
676139 |
6.1.1.27 | E418D/E420D |
site-directed mutagenesis, the mutant shows reduced activity and altered tRNA substrate specificity, compared to the wild-type enzyme |
676139 |
6.1.1.27 | E418D/E420Q |
site-directed mutagenesis, the mutant shows reduced activity and altered tRNA substrate specificity, compared to the wild-type enzyme |
676139 |
6.1.1.27 | E418N |
site-directed mutagenesis, the mutant shows reduced activity and altered tRNA substrate specificity, compared to the wild-type enzyme |
676139 |
6.1.1.27 | E418N/E420D |
site-directed mutagenesis, the mutant shows reduced activity and altered tRNA substrate specificity, compared to the wild-type enzyme |
676139 |
6.1.1.27 | E418N/E420N |
site-directed mutagenesis, the mutant shows reduced activity and altered tRNA substrate specificity, compared to the wild-type enzyme |
676139 |
6.1.1.27 | E418N/E420N/T423V |
efficiently charged phosphoserine to tRNA containing the (pyrrole-2-carbaldehyde)UA anticodon |
690738 |
6.1.1.27 | E418N/E420N/T423V |
site-directed mutagenesis, the mutant shows reduced activity and altered tRNA substrate specificity, compared to the wild-type enzyme |
676139 |
6.1.1.27 | E418N/E420Q |
site-directed mutagenesis, the mutant shows reduced activity and altered tRNA substrate specificity, compared to the wild-type enzyme |
676139 |