EC Number   |
Protein Variants |
Reference |
|---|
   5.4.99.47 | F124L/F365L/I732A |
site-directed mutagenesis, the mutant shows reduced parkeol synthesis activity compared to the wild-type and synthesis of other triterpene products |
-, 748774 |
| 5.4.99.47 | H477N |
the mutant produces 88% lanosterol and 12% parkeol from oxidosqualene |
653405 |
| 5.4.99.47 | H477Q |
the mutant produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol from oxidosqualene |
653405 |
| 5.4.99.47 | Q450H |
nonviable single mutant produces minor amount achilleol A as the sole product |
728425 |
| 5.4.99.47 | Q450H/V454I |
mutant produces lanosterol, (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol, protosta-16,24-dien-3beta-ol in a ratio of 75:16:9 |
728425 |
| 5.4.99.47 | T384Y |
mutant produces lanosterol, parkeol, 9beta-lanosta-7,24-dien-3 beta-ol, protosta-13(17),24-dien-3beta-ol and protosta-16,24-dien-3beta-ol in the ratio of 63:31.5:3.5:1:1 without compounds achilleol A and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol |
728425 |
| 5.4.99.47 | T384Y/Q450H |
mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 1:98:1 |
728425 |
| 5.4.99.47 | T384Y/Q450H/V454I |
mutant generates parkeol as the sole product, Vmax/Km 18fold decreased for 3S-2,3-oxidosqualene compared to wild-type |
728425 |
| 5.4.99.47 | T384Y/V454I |
mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 10:84:6 |
728425 |
| 5.4.99.47 | V454I |
mutant produces poor amounts of lanosterol and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol (ratio 90:10) |
728425 |
