  5.4.99.45 | more |
the thermosensitive growth phenotype of deg1 mutants is specifically suppressible by overexpression of tRNAGln UUG but not tRNAGln CUG or any other tRNA carrying U38 or U39, which is modified by Deg1 to Psi, with clear suppression of deg1-induced thermosensitivity by multicopy tRNAGln UUG but not tRNAGln CUG or other tester tRNAs. Rescue by multicopy tRNAGln UUG is clearly incomplete as growth at 39°C is not fully restored to wild-type levels. Negative genetic interactions between DEG1, Elongator and URM1 can be extended to conditions of TORC1 inhibition, rapamycin sensitivity of deg1, urm1 and elp3 single and all possible double mutant combinations, overview. Deletion of the DEG1 gene in strain W303-1B and score of adenine auxotrophy as well as color colony in direct comparison with an elp3 mutant that is known to affect SUP4-mediated read-through |
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