EC Number |
Protein Variants |
Reference |
---|
5.1.1.8 | C236S |
radical loss of OH-L/D-Pro epimerization |
676749 |
5.1.1.8 | C88S |
radical loss of OH-L/D-Pro epimerization |
676749 |
5.1.1.8 | F240W |
mutant enzyme shows similar kinetic constants for proline and hydroxyproline as compared to the wild-type enzyme |
730678 |
5.1.1.8 | V60F |
radical loss of OH-L/D-Pro epimerization. V60 residue accounts for OH-Pro ligand specificity |
676749 |
5.1.1.8 | V60G |
almost complete loss of epimerization of trans-4-hydroxy-L-Pro, some residual activity on trans-4-hydroxy-D-Pro. V60 residue accounts for OH-Pro ligand specificity |
676749 |
5.1.1.8 | W241F the mutant enzyme |
shows 5.3fold, 430fold, and 5.8fold lower kcat/Km values for trans-4-hydroxy-L-proline, trans-3-hydroxy-L-proline, and cis-4-hydroxy-D-proline respectively, mainly due to a marked decrease in kcat values, whereas no significant effects were found in the kinetic constants of proline, suggesting that this (hydrophobic and bulky) tryptophan residue plays an importance role in the recognition of hydroxyproline (more hydrophilic and bulky than proline) |
-, 730678 |