EC Number   |
Protein Variants |
Reference |
|---|
    5.4.99.15 | A406 |
compared with wild-type enzyme, the hydrolysis:transglycosylation selectivity ratio is significantly increased |
-, 724886 |
| 5.4.99.15 | A406S |
the hydrolysis/transglycosylation selectivity ratio shows little change |
-, 724886 |
| 5.4.99.15 | D228N |
complete loss of both hydrolytic and transglycosylation activity |
680402 |
| 5.4.99.15 | D231A |
significant decrease of both hydrolytic and transglycosylation activity |
680402 |
| 5.4.99.15 | D275A |
about 50% and 40% decrease of hydrolytic and transglycosylation activity, resp. |
680402 |
| 5.4.99.15 | D394A |
significant decrease of both hydrolytic and transglycosylation activity |
680402 |
| 5.4.99.15 | D411A |
significant reductions in catalytic efficiency and increase in the transition-state energy |
-, 724886 |
| 5.4.99.15 | D411A |
significant reductions in catalytic efficiency, increase in the transition-state energy |
724886 |
| 5.4.99.15 | D443N |
complete loss of both hydrolytic and transglycosylation activity |
680402 |
| 5.4.99.15 | D610A |
significant reductions in catalytic efficiency and increase in the transition-state energy |
-, 724886 |
