EC Number   |
Protein Variants |
Reference |
|---|
   4.2.3.9 | D115E |
kcat/KM is 12fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 75:6:119 |
665301 |
| 4.2.3.9 | D115N |
inactive mutant enzyme |
665301 |
| 4.2.3.9 | D116E |
kcat/KM is 60fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 62:3:35 |
665301 |
| 4.2.3.9 | D116N |
kcat/KM is 48fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 63:2:35 |
665301 |
| 4.2.3.9 | D203L |
approximately 150fold decrease in kcat/KM |
729580 |
| 4.2.3.9 | E119D |
kcat/KM is 2.4fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 94:2:4 |
665301 |
| 4.2.3.9 | E119Q |
kcat/KM is 2.3fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 84:2:14 |
665301 |
| 4.2.3.9 | E227D |
kcat/KM is 1182fold higher than wild-type value, in contrast to wild-type enzyme that exclusively produces aristolochene from trans,trans-farnesyl diphosphate, the mutant enzyme produces 26% aristolochene and 74% germacrene A |
665301 |
| 4.2.3.9 | E227Q |
inactive mutant enzyme |
665301 |
| 4.2.3.9 | E252D |
kcat/KM is 111fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 19:0:81 |
665301 |
