EC Number   |
Protein Variants |
Reference |
|---|
   5.3.99.6 | C71A |
97.7% of wild-type activity, 94.1% yield of cis-(+)-12-oxophytodienoic acid |
692293 |
| 5.3.99.6 | C71S |
97.1% of wild-type activity, 93.9% yield of cis-(+)-12-oxophytodienoic acid |
692293 |
| 5.3.99.6 | C71Y |
33.3% of wild-type activity, 67.4% yield of cis-(+)-12-oxophytodienoic acid |
692293 |
| 5.3.99.6 | E23A |
29% of wild-type activity, 57.6% yield of cis-(+)-12-oxophytodienoic acid |
692293 |
| 5.3.99.6 | E23A |
complete loss of activity |
692293 |
| 5.3.99.6 | F140V |
site-directed mutagenesis, the mutant variant is active with the 13-hydroperoxy octadecatrienoic acid-derived allene oxide, but inactive with 20:4(n-6)-derived (12S)-hydroperoxy eicosatetraenoic acid and (12S)-hydroperoxy eicosatetraenoic acid-derived allene oxide |
728516 |
| 5.3.99.6 | F29I/F140V |
site-directed mutagenesis, the mutant variant is active with the 13-hydroperoxy octadecatrienoic acid-derived allene oxide, but inactive with 20:4(n-6)-derived (12S)-hydroperoxy eicosatetraenoic acid and (12S)-hydroperoxy eicosatetraenoic acid-derived allene oxide |
728516 |
| 5.3.99.6 | F43Y |
90.4% of wild-type activity, 93.5% yield of cis-(+)-12-oxophytodienoic acid |
692293 |
| 5.3.99.6 | F51A |
128.4% of wild-type activity, 94.7% yield of cis-(+)-12-oxophytodienoic acid |
692293 |
| 5.3.99.6 | F85A |
54.7% of wild-type activity, 71.7% yield of cis-(+)-12-oxophytodienoic acid |
692293 |
