EC Number   |
Protein Variants |
Reference |
|---|
  4.2.3.122 | H579A |
site-directed mutagenesis, the mutant produces only 56.3% S-limonene |
748889 |
| 4.2.3.122 | L423A/S454A |
site-directed mutagenesis, mutation M2, the mutant shows altered substrate specificity and product profile compared to the wild-type enzyme |
748889 |
| 4.2.3.122 | M458A |
site-directed mutagenesis, the mutant produces only 3.2% S-limonene |
748889 |
| 4.2.3.122 | more |
S-limonene synthase is converted to pinene or phellandrene synthases by site-directed mutagenesis, product profiles of mutant enzymes, overview |
748889 |
| 4.2.3.122 | N345A |
site-directed mutagenesis, the mutant produces only 20.9%imonene |
748889 |
| 4.2.3.122 | N345A |
site-directed mutagenesis, the mutation enlarges the active site, the mutant shows altered substrate specificity and product profilecompared to the wild-type enzyme |
748889 |
| 4.2.3.122 | N345A/L423A/S454A |
site-directed mutagenesis, mutation M3 enlarges the active site, the mutant shows altered substrate specificity and product profile compared to the wild-type enzyme, the mutant acts as a pinene synthase and produces about 70% pinenes and has about 2fold increase in the yield of overall terpene products |
748889 |
| 4.2.3.122 | N345A/L423A/S454G |
site-directed mutagenesis, mutation M5, the mutant shows altered substrate specificity and product profile compared to the wild-type enzyme, products of M5 are composed of 9.8% alpha-pinene, 52.7% beta-pinene and 13.4% limonene, it has about 2fold increase in the yield of overall terpene products |
748889 |
| 4.2.3.122 | N345G/L423A/S454A |
site-directed mutagenesis, mutation M4, the mutant shows altered substrate specificity and product profile compared to the wild-type enzyme, products of M4 are composed of 18.81% alpha-pinene, 44.00% beta-pinene, and 16.2% limonene |
748889 |
| 4.2.3.122 | S454A |
site-directed mutagenesis, the mutant produces only 73.7% S-limonene |
748889 |
