EC Number   |
Protein Variants |
Reference |
|---|
   5.4.99.30 | D110A |
site-directed mutagenesis |
747867 |
| 5.4.99.30 | D111A |
site-directed mutagenesis |
747867 |
| 5.4.99.30 | D112A |
site-directed mutagenesis |
747867 |
| 5.4.99.30 | D112N |
activity is 131% of wild-type activity |
703076 |
| 5.4.99.30 | H273A |
site-directed mutagenesis |
747867 |
| 5.4.99.30 | more |
generation of RNAi transformants (NtUAM-KD) to downregulate all four of the UAM members, phenotype, genetic RNAi constructs, overview. Downregulation of NtUAMs expression effects on the localization of arabinan in leaf, and NtUAM knockdown causes the abnormal callus and hole in epidermal tissues |
748489 |
| 5.4.99.30 | more |
RNA interference (RNAi)-knockdown transformants (OsUAM3-KD) specific for OsUAM3. Modifications of cell wall polysaccharides at the cellular level are determined using antibodies against polysaccharides including Araf, low levels of arabinan in OsUAM3-KD pollen grains |
748926 |
| 5.4.99.30 | more |
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature |
-, 747867 |
| 5.4.99.30 | more |
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature. Targeted manipulation of UDP-sugar biosynthesis can result in biomass with substantially altered compositions |
747867 |
| 5.4.99.30 | R149A |
activity is 154.1% of wild-type activity |
703076 |
