EC Number   |
Protein Variants |
Reference |
|---|
    6.1.1.16 | C209S |
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.0031% of the wild-type ratio |
661203 |
| 6.1.1.16 | C28S |
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.012% of the wild-type ratio |
661203 |
| 6.1.1.16 | C28S/C209S |
site-directed mutagenesis, no ATP-diphosphate exchange activity, residual aminoacylation activity |
652909 |
| 6.1.1.16 | C28S/C209S |
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.0026% of the wild-type ratio |
661203 |
| 6.1.1.16 | C28S/C209S/H234N/E238Q |
aminoacylation of tRNACys is not detectable |
661203 |
| 6.1.1.16 | C28S/C209S/H234N/E238Q |
site-directed mutagensis, mutation of all zinc binding ligands, complete loss of bound zinc, weak ability to bind serine, thus loss of amino acid substrate discrimination ability |
652909 |
| 6.1.1.16 | C36S/C214S/C244S |
site-directed mutagenesis, activity is similar to the wild-type enzyme, but the affinity for cysteine binding is increased |
652909 |
| 6.1.1.16 | D239A/D240A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
-, 672180 |
| 6.1.1.16 | D417A/E420A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
-, 672180 |
| 6.1.1.16 | D435A/D436A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
-, 672180 |
