EC Number |
Protein Variants |
Reference |
---|
5.4.99.64 | D117A |
mutation in subunit HcmA, results in significantly increased activity toward (R)-3-hydroxybutyryl-CoA |
738677 |
5.4.99.64 | D117A |
mutation in the large subunit HcmA results in significantly increased activity toward (R)-3-hydroxybutyryl-CoA |
738677 |
5.4.99.64 | D117V |
mutation in subunit HcmA, results in significantly increased activity toward (R)-3-hydroxybutyryl-CoA and high activity toward pivalyl-CoA |
738677 |
5.4.99.64 | D117V |
mutation in the large subunit HcmA results in significantly increased activity toward (R)-3-hydroxybutyryl-CoA. The mutant enzyme shows high activity toward pivalyl-CoA |
738677 |
5.4.99.64 | I90A |
mutation in the large subunit HcmA results in a significant reduction in conversion rates and diminution of the catalytic efficiencies for the main substrates of the wild-type enzyme, (S)-3-hydroxybutyryl-coA and 2-hydroxyisobutyryl-CoA. Wild-type enzyme as well as mutant enzyme can also isomerize pivalyl- and isovaleryl-CoA, albeit at more than 10times lower rates than the favorite substrate (S)-3-hydroxybutyryl-CoA |
738677 |
5.4.99.64 | I90A |
strong decrease in activity |
738677 |
5.4.99.64 | I90F |
complete loss of enzyme activity |
-, 736409 |
5.4.99.64 | I90L |
mutation in the large subunit HcmA results in a significant reduction in conversion rates and diminution of the catalytic efficiencies for the main substrates of the wild-type enzyme, (S)-3-hydroxybutyryl-coA and 2-hydroxyisobutyryl-CoA. Mutant enzyme does not show any significant activities with pivalyl-CoA and isovaleryl-CoA |
738677 |
5.4.99.64 | I90L |
strong decrease in activity |
738677 |
5.4.99.64 | I90V |
mutation in the large subunit HcmA, wild-type enzyme as well as mutant enzyme can also isomerize pivalyl- and isovaleryl-CoA, albeit at more than 10times lower rates than the favorite substrate (S)-3-hydroxybutyryl-CoA |
738677 |