EC Number   |
Protein Variants |
Reference |
|---|
   5.4.99.12 | C154A |
activity similar to wild-type |
704385 |
| 5.4.99.12 | C154A |
mutant shows high levels of activity |
704385 |
| 5.4.99.12 | C154S |
complete loss of activity, mutant is capable of binding to substrate tRNAPhe |
704385 |
| 5.4.99.12 | C154S |
no activity |
704385 |
| 5.4.99.12 | C169A |
activity similar to wild-type |
704385 |
| 5.4.99.12 | C169A |
mutant shows high levels of activity |
704385 |
| 5.4.99.12 | C169S |
complete loss of activity, mutant is unable to bind substrate tRNAPhe |
704385 |
| 5.4.99.12 | C169S |
no activity |
704385 |
| 5.4.99.12 | C55A/C154A/C169A |
activity similar to wild-type |
704385 |
| 5.4.99.12 | C55A/C154A/C169A |
only a small change in both Km and Vmax parameters as compared with wild-type enzyme. These mutations cause a 1.2fold increase in Km and a 1.6fold decrease in Vmax. The overall Vmax/Km ratio is lowered by a factor of 2 for the triple mutant |
704385 |
