EC Number |
Protein Variants |
Reference |
---|
4.3.1.19 | A14T |
the mutant enzyme shows higher thermostability than wild-type enzyme without considerable loss in activity |
747392 |
4.3.1.19 | E347F |
mutation decreases the K0.5 values of Thr without significant change of the n(H) value compared to wild-type. Mutant is strongly feedback-resistant to Ile compared to the wild-type enzyme. IC50 (Ile) is increased compared to wild-type |
729066 |
4.3.1.19 | E442A |
mutation increases the K0.5 value of Thr and n(H) value, comparing to those of the wild-type enzyme. IC50 (Ile) is decreased compared to wild-type |
729066 |
4.3.1.19 | E520A |
omr1-7 allele, tolerates high concentrations of L-O-methylthreonine |
663101 |
4.3.1.19 | F352A |
mutation decreases the K0.5 values of Thr without significant change of the n(H) value compared to wild-type. Mutant is strongly feedback-resistant to Ile compared to the wild-type enzyme. IC50 (Ile) is increased compared to wild-type |
729066 |
4.3.1.19 | F352A/I460F |
double mutant is more resistant to Ile inhibition than any single site mutant. Double mutations retains more than 85% activity even at 10 mM Ile |
729066 |
4.3.1.19 | F352A/R362F |
mutant shows both higher activity and stronger resistance to Ile inhibition compared to wild-type mice. Overexpression of mutant in Escherichia coli JW3591 significantly increases the production of ketobutyrate and Ile in comparison to the reference strains overexpressing wild-type |
729066 |
4.3.1.19 | F383A |
the mutant enzyme shows complete resistance to feedback inhibition by isoleucine |
-, 749422 |
4.3.1.19 | F510L |
mutant enzyme with greater thermostability compared to wild-type enzyme |
747392 |
4.3.1.19 | G323D |
mutant enzyme with greater thermostability compared to wild-type enzyme |
747392 |