EC Number |
Protein Variants |
Reference |
---|
4.2.3.5 | D367A |
comparable to the wild-type enzyme with respect to substrate and cofactor binding, but activity significantly lower |
678265 |
4.2.3.5 | D367N |
comparable to the wild-type enzyme with respect to substrate and cofactor binding, but activity significantly lower |
678265 |
4.2.3.5 | H106A |
activity 10fold decreased |
679365 |
4.2.3.5 | H106A |
mutant enzyme with 20fold reduced activity |
652570 |
4.2.3.5 | H17A |
activity 20fold decreased |
679365 |
4.2.3.5 | H17A |
mutant enzyme with 10fold reduced activity |
652570 |
4.2.3.5 | S127A |
amino acid replacements are performed using the QuikChange site-directed mutagenesis kit |
692285 |
4.2.3.5 | S16A |
amino acid replacements are performed using the QuikChange site-directed mutagenesis kit |
692285 |
4.2.3.5 | S16A/S127A |
double-mutant protein |
692285 |