EC Number |
Protein Variants |
Reference |
---|
6.1.1.18 | A29X |
site-directed mutagenesis |
716940 |
6.1.1.18 | C229R |
site-directed mutagenesis, transplanting the conserved arginine residue from glutamyl-tRNA synthetase, EC 6.1.1.17, to glutaminyltRNA synthetase improves the KM of GlnRS for noncognate glutamate |
694903 |
6.1.1.18 | C229R/Q255I |
site-directed mutagenesis, comparison of mutant activity with glutamate and glutamine to charge tRNAGln to the wild-type activity, the mutant shows no activity with L-Gln, but weakly with L-Glu |
694903 |
6.1.1.18 | C229R/Q255I/S227A/F233Y |
site-directed mutagenesis, comparison of mutant activity with glutamate and glutamine to charge tRNAGln to the wild-type activity, the mutant shows no activity with L-Gln, but activity with L-Glu |
694903 |
6.1.1.18 | cGluGlnRS |
a chimeric protein, consisting of the catalytic domain of GluRS and the anticodon-binding domain of GlnRS, is constructed |
703714 |
6.1.1.18 | D235A |
saturation mutagenesis, only little complementation of glnS-deficient strain |
649850 |
6.1.1.18 | D486R/L488Q |
the double mutant causes a relaxed tRNA anticodon specificity |
726984 |
6.1.1.18 | D66E |
saturation mutagenesis, 18fold increased Km for glutamine, decreased turnover |
649850 |
6.1.1.18 | D66F |
saturation mutagenesis, highly increased Km for glutamine, 1200fold decrease in activity |
649850 |
6.1.1.18 | D66G |
saturation mutagenesis, only little complementation of glnS-deficient strain |
649850 |